scholarly journals Effects of sildenafil and tadalafil on skin flap viability

2021 ◽  
Author(s):  
Rafael A. C. Souza ◽  
Carla Patrícia Martinelli-Kläy ◽  
Armando J. d’Acampora ◽  
Geraldo J. S. Bernardes ◽  
Sandro M. Sgrott ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Surgical Procedure ◽  
Wistar Rats ◽  
Skin Flap ◽  
Phosphodiesterase Inhibitor ◽  
Vascular Complication ◽  
Action Mechanisms ◽  
Flap Viability ◽  
Histological Processing ◽  
Positive Results

AbstractVascular complication is one of the causes of skin flap healing failure. Sildenafil and tadalafil, a type-5 phosphodiesterase inhibitor, can improve flap viability, however, the action mechanisms involved in this process are still unclear. To assess the effects of orally administered sildenafil and tadalafil on the healing kinetics and skin flap viability, sixty-two Wistar rats were divided into three groups: control (n = 22), sildenafil (n = 20), and tadalafil (n = 20). The solutions were administered orally (dose: 10 mg/kg) immediately after the surgical procedure and then every 24 h. At postoperative days 7 and 14, the skin flap samples were collected, submitted to histological processing and evaluated under optical microscopy. In experimental groups (sildenafil and tadalafil), we found an increased vascularization (p < 0.05) on the 7th and 14th day associated with the ulcer size decrease on the 14th day, although it was not significant. There was a higher influx of neutrophils and a decrease of mononuclear population on the 7th day (p < 0.05). On the 14th day, these differences were observed only in the tadalafil group (p < 0.05). This study suggested positive results with the use of sildenafil and tadalafil as adjuvant drugs in skin flap viability.

scholarly journals Capsaicin on the viability of random-pattern skin flaps in rats

2010 ◽  
Vol 25 (5) ◽  
pp. 440-443 ◽  
Author(s):  
Gustavo Roberto de Godoy ◽  
Richard Eloin Liebano ◽  
Juliana Barbosa Corrêa ◽  
Bernardo Hochman ◽  
Lydia Masako Ferreira
Keyword(s):  
Surgical Procedure ◽  
Wistar Rats ◽  
Skin Flap ◽  
Donor Site ◽  
Control Group ◽  
Random Pattern ◽  
Skin Flaps ◽  
Flap Necrosis ◽  
Group A

PURPOSE: To evaluate the effects of capsaicin on the viability of ischemic random-pattern skin flaps in rats. METHODS:Forty EPM1-Wistar rats were randomized into two groups of 20 animals each, the capsaicin group and the control group. A random-pattern skin flap measuring 10 x 4cm was raised and a plastic barrier was placed between the flap and the donor site. After the surgical procedure, the control group was treated with an inert vehicle in the form of a cream applied uniformly to a rayon bandage which, in turn, was applied to the surface of the skin flap. The capsaicin group was treated in the same way, but in this case capsaicin was added to the cream. This procedure was repeated for two consecutive days. RESULTS: There was a significantly smaller amount of flap necrosis in the capsaicin group (35.07%) than in the control group (44.75%) (p=0.035). CONCLUSION:Topical administration of capsaicin improved the viability of ischemic random-pattern skin flaps in rats.

scholarly journals Dermatosurgery Rounds - The Island SKIN Infraorbital Flap

2017 ◽  
Vol 5 (4) ◽  
pp. 554-555
Author(s):  
Georgi Tchernev ◽  
Serena Gianfaldoni ◽  
Uwe Wollina ◽  
Torello Lotti ◽  
Jacopo Lotti ◽  
...  
Keyword(s):  
Basal Cell Carcinoma ◽  
Surgical Procedure ◽  
Tumour Size ◽  
Skin Flap ◽  
Cosmetic Outcome ◽  
Dermatologic Surgery ◽  
Complete Excision ◽  
Facial Defects ◽  
Secondary Healing ◽  
Reconstructive Methods

