The role of cytosolic Ca 2+ in the secretion of NaCl in isolated in vitro perfused rectal gland tubules of Squalus acanthias

1998 ◽  
Vol 436 (1) ◽  
pp. 133-140 ◽  
Author(s):  
R. Warth ◽  
I. Thiele ◽  
M. Bleich ◽  
R. Greger
Keyword(s):  
Squalus Acanthias ◽  
Rectal Gland

Mechanism of active chloride secretion by shark rectal gland: role of Na-K-ATPase in chloride transport

1977 ◽  
Vol 233 (4) ◽  
pp. F298-F306 ◽  
Author(s):  
P. Silva ◽  
J. Stoff ◽  
M. Field ◽  
L. Fine ◽  
J. N. Forrest ◽  
...  
Keyword(s):  
Chloride Transport ◽  
Sodium Concentration ◽  
Chloride Secretion ◽  
Squalus Acanthias ◽  
Rectal Gland ◽  
Tentative Hypothesis ◽  
Shark Rectal Gland ◽  
Electrochemical Equilibrium

The isolated rectal gland of Squalus acanthias was stimulated to secrete chloride against an electrical and a chemical gradient when perfused in vitro by theophylline and/or dibutyryl cyclic AMP. Chloride secretion was depressed by ouabain which inhibits Na-K-ATPase. Thiocyanate and furosemide also inhibited chloride secretion but ethoxzolamide, a carbonic anhydrase inhibitor, did not. Chloride transport was highly dependent on sodium concentration in the perfusate. The intracellular concentration of chloride averaged 70-80 meq/liter in intact glands, exceeding the level expected at electrochemical equilibrium and suggesting active transport of chloride into the cell. These features suggest a tentative hypothesis for chloride secretion by the rectal gland in which the uphill transport of chloride into the cytoplasm is coupled through a membrane carrier to the downhill movement of sodium along its electrochemical gradient. The latter is maintained by the Na-K-ATPase pump while chloride is extruded into the duct by electrical forces.

pCMBS-induced swelling of dogfish (Squalus acanthias) rectal gland cells: role of the Na+,K+-ATPase and the cytoskeleton

1990 ◽  
Vol 1025 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Arnost Kleinzeller ◽  
George W. Booz ◽  
John W. Mills ◽  
Fuad N. Ziyadeh
Keyword(s):  
Squalus Acanthias ◽  
Rectal Gland ◽  
Gland Cells

pH-regulatory mechanisms in in vitro perfused rectal gland tubules of Squalus acanthias

1998 ◽  
Vol 436 (2) ◽  
pp. 248-254 ◽  
Author(s):  
M. Bleich ◽  
R. Warth ◽  
I. Thiele ◽  
R. Greger
Keyword(s):  
Squalus Acanthias ◽  
Regulatory Mechanisms ◽  
Rectal Gland

Regulation of the Na + 2Cl - K + cotransporter in in vitro perfused rectal gland tubules of Squalus acanthias

1998 ◽  
Vol 436 (4) ◽  
pp. 521-528 ◽  
Author(s):  
R. Warth ◽  
M. Bleich ◽  
I. Thiele ◽  
F. Lang ◽  
R. Greger
Keyword(s):  
Squalus Acanthias ◽  
Rectal Gland

Osmotically Induced Conductance and Capacitance Changes in in vitro Perfused Rectal Gland Tubules of Squalus acanthias

1998 ◽  
Vol 21 (5) ◽  
pp. 317-324 ◽  
Author(s):  
I. Thiele ◽  
R. Warth ◽  
M. Bleich ◽  
S. Waldegger ◽  
F. Lang ◽  
...  
Keyword(s):  
Squalus Acanthias ◽  
Rectal Gland

The effect of organotin compounds on chloride secretion by the in vitro perfused rectal gland of Squalus acanthias

1989 ◽  
Vol 100 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Richard Solomon ◽  
Stephanie Lear ◽  
Robert Cohen ◽  
Kate Spokes ◽  
Patricio Silva ◽  
...  
Keyword(s):  
Chloride Secretion ◽  
Squalus Acanthias ◽  
Organotin Compounds ◽  
Rectal Gland

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

An integrated screening method for evaluating the pulmonary toxicity of inhaled particulates: Role of microscopic techniques in assessing pulmonary macrophage clearance functions

1990 ◽  
Vol 48 (3) ◽  
pp. 826-827
Author(s):  
David B. Warheit ◽  
Lena Achinko ◽  
Mark A. Hartsky
Keyword(s):  
Bronchoalveolar Lavage ◽  
Pulmonary Toxicity ◽  
Screening Method ◽  
Pulmonary Response ◽  
Inhaled Particles ◽  
Cytochemical Analysis ◽  
Pulmonary Macrophages ◽  
Integrated Screening

There is a great need for the development of a rapid and reliable bioassay to evaluate the pulmonary toxicity of inhaled particles. A number of methods have been proposed, including lung clearance studies, bronchoalveolar lavage analysis, and in vitro cytotoxicity tests. These methods are often limited in scope inasmuch as they measure only one dimension of the pulmonary response to inhaled, instilled or incubated dusts. Accordingly, a comprehensive approach to lung toxicity studies has been developed.To validate the method, rats were exposed for 6 hours or 3 days to various concentrations of either aerosolized alpha quartz silica (Si) or carbonyl iron (CI) particles. Cells and fluids from groups of sham and dust-exposed animals were recovered by bronchoalveolar lavage (BAL). Alkaline phosphatase, LDH and protein values were measured in BAL fluids at several time points postexposure. Cells were counted and evaluated for viability, as well as differential and cytochemical analysis. In addition, pulmonary macrophages (PM) were cultured and studied for morphology, chemotaxis, and phagocytosis by scanning electron microscopy.

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