Thyrocytes, but not C cells, actively undergo growth and folliculogenesis at the periphery of thyroid tissue fragments in three-dimensional collagen gel culture

The thyroid is composed of thyroid follicles supported by extracellular matrix, capillary network, and stromal cell types such as fibroblasts. The follicles consist of thyrocytes and C cells. In this microenvironment, thyrocytes are highly integrated in their specific structural and functional polarization, but monolayer and floating cultures cannot allow thyrocytes to organize the follicles with such polarity. In contrast, three-dimensional (3-D) collagen gel culture enables thyrocytes to form 3-D follicles with normal polarity. However, these systems never reconstruct the follicles consisting of both thyrocytes and C cells. Thyroid tissue-organotypic culture retains 3-D follicles with both thyrocytes and C cells. To create more appropriate experimental models, we here characterize four culture systems above and then introduce the models for studying thyroid biology and disorders. Finally, we propose a new approach to the cell type-specific culture systems on the basis of in vivo microenvironments of various cell types.

Download Full-text

Effects of Thalidomide, Cytochrome P-450 and TNF-.ALPHA. on Angiogenesis in a Three-dimensional Collagen Gel-culture.

Okajimas Folia Anatomica Japonica ◽

10.2535/ofaj.79.101 ◽

2002 ◽

Vol 79 (4) ◽

pp. 101-106 ◽

Cited By ~ 8

Author(s):

Keiko FUJITA ◽

Yoshiko ASAMI ◽

Eiko MURATA ◽

Masumi AKITA ◽

Katsuji KANEKO

Keyword(s):

Three Dimensional ◽

Collagen Gel ◽

Tnf Alpha ◽

Collagen Gel Culture ◽

Cytochrome P 450 ◽

Gel Culture

Download Full-text

Two types of three-dimensional structures formed by epithelial cell aggregates from the rat ventral prostate in collagen gel culture. The amount of activated charcoal for absorbing serum

Annals of Anatomy - Anatomischer Anzeiger ◽

10.1016/s0940-9602(98)80081-2 ◽

1998 ◽

Vol 180 (3) ◽

pp. 247-251

Author(s):

Hideto Kawamura ◽

Ichiro Ichihara

Keyword(s):

Epithelial Cell ◽

Activated Charcoal ◽

Three Dimensional ◽

Collagen Gel ◽

Ventral Prostate ◽

Cell Aggregates ◽

Collagen Gel Culture ◽

Rat Ventral Prostate ◽

Gel Culture

Download Full-text

Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture

Stem Cells International ◽

10.1155/2013/927403 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Masumi Akita ◽

Kayoko Tanaka ◽

Sachiko Matsumoto ◽

Kumiko Komatsu ◽

Keiko Fujita

Keyword(s):

Progenitor Cell ◽

Three Dimensional ◽

Collagen Gel ◽

Cell Marker ◽

Capillary Tubes ◽

Antiangiogenic Effect ◽

Hematopoietic Stem ◽

Collagen Gel Culture ◽

Aortic Explants ◽

Gel Culture

We detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the capacity to form capillary tubes. Lovastatin strongly inhibited cell migration from aortic explants and caused the degradation of the capillary tubes. The present study provides insight into the function of CD133 during angiogenesis as well as an explanation for the antiangiogenic effect of statins.

Download Full-text