Preparation, characterization, and evaluation of eosin B–loaded nano-liposomes for growth inhibition of Plasmodium falciparum

2022 ◽  
Author(s):  
Mana Najafzadeh ◽  
Haleh Bakhshandeh ◽  
Zahra Zamani ◽  
Monire Movahedi ◽  
Seyed Mohammad Atyabi
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Eosin B

scholarly journals Stage-dependent effect of deferoxamine on growth of Plasmodium falciparum in vitro

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1250-1255 ◽  
Author(s):  
S Whitehead ◽  
TE Peto
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Antimalarial Activity ◽  
Clinical Malaria ◽  
Inhibitory Effect ◽  
Parasite Development ◽  
And Control ◽  
Speed Of Onset

Abstract Deferoxamine (DF) has antimalarial activity that can be demonstrated in vitro and in vivo. This study is designed to examine the speed of onset and stage dependency of growth inhibition by DF and to determine whether its antimalarial activity is cytostatic or cytocidal. Growth inhibition was assessed by suppression of hypoxanthine incorporation and differences in morphologic appearance between treated and control parasites. Using synchronized in vitro cultures of Plasmodium falciparum, growth inhibition by DF was detected within a single parasite cycle. Ring and nonpigmented trophozoite stages were sensitive to the inhibitory effect of DF but cytostatic antimalarial activity was suggested by evidence of parasite recovery in later cycles. However, profound growth inhibition, with no evidence of subsequent recovery, occurred when pigmented trophozoites and early schizonts were exposed to DF. At this stage in parasite development, the activity of DF was cytocidal and furthermore, the critical period of exposure may be as short as 6 hours. These observations suggest that iron chelators may have a role in the treatment of clinical malaria.

In silico evaluation and in vitro growth inhibition of Plasmodium falciparum by natural amides and synthetic analogs

2020 ◽  
Vol 119 (6) ◽  
pp. 1879-1887
Author(s):  
Minelly Azevedo da Silva ◽  
Márcia Paranho Veloso ◽  
Kassius de Souza Reis ◽  
Guilherme de Matos Passarini ◽  
Ana Paula de Azevedo dos Santos ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
In Silico ◽  
In Vitro Growth ◽  
Synthetic Analogs

scholarly journals Bliss' and Loewe's additive and synergistic effects in Plasmodium falciparum growth inhibition by AMA1-RON2L, RH5, RIPR and CyRPA antibody combinations

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yvonne Azasi ◽  
Shannon K. Gallagher ◽  
Ababacar Diouf ◽  
Rebecca A. Dabbs ◽  
Jing Jin ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Synergistic Effects

scholarly journals Human-human hybridomas secreting monoclonal antibodies to the Mr 195,000 Plasmodium falciparum blood stage antigen.

1986 ◽  
Vol 163 (1) ◽  
pp. 179-188 ◽  
Author(s):  
R Schmidt-Ullrich ◽  
J Brown ◽  
H Whittle ◽  
P S Lin
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Protective Immunity ◽  
Lymphoblastoid Cell Line ◽  
In Vitro Growth ◽  
Human Lymphoblastoid Cell ◽  
Immune Precipitation ◽  
Human Lymphoblastoid Cell Line ◽  
Donor Lymphocytes

Using the human lymphoblastoid cell line, GM 4672, and PBL of Gambian adults immune to Plasmodium falciparum (Pf) malaria, we have produced human-human hybridomas and selected those that produce mAb against Pf antigens. The fusion frequency, using PWM-stimulated donor lymphocytes was between 6.8 X 10(-5) and 1.5 X 10(-6). Using immune fluorescence, immune precipitation, and Pf in vitro growth inhibition, we cloned four hybridomas that reacted with the Pf Mr 195,000 schizont/merozoite protein. The differences in proteins immune precipitated and in growth inhibition indicate that, during development of protective immunity against Pf malaria, a spectrum of antibodies is produced reacting with different epitopes on the same antigen. Only a portion of these antibodies exhibits biological activity, suggesting that the recognition of certain epitopes is required for the development of a protective immune response.

scholarly journals Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays

2010 ◽  
Vol 9 (1) ◽  
pp. 152 ◽  
Author(s):  
Danny W Wilson ◽  
Brendan S Crabb ◽  
James G Beeson
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
In Vitro Growth

Irreversible inhibition of S-adenosylmethionine decarboxylase in Plasmodium falciparum-infected erythrocytes: Growth inhibition in vitro

1991 ◽  
Vol 41 (11) ◽  
pp. 1713-1718 ◽  
Author(s):  
Paul S. Wright ◽  
Timothy L. Byers ◽  
Doreen E. Cross-Doersen ◽  
Peter P. McCann ◽  
Alan J. Bitonti
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Adenosylmethionine Decarboxylase ◽  
Irreversible Inhibition

scholarly journals The Glutamate-Rich Protein (GLURP) of Plasmodium falciparum Is a Target for Antibody-Dependent Monocyte-Mediated Inhibition of Parasite Growth In Vitro

1998 ◽  
Vol 66 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Michael Theisen ◽  
Soe Soe ◽  
Claude Oeuvray ◽  
Alan W. Thomas ◽  
Jens Vuust ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Cross Reactivity ◽  
Parasite Growth ◽  
Biologically Active ◽  
Dependent Manner ◽  
Igg Antibodies ◽  
Inhibitory Effects ◽  
Direct Invasion

ABSTRACT Monocyte-dependent as well as direct inhibitory effects of antimalarial antibodies point toward antigens accessible at the time of merozoite release as targets for biologically active antibodies capable of mediating protection against Plasmodium falciparum. The glutamate-rich protein (GLURP), being an antigen associated with mature schizont-infected erythrocytes, was therefore the object of the present investigation, in which we analyzed whether anti-GLURP antibodies can either interfere directly with merozoite invasion or act indirectly by promoting a monocyte-dependent growth inhibition, antibody-dependent cellular inhibition. GLURP-specific human immunoglobulin G (IgG) antibodies, from pooled IgG of healthy Liberian adults who were clinically immune to malaria, were purified by affinity chromatography on columns containing R0 (N-terminal nonrepetitive region of GLURP) or R2 (C-terminal repetitive region of GLURP) recombinant protein or synthetic peptides as ligands. Analysis of the pattern of reactivity of highly purified anti-GLURP antibodies led to the definition of at least four B-cell epitopes. One epitope was specific for R0, two were specific for R2, and the fourth displayed cross-reactivity between R0 and R2. None of the purified IgG antibodies had direct invasion-inhibitory effects, even at high concentrations. In contrast, when allowed to cooperate with monocytes, all anti-GLURP IgG preparations mediated a strong monocyte-dependent parasite growth inhibition in a dose-dependent manner.

Sign in / Sign up

Export Citation Format

Share Document