The cellular mechanisms of Cl - secretion induced by C-type natriuretic peptide (CNP). Experiments on isolated in vitro perfused rectal gland tubules of Squalus acanthias

1999 ◽  
Vol 438 (1) ◽  
pp. 15-22 ◽  
Author(s):  
R. Greger ◽  
Markus Bleich ◽  
Richard Warth ◽  
I. Thiele ◽  
John N. Forrest
Keyword(s):  
Natriuretic Peptide ◽  
Squalus Acanthias ◽  
Rectal Gland ◽  
Cellular Mechanisms ◽  
Cl Secretion

Mechanism of active chloride secretion by shark rectal gland: role of Na-K-ATPase in chloride transport

1977 ◽  
Vol 233 (4) ◽  
pp. F298-F306 ◽  
Author(s):  
P. Silva ◽  
J. Stoff ◽  
M. Field ◽  
L. Fine ◽  
J. N. Forrest ◽  
...  
Keyword(s):  
Chloride Transport ◽  
Sodium Concentration ◽  
Chloride Secretion ◽  
Squalus Acanthias ◽  
Rectal Gland ◽  
Tentative Hypothesis ◽  
Shark Rectal Gland ◽  
Electrochemical Equilibrium

The isolated rectal gland of Squalus acanthias was stimulated to secrete chloride against an electrical and a chemical gradient when perfused in vitro by theophylline and/or dibutyryl cyclic AMP. Chloride secretion was depressed by ouabain which inhibits Na-K-ATPase. Thiocyanate and furosemide also inhibited chloride secretion but ethoxzolamide, a carbonic anhydrase inhibitor, did not. Chloride transport was highly dependent on sodium concentration in the perfusate. The intracellular concentration of chloride averaged 70-80 meq/liter in intact glands, exceeding the level expected at electrochemical equilibrium and suggesting active transport of chloride into the cell. These features suggest a tentative hypothesis for chloride secretion by the rectal gland in which the uphill transport of chloride into the cytoplasm is coupled through a membrane carrier to the downhill movement of sodium along its electrochemical gradient. The latter is maintained by the Na-K-ATPase pump while chloride is extruded into the duct by electrical forces.

pH-regulatory mechanisms in in vitro perfused rectal gland tubules of Squalus acanthias

1998 ◽  
Vol 436 (2) ◽  
pp. 248-254 ◽  
Author(s):  
M. Bleich ◽  
R. Warth ◽  
I. Thiele ◽  
R. Greger
Keyword(s):  
Squalus Acanthias ◽  
Regulatory Mechanisms ◽  
Rectal Gland

Regulation of the Na + 2Cl - K + cotransporter in in vitro perfused rectal gland tubules of Squalus acanthias

1998 ◽  
Vol 436 (4) ◽  
pp. 521-528 ◽  
Author(s):  
R. Warth ◽  
M. Bleich ◽  
I. Thiele ◽  
F. Lang ◽  
R. Greger
Keyword(s):  
Squalus Acanthias ◽  
Rectal Gland

Osmotically Induced Conductance and Capacitance Changes in in vitro Perfused Rectal Gland Tubules of Squalus acanthias

1998 ◽  
Vol 21 (5) ◽  
pp. 317-324 ◽  
Author(s):  
I. Thiele ◽  
R. Warth ◽  
M. Bleich ◽  
S. Waldegger ◽  
F. Lang ◽  
...  
Keyword(s):  
Squalus Acanthias ◽  
Rectal Gland

C-type natriuretic peptides stimulate chloride secretion in the rectal gland of Squalus acanthias

1992 ◽  
Vol 262 (4) ◽  
pp. R707-R711 ◽  
Author(s):  
R. Solomon ◽  
A. Protter ◽  
G. McEnroe ◽  
J. G. Porter ◽  
P. Silva
Keyword(s):  
Natriuretic Peptide ◽  
Response Curve ◽  
Chloride Secretion ◽  
Squalus Acanthias ◽  
Constant Infusion ◽  
Rectal Gland ◽  
Ligand Effects ◽  
Physiological Regulator ◽  
Gland Function ◽  
Stimulation Of

Homologous shark C-type natriuretic peptide (sCNP) was infused as a bolus and as a constant infusion in the isolated perfused rectal gland of the same species, Squalus acanthias. sCNP was a potent stimulator of chloride secretion similar in its dose-response curve to vasoactive intestinal peptide. sCNP was equipotent with killifish CNP but more potent than human CNP (hCNP). Truncated and substituted, forms of hCNP were also capable of stimulation of chloride secretion in the order hCNP greater than hCNP (6-22) = [Gly9]hCNP greater than hCNP-(7-21). sCNP was more potent than human atrial natriuretic peptide (hANP), which was more potent than porcine brain natriuretic peptide. hANP-(31-67) was without effect. These studies suggest that sCNP may be the physiological regulator of rectal gland function. The receptor in the rectal gland is unknown but based on the order of potencies, position 4 in the NH2-terminal end and the ring itself are important for ligand effects.

The effect of organotin compounds on chloride secretion by the in vitro perfused rectal gland of Squalus acanthias

1989 ◽  
Vol 100 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Richard Solomon ◽  
Stephanie Lear ◽  
Robert Cohen ◽  
Kate Spokes ◽  
Patricio Silva ◽  
...  
Keyword(s):  
Chloride Secretion ◽  
Squalus Acanthias ◽  
Organotin Compounds ◽  
Rectal Gland
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