scholarly journals Effects of sildenafil on the viability of random skin flaps

2011 ◽  
Vol 26 (4) ◽  
pp. 314-319 ◽  
Author(s):  
Sumara Marques Barral ◽  
Ivana Duval Araujo ◽  
Paula Vieira Teixeira Vidigal ◽  
Cláudio Alvarenga Campos Mayrink ◽  
Adriana Duval Araujo ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Histological Analysis ◽  
Saline Solution ◽  
Skin Flap ◽  
Study Group ◽  
Tissue Formation ◽  
Skin Flaps ◽  
Dorsal Skin ◽  
Macroscopic Analysis

PURPOSE: To assess the viability of McFarlane skin flaps in rats with administration of sildenafil. METHODS: Twenty Wistar rats were distributed into two groups: Control (dorsal skin flap, subdermal application of saline solution at 0.9%) and Study (dorsal skin flap, subdermal application of sildenafil). Seven days after the surgery, flaps were photographed and graphically rendered. Then, they were analyzed with AutoCAD software. Three biopsies (proximal, medial and distal) of each flap were collected for histological analysis. RESULTS: Macroscopic analysis showed that animals of the study group had greater necrotic areas (p=0.003) in the dorsal skin flaps. Additionally, histological analysis of the distal third of these flaps showed a tendency to less granulated tissue formation in animals treated with sildenafil. CONCLUSION: Sildenafil subdermally was associated with lower viability of the random skin flap in rats.

scholarly journals Morphological alterations in the heart and aorta of rats treated with glucocorticoids

2015 ◽  
Vol 32 (04) ◽  
pp. 231-235
Author(s):  
R. Dantas ◽  
K. Souza ◽  
D. Santos ◽  
V. Feitosa ◽  
E. Fioretto ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Histological Analysis ◽  
Saline Solution ◽  
Morphological Changes ◽  
Morphological Structure ◽  
Control Group ◽  
Morphological Alterations ◽  
Heart Chamber ◽  
Male Wistar Rats ◽  
Synthetic Glucocorticoid

Abstract Introduction: The objective of this study was to analyze the morphological structure of the heart and aorta of rats treated with the synthetic glucocorticoid dexamethasone. Material and Methods: Male Wistar rats were divided into two groups: 08 control rats undergoing treatment with a 0.9% saline solution for 10 days and 08 rats treated for 10 days with dexamethasone (2mg/kg animal weight). Results: Histological analysis detected a mild cardiac hypertrophy and 15% reduction of collagen located in the aorta of animals treated with glucocorticoid when compared to the control group. Conclusion: We conclude that treatment with dexamethasone for a period of 10 consecutive days is able to promote morphological changes in the structure of the heart chamber and, impair morphological structure of aorta.

scholarly journals Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats

2017 ◽  
Vol 44 (5) ◽  
pp. 457-464 ◽  
Author(s):  
Stephanie Luzia da Costa Pedretti ◽  
Cícero de Lima Rena ◽  
Maria Christina Marques Nogueira Castãnon ◽  
Ana Paula do Nascimento Duque ◽  
Fernando Henrique Pereira ◽  
...  
Keyword(s):  
Single Dose ◽  
Tissue Repair ◽  
Wistar Rats ◽  
Inflammatory Infiltrate ◽  
Histological Analysis ◽  
Distal Segment ◽  
Repair Process ◽  
Control Group ◽  
Skin Flaps ◽  
Cutaneous Flap

ABSTRACT Objectives: to verify the influence of dimethylsulfoxide and pentoxifylline on the vitality of cutaneous flaps in rats and the tissue repair process. Methods: were studied 30 Wistar rats, submitting them to a 2cm wide by 8cm long dorsal cutaneous flap, of caudal base. We distributed the animals in three groups: Control Group (n=10) with application gauze moistened with 0.9% Saline in the flap bed for 30 seconds; Dimethylsulfoxide group (n=10), with administration of 1ml of 5% dimethylsulfoxide divided into five injections of 0.2ml in the transition of the flap segments; Pentoxifylline group (n=10), with administration of pentoxifylline 20mg/kg, diluted to 1ml and divided into five injections of 0.2ml in the transition of the flap segments. Drugs were administered intraoperatively, in a single dose and subcutaneously. We observed the skin flaps for changes in color and texture. On the 10th postoperative day, we checked the dimensions of viable and necrotic tissues, followed by excision of the specimen for histological analysis. Results: the measurements of length of the viable and necrotic tissues between groups showed no differences. Histological analysis showed that the Dimethylsulfoxide group presented neovascularization, inflammatory infiltrate with leukocytes and more structured conjunctival stroma. The Pentoxifylline group showed neovascularization and inflammatory infiltrate, with moderate to intense granulation. The control group evolved with a higher rate of necrosis in the distal segment. Conclusion: dimethylsulfoxide and pentoxifylline influenced the vitality of the flap and the tissue repair process. However, they did not prevent necrosis macroscopically.

