Molecular and physiological characterisation of a 14-3-3 protein from lily pollen grains regulating the activity of the plasma membrane H + ATPase during pollen grain germination and tube growth

Planta ◽  
2001 ◽  
Vol 213 (1) ◽  
pp. 132-141 ◽  
Author(s):  
Heidi Pertl ◽  
Martin Himly ◽  
Renate Gehwolf ◽  
Rosa Kriechbaumer ◽  
Doris Strasser ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Growth ◽  
Pollen Grain Germination ◽  
Lily Pollen

scholarly journals Pollen tube incompatibility reaction on the stigma in selfpollinated Sinapis alba L.

2014 ◽  
Vol 65 (1-2) ◽  
pp. 101-105 ◽  
Author(s):  
Renata Śnieżko ◽  
Krystyna Winiarczyk
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Grains ◽  
Pollen Tubes ◽  
Sinapis Alba ◽  
Pollen Grain ◽  
Tube Growth ◽  
Sinapis Alba L ◽  
Growth Changes ◽  
Incompatibility Reaction

After selfpollination of <em>Sinapis alba</em> L. pollen tubes growth is inhibited on the stigma. The pollen grains germinate 3-4 hours after pollination. The pollen give rise to one or more pollen tubes. They grow along the papillae. In the place of contact between the papilla and pollen tube the pellicula is digested. Then the direction of pollen tube growth changes completely. Pollen tubes grow back on the exine of their own pollen grain, or turn into the air. The pollen tubes growth was inhibited in 6-8 hours after selfpollination. After crosspollination usually there is no incompatibility reaction.

scholarly journals In vitro Pollen Grain Germination and Tube Growth of Ten Iranian Jujube (Ziziphus jujuba) Cultivars

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Hossein ROUHAKHSH ◽  
Gholamhossein DAVARYNEJAD ◽  
Majid RAHEMI ◽  
Bahram ABEDI
Keyword(s):  
Pollen Grain ◽  
Tube Growth ◽  
Pollen Grain Germination ◽  
Ziziphus Jujuba

scholarly journals Pollen grain germination and fruit set in 'Brazilian seedless' sugar apple (Annona squamosa L.)

2012 ◽  
Vol 12 (4) ◽  
pp. 277-280 ◽  
Author(s):  
Hellenn Thallyta Alves Mendes ◽  
Márcia Regina Costa ◽  
Silvia Nietsche ◽  
João Alison Alves Oliveira ◽  
Marlon Cristian Toledo Pereira
Keyword(s):  
Fruit Set ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Pollen Grain Germination ◽  
Artificial Pollination ◽  
Randomized Design ◽  
Self Fertilization ◽  
Completely Randomized Design ◽  
Fruit Formation

The purpose of this study was to evaluate fruit set and pollen grain germination of 'Brazilian seedless' sugar apple. Two experiments were performed: 1) one in a completely randomized design with three treatments (natural and artificial pollination and self-fertilization), with three replications of 10 flowers per plot, 2) and the other in a completely randomized design with three treatments: pollen grains of a seeded wild-type, 'Brazilian seedless' and atemoya (hybrid of sugar apple and cherimoya) 'Gefner', with five replications. A fruit set of 100% was achieved by artificial pollination. No fruit formation was observed after self-fertilization. The percentage of pollen grain germination in vitro was highest for 'Brazilian seedless' (52.5%) and lowest for cultivar Gefner (5.9%). Preliminary results indicated that pollen grains of 'Brazilian seedless' are viable and that natural or artificial pollination is essential for fruit set.

POLLEN GERMINATION AND CHILLING SENSITIVITY IN TWO TOMATO CULTIVARS OF DIFFERING SENSITIVITY TO COOL NIGHT TEMPERATURE

1982 ◽  
Vol 62 (3) ◽  
pp. 765-770 ◽  
Author(s):  
N. P. A. HUNER ◽  
R. B. VAN HUYSTEE
Keyword(s):  
Low Temperature ◽  
Pollen Tube ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Differential Rate ◽  
Night Temperature ◽  
Pollen Grain Germination ◽  
Chilling Sensitivity ◽  
Tomato Cultivars ◽  
Cool Night

The effect of low night temperature on pollen tube formation and chilling sensitivity was compared in two tomato cultivars, Ontario 7515, free-setting at low temperature, and Ohio MR13, poor-setting at low temperature. The rate and extent of pollen grain germination in the Ontario 7515 cultivar far exceeded that of pollen grains obtained from the Ohio MR13 cultivar. In addition, the rate of pollen tube growth was greater in the free-setting cultivar than the poor-setting cultivar. No major differences were observed in the chilling sensitivity of the vegetative parts of these two tomato cultivars. It is concluded that the differential ability of these two tomato varieties to set fruit during growth at cool, night temperatures may be a function of differential flower production and a differential rate of pollen grain germination rather than a differential chilling sensitivity of the vegetative plant.

pH modulates interaction of 14-3-3 proteins with pollen plasma membrane H+ ATPases independently from phosphorylation

2021 ◽  
Author(s):  
Heidi Pertl-Obermeyer ◽  
Ana Gimeno ◽  
Verena Kuchler ◽  
Evrim Servili ◽  
Shuai Huang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Grains ◽  
Tube Growth ◽  
In Vitro Assays ◽  
Sudden Increase ◽  
Ph Gradients ◽  
Threonine Residue

Abstract Pollen grains transport the sperm cells through the style tissue via a fast-growing pollen tube to the ovaries where fertilization takes place. Pollen tube growth requires a precisely regulated network of cellular as well as molecular events including the activity of the plasma membrane H+ ATPase, which is known to be regulated by reversible protein phosphorylation and subsequent binding of 14-3-3 isoforms. Immunodetection of the phosphorylated penultimate threonine residue of the pollen plasma membrane H+ ATPase (LilHA1) of Lilium longiflorum pollen revealed a sudden increase in phosphorylation with the start of pollen tube growth. In addition to phosphorylation, pH modulated the binding of 14-3-3 isoforms to the regulatory domain of the H+ ATPase, whereas metabolic components had only small effects on 14-3-3 binding, as tested with in vitro assays using recombinant 14-3-3 isoforms and phosphomimicking substitutions of the threonine residue. Consequently, local H+ influxes and effluxes as well as pH gradients in the pollen tube tip are generated by localized regulation of the H+ ATPase activity rather than by heterogeneous localized distribution in the plasma membrane.

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