The main objective in dermatologic surgery is complete excision of the tumour while achieving the best possible functional and cosmetic outcome. Also we must take into account age, sex, and tumour size and site. We should also consider the patient's expectations, the preservation of the different cosmetic units, and the final cosmetic outcome. Various reconstructive methods ranging from secondary healing to free flap applications are usedfor the reconstruction of perinasal or facial defects caused by trauma or tumour surgery. Herein, we describe the nasal infraorbital island skin flap for the reconstruction in a patient with basal cell carcinoma. No complications were observed in operation field. The infraorbital island skin flap which we describe for the perinasal area reconstruction is a safe, easily performed and versatile flap. The multidimensional use of this flap together with a relatively easy reconstruction plan and surgical procedure would be effective in flap choice.

scholarly journals The effect of ovariectomy on the skin flap viability and myeloperoxidase levels

2014 ◽  
Vol 115 (12) ◽  
pp. 766-770
Author(s):  
A. Coskun ◽  
D. C. Arikan ◽  
Y. K. Coban ◽  
H. Sayar ◽  
M. Kilinc ◽  
...  
Keyword(s):  
Skin Flap ◽  
Flap Viability

scholarly journals Effects of sildenafil on the viability of random skin flaps

2011 ◽  
Vol 26 (4) ◽  
pp. 314-319 ◽  
Author(s):  
Sumara Marques Barral ◽  
Ivana Duval Araujo ◽  
Paula Vieira Teixeira Vidigal ◽  
Cláudio Alvarenga Campos Mayrink ◽  
Adriana Duval Araujo ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Histological Analysis ◽  
Saline Solution ◽  
Skin Flap ◽  
Study Group ◽  
Tissue Formation ◽  
Skin Flaps ◽  
Dorsal Skin ◽  
Macroscopic Analysis

PURPOSE: To assess the viability of McFarlane skin flaps in rats with administration of sildenafil. METHODS: Twenty Wistar rats were distributed into two groups: Control (dorsal skin flap, subdermal application of saline solution at 0.9%) and Study (dorsal skin flap, subdermal application of sildenafil). Seven days after the surgery, flaps were photographed and graphically rendered. Then, they were analyzed with AutoCAD software. Three biopsies (proximal, medial and distal) of each flap were collected for histological analysis. RESULTS: Macroscopic analysis showed that animals of the study group had greater necrotic areas (p=0.003) in the dorsal skin flaps. Additionally, histological analysis of the distal third of these flaps showed a tendency to less granulated tissue formation in animals treated with sildenafil. CONCLUSION: Sildenafil subdermally was associated with lower viability of the random skin flap in rats.

Reliable Assessment of Skin Flap Viability Using Orthogonal Polarization Imaging

2003 ◽  
Vol 112 (2) ◽  
pp. 547-555 ◽  
Author(s):  
Wendy-Ann M. Olivier ◽  
Alexes Hazen ◽  
Jamie P. Levine ◽  
Hooman Soltanian ◽  
Seum Chung ◽  
...  
Keyword(s):  
Skin Flap ◽  
Orthogonal Polarization ◽  
Polarization Imaging ◽  
Flap Viability ◽  
Reliable Assessment

Third Place — Resident Clinical Science Award 1993: Effect of Pentoxyfylline on Myocutaneous Flap Viability in Pigs

1993 ◽  
Vol 109 (4) ◽  
pp. 668-675 ◽  
Author(s):  
Michael Armstrong ◽  
Dario R. Kunar ◽  
Charles W. Cummings
Keyword(s):  
Laser Doppler Velocimetry ◽  
Myocutaneous Flap ◽  
Skin Flap ◽  
Third Place ◽  
Myocutaneous Flaps ◽  
Partial Necrosis ◽  
Flap Viability ◽  
Before And After ◽  
The Mean ◽  
Double Blinded

Partial necrosis of a skin flap can complicate reconstructive surgery. We performed a double-blinded crossover study to determine if pentoxifylline improves perfusion and survival of a myocutaneous flap. Ten 20-kg pigs were fed pentoxifylline (400 mg three times a day) or placebo for 1 week before and after raising a 5 × 30 cm panniculus carnosus flap on one flank. After 1 week of washout, each pig began the opposite drug treatment and the surgery was repeated on the opposite flank. Immediately after surgery, perfusion dermofluorometry and laser Doppler velocimetry demonstrated a significant increase in the perfusion of pentoxifylline-treated flaps compared to control flaps. On postoperative day 7, the mean area of clinical necrosis was 39.7 ± 4.7 cm2 on the placebo sides and 30.1 ± 4.6 cm2 on the pentoxifylline sides ( f = 2.21, p < 0.05). We conclude that pentoxifylline improves perfusion and survival of myocutaneous flaps in pigs. Clinical trials appear to be indicated on the basis of the findings of this experiment

scholarly journals Effects of ionizing radiation on bone neoformation: histometric study in Wistar rats tibiae

