Identification and characterization of novel phosphate regulon genes, ecs0540–ecs0544, in Escherichia coli O157:H7

2010 ◽  
Vol 284 (3) ◽  
pp. 197-205 ◽  
Author(s):  
Yusuke Yoshida ◽  
Shinichiro Sugiyama ◽  
Tomoya Oyamada ◽  
Katsushi Yokoyama ◽  
Kozo Makino
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Identification And Characterization ◽  
Phosphate Regulon

scholarly journals Identification and Characterization of Spontaneous Deletions within the Sp11-Sp12 Prophage Region of Escherichia coli O157:H7 Sakai

2013 ◽  
Vol 79 (6) ◽  
pp. 1934-1941 ◽  
Author(s):  
Chun Chen ◽  
Carrie R. Lewis ◽  
Kakolie Goswami ◽  
Elisabeth L. Roberts ◽  
Chitrita DebRoy ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Genomic Region ◽  
Escherichia Coli O157 ◽  
Content Type ◽  
E Coli ◽  
Food Borne ◽  
Genes Encoding ◽  
Identification And Characterization

ABSTRACTProphages make up 12% of the enterohemorrhagicEscherichia coligenome and play prominent roles in the evolution and virulence of this food-borne pathogen. Acquisition and loss of and rearrangements within prophage regions are the primary causes of differences in pulsed-field gel electrophoresis (PFGE) patterns among strains ofE. coliO157:H7. Sp11 and Sp12 are two tandemly integrated and putatively defective prophages carried byE. coliO157:H7 strain Sakai. In this study, we identified 3 classes of deletions that occur within the Sp11-Sp12 region, at a frequency of ca. 7.74 × 10−4. One deletion resulted in a precise excision of Sp11, and the other two spanned the junction of Sp11 and Sp12. All deletions resulted in shifts in the XbaI fragment pattern observed by PFGE. We sequenced the inducible prophage pool of Sakai but did not identify any mature phage particles corresponding to either Sp11 or Sp12. Deletions containingpchBandpsrC, which are Sp11-carried genes encoding proteins known or suspected to regulate type III secretion, did not affect the secretion levels of the EspA or EspB effector. Alignment of the Sp11-Sp12 DNA sequence with its corresponding regions in otherE. coliO157:H7 and O55:H7 strains suggested that homologous recombination rather than integrase-mediated excision is the mechanism behind these deletions. Therefore, this study provides a mechanism behind the previously observed genetic instability of this genomic region ofE. coliO157:H7.

scholarly journals Identification and Characterization of Novel Compounds Blocking Shiga Toxin Expression in Escherichia coli O157:H7

2016 ◽  
Vol 7 ◽  
Author(s):  
Alejandro Huerta-Uribe ◽  
Zoe R. Marjenberg ◽  
Nao Yamaguchi ◽  
Stephen Fitzgerald ◽  
James P. R. Connolly ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
Expression In Escherichia Coli ◽  
Identification And Characterization

scholarly journals Isolation and characterization of escherichia coli in ready-to-eat foods vended in Islamic University, Kushtia

1970 ◽  
Vol 18 ◽  
pp. 99-103 ◽  
Author(s):  
S Biswas ◽  
MAK Parvez ◽  
M Shafiquzzaman ◽  
S Nahar ◽  
MN Rahman
Keyword(s):  
Escherichia Coli ◽  
Pattern Analysis ◽  
University Campus ◽  
Rflp Pattern ◽  
E Coli ◽  
Fish Meat ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Identification And Characterization

Context: Escherichia coli is shed in the feces of warm blooded animals and humans and thus potential for public health. Detection and characterization of E. coli in the ready-to-eat (RTE) foods concerns due to their presence indicates fecal contamination of the food.   Objective: To identify, characterize and RFLP pattern analysis of E. coli isolated from RTE foods vended in Islamic University campus, Kushtia.   Materials and Methods: Fifty samples from four types of consumed foods in six student halls of residence, some temporary restaurants of Islamic University, Kushtia were assessed for bacterial contamination by standard methods. Identification and characterization of E. coli isolates were performed using IMViC tests. Genomic DNA was used to perform RFLP pattern analysis.   Results: Thirty seven out of 50 (74%) examined samples of RTE foods had E. coli contamination. The highest number of E. coli was isolated from vegetable oriented RTE foods (90.90%) and fish, meat and cereals samples were also significantly E. coli positive. RFLP profiling of two E. coli isolates were observed.   Conclusion: The results of this study provide evidence that some RTE foods had unsatisfactory levels of contamination with E. coli. Thus street vended RTE food could be important potential vehicles for food-borne diseases. Molecular characterization may be exploited to identify food borne pathogen among different species.  Keywords: Ready-to-eat foods; Escherichia coli; RFLP pattern DOI: http://dx.doi.org/10.3329/jbs.v18i0.8783 JBS 2010; 18(0): 99-103

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