An animal model for the analysis of cochlear blood flow disturbance and hearing threshold in vivo

Noise exposure affects the organ of Corti and the lateral wall of the cochlea, including the stria vascularis and spiral ligament. Although the inner ear vasculature and spiral ligament fibrocytes in the lateral wall consist of a significant proportion of cells in the cochlea, relatively little is known regarding their functional significance. In this study, 6-week-old male C57BL/6 mice were exposed to noise trauma to induce transient hearing threshold shift (TTS) or permanent hearing threshold shift (PTS). Compared to mice with TTS, mice with PTS exhibited lower cochlear blood flow and lower vessel diameter in the stria vascularis, accompanied by reduced expression levels of genes involved in vasodilation and increased expression levels of genes related to vasoconstriction. Ultrastructural analyses by transmission electron microscopy revealed that the stria vascularis and spiral ligament fibrocytes were more damaged by PTS than by TTS. Moreover, mice with PTS expressed significantly higher levels of proinflammatory cytokines in the cochlea (e.g., IL-1β, IL-6, and TNF-α). Overall, our findings suggest that cochlear microcirculation and lateral wall pathologies are differentially modulated by the severity of acoustic trauma and are associated with changes in vasoactive factors and inflammatory responses in the cochlea.

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The effect of prostaglandin E1 on brainstem blood flow disturbance in an animal model of vertebrobasilar insufficiency

European Archives of Oto-Rhino-Laryngology ◽

10.1007/s00405-013-2489-x ◽

2013 ◽

Vol 271 (5) ◽

pp. 947-951 ◽

Cited By ~ 3

Author(s):

Toshiaki Yamanaka ◽

Takayuki Murai ◽

Yachiyo Sawai ◽

Hideyuki Okamoto ◽

Hiroshi Hosoi

Keyword(s):

Animal Model ◽

Blood Flow ◽

Prostaglandin E1 ◽

Vertebrobasilar Insufficiency ◽

Flow Disturbance

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In Vivo Imaging of Mammalian Cochlear Blood Flow Using Fluorescence Microendoscopy

Otology & Neurotology ◽

10.1097/01.mao.0000190708.44067.b0 ◽

2006 ◽

Vol 27 (2) ◽

pp. 144-152 ◽

Cited By ~ 27

Author(s):

Ashkan Monfared ◽

Nikolas H Blevins ◽

Eunice L. M Cheung ◽

Juergen C Jung ◽

Gerald Popelka ◽

...

Keyword(s):

Blood Flow ◽

In Vivo Imaging ◽

Cochlear Blood Flow

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Early Surgical Decompression Restores Neurovascular Blood Flow and Ischemic Parameters in an in Vivo Animal Model of Nerve Compression Injury

The Journal of Bone and Joint Surgery (American) ◽

10.2106/jbjs.m.01116 ◽

2014 ◽

Vol 96 (11) ◽

pp. 897-906 ◽

Cited By ~ 16

Author(s):

James Jung ◽

Peter Hahn ◽

Bernard Choi ◽

Tahseen Mozaffar ◽

Ranjan Gupta

Keyword(s):

Animal Model ◽

Blood Flow ◽

Surgical Decompression ◽

Nerve Compression ◽

Compression Injury ◽

In Vivo Animal Model ◽

Nerve Compression Injury

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Microvascular features that suggest effectors of blood-flow regulation in the brain: Evidence by SEM of corrosion casts of intracerebral vessels

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158923 ◽

1990 ◽

Vol 48 (3) ◽

pp. 274-275

Author(s):

Enrico D.F. Motti ◽

Hans-Georg Imhof ◽

Gazi M. Yasargil

Keyword(s):

Blood Flow ◽

Perfusion Pressure ◽

Corrosion Casts ◽

Cerebral Microcirculation ◽

Pial Arteries ◽

Random Occurrence ◽

Brain Arteries ◽

Homogeneous Network ◽

The Brain

Physiologists have devoted most attention in the cerebrovascular tree to the arterial side of the circulation which has been subdivided in three levels: 1) major brain arteries which keep microcirculation constant despite changes in perfusion pressure; 2) pial arteries supposed to be effectors regulating microcirculation; 3) intracerebral arteries supposed to be deprived of active cerebral blood flow regulating devices.The morphological search for microvascular effectors in the cerebrovascular bed has been elusive. The opaque substance of the brain confines in vivo investigation to the superficial pial arteries. Most morphologists had to limit their observation to the random occurrence of a favorable site in the practically two-dimensional thickness of diaphanized histological sections. It is then not surprising most investigators of the cerebral microcirculation refer to an homogeneous network of microvessels interposed between arterioles and venules.We have taken advantage of the excellent depth of focus afforded by the scanning electron microscope (SEM) to investigate corrosion casts obtained injecting a range of experimental animals with a modified Batson's acrylic mixture.

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