scholarly journals Capsaicin on the viability of random-pattern skin flaps in rats

2010 ◽  
Vol 25 (5) ◽  
pp. 440-443 ◽  
Author(s):  
Gustavo Roberto de Godoy ◽  
Richard Eloin Liebano ◽  
Juliana Barbosa Corrêa ◽  
Bernardo Hochman ◽  
Lydia Masako Ferreira
Keyword(s):  
Surgical Procedure ◽  
Wistar Rats ◽  
Skin Flap ◽  
Donor Site ◽  
Control Group ◽  
Random Pattern ◽  
Skin Flaps ◽  
Flap Necrosis ◽  
Group A

PURPOSE: To evaluate the effects of capsaicin on the viability of ischemic random-pattern skin flaps in rats. METHODS:Forty EPM1-Wistar rats were randomized into two groups of 20 animals each, the capsaicin group and the control group. A random-pattern skin flap measuring 10 x 4cm was raised and a plastic barrier was placed between the flap and the donor site. After the surgical procedure, the control group was treated with an inert vehicle in the form of a cream applied uniformly to a rayon bandage which, in turn, was applied to the surface of the skin flap. The capsaicin group was treated in the same way, but in this case capsaicin was added to the cream. This procedure was repeated for two consecutive days. RESULTS: There was a significantly smaller amount of flap necrosis in the capsaicin group (35.07%) than in the control group (44.75%) (p=0.035). CONCLUSION:Topical administration of capsaicin improved the viability of ischemic random-pattern skin flaps in rats.

Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Olugbemi T. Olaniyan ◽  
Olakunle A. Ojewale ◽  
Ayobami Dare ◽  
Olufemi Adebayo ◽  
Joseph E. Enyojo ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Cocos Nucifera ◽  
Reproductive Toxicity ◽  
Positive Control ◽  
Negative Control ◽  
Male Wistar Rats ◽  
Cocos Nucifera L

Abstract Objectives Lead primarily affects male reproductive functions via hormonal imbalance and morphological damage to the testicular tissue with significant alteration in sperm profile and oxidative markers. Though, different studies have reported that Cocos nucifera L. oil has a wide range of biological effects, this study aimed at investigating the effect of Cocos nucifera L. oil on lead acetate-induced reproductive toxicity in male Wistar rats. Methods Twenty (20) sexually matured male Wistar rats (55–65 days) were randomly distributed into four groups (n=5). Group I (negative control)—distilled water orally for 56 days, Group II (positive control)—5 mg/kg bwt lead acetate intraperitoneally (i.p.) for 14 days, Group III—6.7 mL/kg bwt Cocos nucifera L. oil orally for 56 days and Group IV—lead acetate intraperitoneally (i.p.) for 14 days and Cocos nucifera L. oil for orally for 56 days. Rats were sacrificed by diethyl ether, after which the serum, testis and epididymis were collected and used for semen analysis, biochemical and histological analysis. Results The lead acetate significantly increases (p<0.05) testicular and epididymal malondialdehyde (MDA) levels, while a significant reduction (p<0.05) in sperm parameters, organ weight, testosterone and luteinizing hormone was observed when compared with the negative control. The coadministration of Cocos nucifera oil with lead acetate significantly increases (p<0.05) testosterone, luteinizing hormone, sperm parameters and organ weight, with a significant decrease (p<0.05) in MDA levels compared with positive control. Histological analysis showed that lead acetate distorts testicular cytoarchitecture and germ cell integrity while this was normalized in the cotreated group. Conclusions Cocos nucifera oil attenuates the deleterious effects of lead acetate in male Wistar rats, which could be attributed to its polyphenol content and antioxidant properties.

scholarly journals Preclinical efficacy of stem cell therapy for skin flap: a systematic review and meta-analysis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yuan Li ◽  
Qi-lin Jiang ◽  
Leanne Van der Merwe ◽  
Dong-hao Lou ◽  
Cai Lin
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Stem Cell ◽  
Survival Rate ◽  
Meta Analysis ◽  
Skin Flap ◽  
Cochrane Library ◽  
Skin Flaps ◽  
Cell Based Therapy ◽  
Stem Cell Treatment

