Comparative evaluation of immunization with recombinant protein and plasmid DNA vaccines of fusion antigen ROP2 and SAG1 from Toxoplasma gondii in mice: cellular and humoral immune responses

2011 ◽  
Vol 109 (3) ◽  
pp. 637-644 ◽  
Author(s):  
Wen-Shu Li ◽  
Qing-Xin Chen ◽  
Ju-Xiu Ye ◽  
Zi-Xin Xie ◽  
Jun Chen ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Recombinant Protein ◽  
Immune Responses ◽  
Plasmid Dna ◽  
Comparative Evaluation ◽  
Dna Vaccines ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Fusion Antigen

scholarly journals Experimental feline toxoplasmosis: Humoral immune responses of cats inoculated orally with Toxoplasma gondii cysts and oocysts.

1990 ◽  
Vol 52 (4) ◽  
pp. 865-867 ◽  
Author(s):  
Yoshitaka OMATA ◽  
Hiroshi OIKAWA ◽  
Masanori KANDA ◽  
Kouji MIKAZUKI ◽  
Toshio NAKABAYASHI ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immune Responses ◽  
Humoral Immune ◽  
Humoral Immune Responses

scholarly journals Humoral immune responses against members of the HSP70 family in Toxoplasma gondii

1998 ◽  
Vol 47 ◽  
pp. 272
Author(s):  
K. Yui ◽  
K Yamashita ◽  
M. Ueda ◽  
H. Udono ◽  
A. Yano
Keyword(s):  
Toxoplasma Gondii ◽  
Immune Responses ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Hsp70 Family

Humoral immune responses in chickens and turkeys after infection with Toxoplasma gondii by using recombinant antigens

2014 ◽  
Vol 113 (4) ◽  
pp. 1473-1480 ◽  
Author(s):  
Andrea Hotop ◽  
Susanne Buschtöns ◽  
Berit Bangoura ◽  
Birte Zöller ◽  
Martin Koethe ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immune Responses ◽  
Recombinant Antigens ◽  
Humoral Immune ◽  
Humoral Immune Responses

Vaccination with plasmid DNA expressing antigen from genomic or cDNA gene forms induces equivalent humoral immune responses

Vaccine ◽  
1999 ◽  
Vol 18 (7-8) ◽  
pp. 692-702 ◽  
Author(s):  
Damien R Drew ◽  
Marshall Lightowlers ◽  
Richard A Strugnell
Keyword(s):  
Immune Responses ◽  
Plasmid Dna ◽  
Humoral Immune ◽  
Humoral Immune Responses

scholarly journals Toxoplasma gondii: humoral and cellular immune response of BALB/c mice immunized via intranasal route with rTgROP2

2010 ◽  
Vol 19 (4) ◽  
pp. 210-216 ◽  
Author(s):  
Michelle Igarashi ◽  
Dauton Luiz Zulpo ◽  
Ivo Alexandre Leme da Cunha ◽  
Luiz Daniel Barros ◽  
Vanessa Figueredo Pereira ◽  
...  
Keyword(s):  
Immune Response ◽  
Recombinant Protein ◽  
Immune Responses ◽  
Stimulation Index ◽  
Intranasal Route ◽  
Iptg Induction ◽  
Humoral Immune ◽  
Cellular Immune Responses ◽  
Humoral Immune Responses ◽  
Cellular Immune

TgROP2 is an intracellular protein associated with rhoptries of Toxoplama gondii and an antigen component of a candidate vaccine for toxoplasmosis. The purpose of the present study was to evaluate the efficacy of rTgROP2 to stimulate humoral and cellular immune responses in BALB/c mice via intranasal injection. TgROP2 partial coding sequence was (196-561) amplified by PCR from genomic T. gondii RH strain DNA and cloned into the pTrcHis expression vector. Escherichia coli Rosetta 2 cells transformed with pTrcHis-TgROP2 showed high levels (~1 mg.mL-1) of recombinant protein after 4 hours of IPTG induction. Recombinant TgROP2 exhibited an apparent Mr equal to 54 kDa. In order to test immunogenicity of the recombinant protein, 10 BALB/c mice received 10 µg of rROP2 protein + 10 µg of Quil-A via intranasal injection. Doses were administered at days 0, 21, and 42. Three animals were euthanized and used to evaluate cell-ular immune response on day 62. Five (50%) and two (20%) out of ten animals produced IgG (DO mean = 0.307; cut-off = 0.240) and IgA (DO mean = 0.133, cut-off = 0.101), respectively, by ELISA on day 62. The proliferation of splenocytes revealed high stimulation index (SI) when co-cultured with 5, 10 and 15 µg.mL-1 of rTgROP2. These results indicate that intranasal immunization with recombinant protein ROP2 plus Quil-A can elicit both cellular and humoral immune responses in BALB/c mice.

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