2011 ◽  
Vol 26 (6) ◽  
pp. 475-480 ◽  
Author(s):  
Susette Cavicchioli Lucatto ◽  
Arnaldo Guilherme ◽  
Luciano Lauria Dib ◽  
Helena Regina Comodo Segreto ◽  
Maria Tereza de Seixas Alves ◽  
...  
Keyword(s):  
Radiation Dose ◽  
Ionizing Radiation ◽  
Radiation Effects ◽  
Wistar Rats ◽  
Bone Cells ◽  
Secondary Radiation ◽  
Male Wistar Rats ◽  
Histological Processing ◽  
The Right ◽  
Single Dosage

PURPOSE: Comparing the ionizing radiation effects on bone neoformation of rats tibiae previously submitted to radiotherapy with a single dosage of 30Gy with the contralateral tibiae that have received secondary radiation. METHODS: In thirty male Wistar rats, 30 days before surgical procedure when round defects would be created on the bone, the right tibia was irradiated with 30Gy and the left tibia received a calculated secondary radiation dose of 7Gy. Sacrifices were performed after 4, 7, 14, 21, 56 and 84 postoperative days and both tibiae were removed for histological processing. RESULTS: The left tibiae that received the dose of 7Gy has shown more bone neoformation from 14th postoperative days, giving evidences of less damage to cellular population responsible by bone neoformation. On the other hand, the dose of 30Gyon right tibiae did not exhibit significant differences among the periods, suggesting damage of long-lasting or even permanent duration. CONCLUSION: Tibiae submitted to radiation dose of 30Gy have shown more damage to bone cells than tibiae that received secondary radiation dose of 7Gy, especially observed on 14th, 56th and 84th postoperative days.

scholarly journals FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival

2019 ◽  
Vol 10 (12) ◽  
Author(s):  
Kailiang Zhou ◽  
Huanwen Chen ◽  
Jinti Lin ◽  
Hui Xu ◽  
Hongqiang Wu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Nuclear Translocation ◽  
Skin Flap ◽  
Therapeutic Benefit ◽  
Random Pattern ◽  
Skin Flaps ◽  
Flap Survival ◽  
Ampk Signaling ◽  
Flap Viability ◽  
Mechanistic Investigation

AbstractRandom-pattern skin flap is commonly used for surgical tissue reconstruction due to its ease and lack of axial vascular limitation. However, ischemic necrosis is a common complication, especially in distal parts of skin flaps. Previous studies have shown that FGF21 can promote angiogenesis and protect against ischemic cardiovascular disease, but little is known about the effect of FGF21 on flap survival. In this study, using a rat model of random skin flaps, we found that the expression of FGF21 is significantly increased after establishment skin flaps, suggesting that FGF21 may exert a pivotal effect on flap survival. We conducted experiments to elucidate the role of FGF21 in this model. Our results showed that FGF21 directly increased the survival area of skin flaps, blood flow intensity, and mean blood vessel density through enhancing angiogenesis, inhibiting apoptosis, and reducing oxidative stress. Our studies also revealed that FGF21 administration leads to an upregulation of autophagy, and the beneficial effects of FGF21 were reversed by 3-methyladenine (3MA), which is a well-known inhibitor of autophagy, suggesting that autophagy plays a central role in FGF21’s therapeutic benefit on skin flap survival. In our mechanistic investigation, we found that FGF21-induced autophagy enhancement is mediated by the dephosphorylation and nuclear translocation of TFEB; this effect was due to activation of AMPK-FoxO3a-SPK2-CARM1 and AMPK-mTOR signaling pathways. Together, our data provides novel evidence that FGF21 is a potent modulator of autophagy capable of significantly increasing random skin flap viability, and thus may serve as a promising therapy for clinical use.

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