Abstract Background A skin flap is one of the most critical surgical techniques for the restoration of cutaneous defects. However, the distal necrosis of the skin flap severely restricts the clinical application of flap surgery. As there is no consensus on the treatment methods to prevent distal necrosis of skin flaps, more effective and feasible interventions to prevent skin flaps from necrosis are urgently needed. Stem therapy as a potential method to improve the survival rate of skin flaps is receiving increasing attention. Methods This review followed the recommendations from the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) statements. Twenty studies with 500 animals were included by searching Web of Science, EMBASE, PubMed, and Cochrane Library databases, up until October 8, 2020. Moreover, the references of the included articles were searched manually to obtain other studies. All analyses were conducted using Review Manager V.5.3 software. Results Meta-analysis of all 20 studies demonstrated stem cell treatment has significant effects on reducing necrosis of skin flap compared with the control group (SMD: 3.20, 95% CI 2.47 to 3.93). Besides, subgroup analysis showed differences in the efficacy of stem cells in improving the survival rate of skin flaps in areas of skin flap, cell type, transplant types, and method of administration of stem cells. The meta-analysis also showed that stem cell treatment had a significant effect on increasing blood vessel density (SMD: 2.96, 95% CI 2.21 to 3.72) and increasing the expression of vascular endothelial growth factor (VEGF, SMD: 4.34, 95% CI 2.48 to 6.1). Conclusions The preclinical evidence of our systematic review indicate that stem cell-based therapy is effective for promoting early angiogenesis by up regulating VEGF and ultimately improving the survival rate of skin flap. In summary, small area skin flap, the administration method of intra-arterial injection, ASCs and MSCs, and xenogenic stem cells from humans showed more effective for the survival of animal skin flaps. In general, stem cell-based therapy may be a promising method to prevent skin flap necrosis.

Effect of Hyperthermic Preconditioning on the Survival of Ischemia-Reperfused Skin Flaps: A New Skin-Flap Model in the Mouse

2002 ◽  
Vol 18 (2) ◽  
pp. 115-120 ◽  
Author(s):  
Trinh Cao Minh ◽  
Shigeru Ichioka ◽  
Takashi Nakatsuka ◽  
Junsuke Kawai ◽  
Masahiro Shibata ◽  
...  
Keyword(s):  
Skin Flap ◽  
Skin Flaps ◽  
Hyperthermic Preconditioning ◽  
New Skin

Polidocanol injection for chemical delay and its effect on the survival of rat dorsal skin flaps

2014 ◽  
Vol 67 (6) ◽  
pp. 851-856 ◽  
Author(s):  
Gülsüm Tetik Menevşe ◽  
Ali TeomanTellioglu ◽  
Nurgül Altuntas ◽  
Ayhan Cömert ◽  
İbrahim Tekdemir
Keyword(s):  
Skin Flaps ◽  
Dorsal Skin

A Model for Training in Elevation of Local Skin Flaps in the Hand

1994 ◽  
Vol 19 (6) ◽  
pp. 794-795 ◽  
Author(s):  
H. D. SKOFF
Keyword(s):  
Skin Flap ◽  
Three Dimensional ◽  
Skin Flaps ◽  
Dimensional Model ◽  
Surgical Experience ◽  
Three Dimensional Model ◽  
Local Skin ◽  
Practice Models ◽  
Flap Design

Appropriate skin flap design is crucial for success in surgery of the hand. Practice models are useful in acquiring surgical experience. The author describes a three-dimensional model composed of readily available materials which has been useful for practice in elevation of local skin flaps in the hand

scholarly journals Ultrastructural study of the effects of cyclosporine in the brainstem of Wistar rats submitted to the ethidium bromide demyelinating model

2008 ◽  
Vol 66 (2b) ◽  
pp. 378-384 ◽  
Author(s):  
Eduardo Fernandes Bondan ◽  
Maria Anete Lallo ◽  
Dominguita Lühers Graça
Keyword(s):  
Ethidium Bromide ◽  
Tissue Repair ◽  
Wistar Rats ◽  
Saline Solution ◽  
Immunosuppressive Treatment ◽  
Post Injection ◽  
Group I ◽  
Transmission Electron ◽  
Myelin Debris ◽  
Intracisternal Injection

The ethidium bromide-demyelinating model (EB) was used to study remyelination in the brainstem under the use of cyclosporine (CsA). Wistar rats were submitted to intracisternal injection of 0.1% EB or 0.9% saline solution, and others were taken as histologic controls (group I). Within those injected with EB, some have not received immunosuppressive treatment (II); some were treated by intraperitonial route with CsA (III.E - 10 mg/kg/day). Rats from group III.C were injected with saline solution and treated with CsA. The animals were perfused from 15 to 31 days post-injection collecting brainstem sections for light and transmission electron microscopy studies. After EB injection it was noted the presence of macrophages and non-degraded myelin debris, demyelinated axons, oligodendrocyte or Schwann cell remyelinated axons, groups of infiltrating pial cells, hypertrophic astrocytes and few lymphocytes. Tissue repair of EB-induced lesions in group III.E was similar to that of group II, but with the presence of a higher density of oligodendrocytes near remyelinating areas.

Sign in / Sign up

Export Citation Format

Share Document