Isolation and characterization of the 5´-upstream region of the human voltage-gated Ca2+ channel α2δ-1 auxiliary subunit gene: promoter analysis and regulation by transcription factor Sp1

2012 ◽  
Vol 465 (6) ◽  
pp. 819-828 ◽  
Author(s):  
Elizabeth Martínez-Hernández ◽  
Ricardo González-Ramírez ◽  
Alejandro Sandoval ◽  
Bulmaro Cisneros ◽  
Rodolfo Delgado-Lezama ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Promoter Analysis ◽  
Gene Promoter ◽  
Upstream Region ◽  
Subunit Gene ◽  
Voltage Gated ◽  
Isolation And Characterization ◽  
Auxiliary Subunit ◽  
Transcription Factor Sp1

scholarly journals Isolation and characterization of rubisco small subunit gene promoter from common wheat (Triticum aestivumL.)

2015 ◽  
Vol 10 (2) ◽  
pp. e989033 ◽  
Author(s):  
Shalini Mukherjee ◽  
Claudio Stasolla ◽  
Anita Brûlé-Babel ◽  
Belay T Ayele
Keyword(s):  
Common Wheat ◽  
Gene Promoter ◽  
Small Subunit ◽  
Subunit Gene ◽  
Isolation And Characterization ◽  
Rubisco Small Subunit

Characterization of the human SLC22A18 gene promoter and its regulation by the transcription factor Sp1

Gene ◽  
2009 ◽  
Vol 429 (1-2) ◽  
pp. 37-43 ◽  
Author(s):  
Abdullah Mahmood Ali ◽  
Vineeta Bajaj ◽  
K.S. Gopinath ◽  
Arun Kumar
Keyword(s):  
Transcription Factor ◽  
Gene Promoter ◽  
Transcription Factor Sp1

scholarly journals Analysis of the rat lactate dehydrogenase A subunit gene promoter/regulatory region

1994 ◽  
Vol 304 (2) ◽  
pp. 391-398 ◽  
Author(s):  
M L Short ◽  
D Huang ◽  
D M Milkowski ◽  
S Short ◽  
K Kunstman ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Lactate Dehydrogenase ◽  
Cyclic Amp ◽  
Binding Sites ◽  
Transcriptional Activity ◽  
Gene Promoter ◽  
Upstream Region ◽  
Subunit Gene ◽  
Promoter Fragment ◽  
A Subunit

The rat lactate dehydrogenase (LDH) A subunit gene promoter contains a putative AP-1 binding site at -295/-289 bp, two consensus Sp1 binding sites at -141/-136 bp and -103/-98 bp, and a single copy of a consensus cyclic AMP-responsive element (CRE) at -48 to -41 bp upstream of the transcription initiation site. Additionally, an as yet unidentified silencer element is located within the -1173/-830 bp 5′-flanking region. Transient transfection analyses of a -1173/+25 bp LDH A-chLoramphenicol acetyltransferase fusion gene has indicated a complete inability of the promoter fragment to direct basal or forskolin-induced transcription. Deletion of the -1173/-830 bp sequence restored basal and cyclic AMP (cAMP)-inducible activity. Point mutations in the Sp1 binding sites of a -830/+25 bp promoter fragment reduced basal but not the relative degree of cAMP-inducible activity. cAMP-regulated transcriptional activity was dependent upon an 8 bp CRE, -TGACGTCA-, located at the -48/-41 bp upstream region. Mutations in the CRE abolished cAMP-mediated induction and reduced basal activity by about 65%. The CRE binds a 47 kDa protein which has previously been identified as CRE binding protein (CREB)-327, an isoform of the activating transcription factor/CREB transcription factor gene family. Co-transfection of a vector that expresses the catalytic subunit of cAMP-dependent protein kinase stimulates LDH A subunit promoter activity suggesting that cAMP induces LDH A subunit gene expression through phosphorylative modification of CREB-327. This study emphasizes a fundamental role of several modules including Sp1 and CREB binding sites in regulating basal and cAMP-mediated transcriptional activity of the LDH A gene.

Characterization of the human α9 integrin subunit gene: Promoter analysis and transcriptional regulation in ocular cells

2015 ◽  
Vol 135 ◽  
pp. 146-163 ◽  
Author(s):  
Céline Duval ◽  
Karine Zaniolo ◽  
Steeve Leclerc ◽  
Christian Salesse ◽  
Sylvain L. Guérin
Keyword(s):  
Transcriptional Regulation ◽  
Promoter Analysis ◽  
Gene Promoter ◽  
Integrin Subunit ◽  
Subunit Gene

Isolation and Characterization of the Cold-Induced Phyllostachys edulis AP2/ERF Family Transcription Factor, peDREB1

2011 ◽  
Vol 30 (3) ◽  
pp. 679-689 ◽  
Author(s):  
Lei Liu ◽  
Xiao-Lu Cao ◽  
Rui Bai ◽  
Na Yao ◽  
Lu-Bin Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Phyllostachys Edulis ◽  
Isolation And Characterization ◽  
Erf Family

Isolation and characterization of an AP2/ERF-type drought stress inducible transcription factor encoding gene from rice

2013 ◽  
Vol 23 (1) ◽  
pp. 42-51 ◽  
Author(s):  
Ibandalin Mawlong ◽  
Kishwar Ali ◽  
Devika Kurup ◽  
Sangita Yadav ◽  
Aruna Tyagi
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Isolation And Characterization ◽  
Encoding Gene

scholarly journals Isolation and characterization of maize ZmPP2C26 gene promoter in drought-response

2020 ◽  
Vol 26 (11) ◽  
pp. 2189-2197
Author(s):  
Fengzhong Lu ◽  
Kexin Wang ◽  
Lamei Yan ◽  
Yalin Peng ◽  
Jingtao Qu ◽  
...  
Keyword(s):  
Gene Promoter ◽  
Drought Response ◽  
Isolation And Characterization
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