Occurrence and molecular characterization of free-living amoeba species (Acanthamoeba, Hartmannella, and Saccamoeba limax) in various surface water resources of Iran

2015 ◽  
Vol 114 (12) ◽  
pp. 4669-4674 ◽  
Author(s):  
Mohammad Reza Mahmoudi ◽  
Behnaz Rahmati ◽  
Seyed Hosssen Seyedpour ◽  
Panagiotis Karanis
Keyword(s):  
Surface Water ◽  
Water Resources ◽  
Molecular Characterization ◽  
Free Living ◽  
Surface Water Resources ◽  
Free Living Amoeba

scholarly journals Bio-characterization of Some Free Living Amoebae in the Surface Water of the City of Yaounde: Relationship to Physico-chemical Parameters of the Medium

2018 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Kapso Tchouankep Mireille ◽  
Ajeagah Gideon Aghaindum ◽  
Nkeng George Elambo ◽  
Ngassam Pierre
Keyword(s):  
Surface Water ◽  
Suspended Solids ◽  
River System ◽  
Organic Pollution ◽  
Chemical Pollution ◽  
Free Living ◽  
Physico Chemical ◽  
Free Living Amoeba ◽  
High Pollution

A study was carried in Yaounde to evaluate the level of organic pollution in the surface water and to determine the distribution of pathogenic free living amoeba in the aquatic ecosystem. Six points were selected on the Olezoa and the Abiergue streams of Yaounde for our study. Physico-chemical pollution was carried out by standard methods of assessment. The parameters measured were suspended solids, electric conductivity, pH, TDS, Oxydability, BOD5, COD, humidity, Dissolved Oxygen. The free living amoeba were identified by direct observation on microscope and observation after culture on Non Nutritive Agar (NNA)and Page’s Agar Saline Solution (PAS) which are all enriched with Escherichia coli. The data obtained reveal a high pollution of these urban streams of the Mfoundi River system and presents a high densities of telluric amoebae such as Acanthamoeba (21 Cell./L), Balamuthia (11 Cell./L), Naegleria (6 Cell./L), Vanella (17 Cell./L) and Vermamoeba (5 Cell./L). The sizes vary between 12 to 94 μm for the different forms isolated and identified in our study. The presence of amoeba are significantly correlated with hydrometry, temperature, nitrates suspended solids, COD and BOD5 (p <0.001). These organisms in water show contamination of the groundwater by wastewater during the study period.

scholarly journals Investigating the frequency of Free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019-2020

2020 ◽  
Author(s):  
Homa Attariani ◽  
Habibollah Turki ◽  
Saeed Shoja ◽  
Abdoreza Salahi-Moghaddam ◽  
Amin Ghanbarnejad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Water Resources ◽  
Culture Method ◽  
Positive Sample ◽  
Sufficient Information ◽  
Molecular Method ◽  
Free Living ◽  
Free Living Amoeba ◽  
Bandar Abbas ◽  
The City

Abstract Objective: These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is necessary to determine various forms of organisms in water resources to prevent the danger they can cause and risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency and determine the related genotypes. Results: Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified as Acanthamoeba (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enabled us to distinguish two genotypes of T4 (7 cases) and T15 (1 case) in this study.

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