Importance of transient receptor potential vanilloid 4 (TRPV4) in epidermal barrier function in human skin keratinocytes

2012 ◽  
Vol 463 (5) ◽  
pp. 715-725 ◽  
Author(s):  
Naoko Kida ◽  
Takaaki Sokabe ◽  
Makiko Kashio ◽  
Kunitaka Haruna ◽  
Yuki Mizuno ◽  
...  
Keyword(s):  
Human Skin ◽  
Barrier Function ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Transient Receptor Potential Vanilloid ◽  
Epidermal Barrier ◽  
Skin Keratinocytes ◽  
Transient Receptor

scholarly journals The degradation of airway tight junction protein under acidic conditions is probably mediated by transient receptor potential vanilloid 1 receptor

2013 ◽  
Vol 33 (5) ◽  
Author(s):  
Rui Xu ◽  
Qi Li ◽  
Jia Zhou ◽  
Xiang-dong Zhou ◽  
Juliy M. Perelman ◽  
...  
Keyword(s):  
Barrier Function ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Immunofluorescent Staining ◽  
Epithelial Barrier ◽  
Transient Receptor Potential Vanilloid ◽  
Airway Epithelial ◽  
Acidic Stress ◽  
Epithelial Barrier Function ◽  
Transient Receptor

Acidic airway microenvironment is one of the representative pathophysiological features of chronic inflammatory respiratory diseases. Epithelial barrier function is maintained by TJs (tight junctions), which act as the first physical barrier against the inhaled substances and pathogens of airway. As previous studies described, acid stress caused impaired epithelial barriers and led the hyperpermeability of epithelium. However, the specific mechanism is still unclear. We have showed previously the existence of TRPV (transient receptor potential vanilloid) 1 channel in airway epithelium, as well as its activation by acidic stress in 16HBE cells. In this study, we explored the acidic stress on airway barrier function and TJ proteins in vitro with 16HBE cell lines. Airway epithelial barrier function was determined by measuring by TER (trans-epithelial electrical resistance). TJ-related protein [claudin-1, claudin-3, claudin-4, claudin-5, claudin-7 and ZO-1 (zonula occluden 1)] expression was examined by western blotting of insoluble fractions of cell extraction. The localization of TJ proteins were visualized by immunofluorescent staining. Interestingly, stimulation by pH 6.0 for 8 h slightly increased the epithelial resistance in 16HBE cells insignificantly. However, higher concentration of hydrochloric acid (lower than pH 5.0) did reduce the airway epithelial TER of 16HBE cells. The decline of epithelial barrier function induced by acidic stress exhibited a TRPV1-[Ca2+]i-dependent pathway. Of the TJ proteins, claudin-3 and claudin-4 seemed to be sensitive to acidic stress. The degradation of claudin-3 and claudin-4 induced by acidic stress could be attenuated by the specific TRPV1 blocker or intracellular Ca2+ chelator BAPTA/AM [1,2-bis-(o-aminophenoxy)ethane-N,N,N',N'-tetra-acetic acid tetrakis(acetoxymethyl ester)].

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