Ultrastructural correlates of the antidiuretic hormone-dependent and antidiuretic hormone-independent increase of osmotic water permeability in the frog urinary bladder epithelium

1998 ◽  
Vol 293 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Y. Y. Komissarchik ◽  
E. S. Snigirevskaya ◽  
E. I. Shakhmatova ◽  
Y. V. Natochin
Keyword(s):  
Urinary Bladder ◽  
Antidiuretic Hormone ◽  
Water Permeability ◽  
Osmotic Water Permeability ◽  
Frog Urinary Bladder ◽  
Bladder Epithelium ◽  
Osmotic Water

AVP-independent high osmotic water permeability of frog urinary bladder and autacoids

1996 ◽  
Vol 433 (1-2) ◽  
pp. 136-145 ◽  
Author(s):  
Y. V. Natochin ◽  
R. G. Parnova ◽  
E. I. Shakhmatova ◽  
Y. Y. Komissarchik ◽  
M. S. Brudnaya ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Water Permeability ◽  
Osmotic Water Permeability ◽  
Frog Urinary Bladder ◽  
Osmotic Water

Prostaglandin E2 inhibits vasotocin-induced osmotic water permeability in the frog urinary bladder by EP1-receptor-mediated activation of NO/cGMP pathway

2007 ◽  
Vol 293 (1) ◽  
pp. R528-R537 ◽  
Author(s):  
Vera Bachteeva ◽  
Ekaterina Fock ◽  
Elena Lavrova ◽  
Svetlana Nikolaeva ◽  
Stepan Gambaryan ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Urinary Bladder ◽  
Water Permeability ◽  
Inhibitory Effect ◽  
No Synthase ◽  
Arginine Vasotocin ◽  
Osmotic Water Permeability ◽  
Frog Urinary Bladder ◽  
Osmotic Water ◽  
Cgmp Pathway

PGE2 is a well-known inhibitor of the antidiuretic hormone-induced increase of osmotic water permeability (OWP) in different osmoregulatory epithelia; however, the mechanisms underlying this effect of PGE2 are not completely understood. Here, we report that, in the frog Rana temporaria urinary bladder, EP1-receptor-mediated inhibition of arginine-vasotocin (AVT)-induced OWP by PGE2 is attributed to increased generation of nitric oxide (NO) in epithelial cells. It was shown that the inhibitory effect of 17-phenyl-trinor-PGE2 (17-ph-PGE2), an EP1 agonist, on AVT-induced OWP was significantly reduced in the presence of 7-nitroindazole (7-NI), a neuronal NO synthase (nNOS) inhibitor. NO synthase (NOS) activity in both lysed and intact epithelial cells measured as a rate of conversion of l-[3H]arginine to l-[3H]citrulline was Ca2+ dependent and inhibited by 7-NI. PGE2 and 17-ph-PGE2, but not M&B-28767 (EP3 agonist) or butaprost (EP2 agonist), stimulated NOS activity in epithelial cells. The above effect of PGE2 was abolished in the presence of SC-19220, an EP1 antagonist. 7-NI reduced the stimulatory effect of 17-ph-PGE2 on NOS activity. 17-ph-PGE2 increased intracellular Ca2+ concentration and cGMP in epithelial cells. Western blot analysis revealed an nNOS expression in epithelial cells. These results show that the inhibitory effect of PGE2 on AVT-induced OWP in the frog urinary bladder is based at least partly on EP1-receptor-mediated activation of the NO/cGMP pathway, suggesting a novel cross talk between AVT, PGE2, and nNOS that may be important in the regulation of water transport.

scholarly journals Very high water permeability in vasopressin-induced endocytic vesicles from toad urinary bladder.

1989 ◽  
Vol 94 (6) ◽  
pp. 1101-1115 ◽  
Author(s):  
L B Shi ◽  
A S Verkman
Keyword(s):  
Urinary Bladder ◽  
Water Permeability ◽  
Time Course ◽  
Toad Urinary Bladder ◽  
Stopped Flow ◽  
Osmotic Water Permeability ◽  
Luminal Membrane ◽  
Osmotic Water ◽  
Group A ◽  
Endocytic Vesicles

The regulation of transepithelial water permeability in toad urinary bladder is believed to involve a cycling of endocytic vesicles containing water transporters between an intracellular compartment and the cell luminal membrane. Endocytic vesicles arising from luminal membrane were labeled selectively in the intact toad bladder with the impermeant fluid-phase markers 6-carboxyfluorescein (6CF) or fluorescein-dextran. A microsomal preparation containing labeled endocytic vesicles was prepared by cell scraping, homogenization, and differential centrifugation. Osmotic water permeability was measured by a stopped-flow fluorescence technique in which microsomes containing 50 mM mannitol, 5 mM K phosphate, pH 8.5 were subject to a 60-mM inwardly directed gradient of sucrose; the time course of endosome volume, representing osmotic water transport, was inferred from the time course of fluorescence self-quenching. Endocytic vesicles were prepared from toad bladders with hypoosmotic lumen solution treated with (group A) or without (group B) serosal vasopressin at 23 degrees C, and bladders in which endocytosis was inhibited by treatment with vasopressin at 0-2 degrees C (group C), or with vasopressin plus sodium azide at 23 degrees C (group D). Stopped-flow results in all four groups showed a slow rate of 6CF fluorescence decrease (time constants 1.0-1.7 s for exponential fit) indicating a component of nonendocytic 6CF entrapment into sealed vesicles. However, in vesicles from group A only, there was a very rapid 6CF fluorescence decrease (time constant 9.6 +/- 0.2 ms, SEM, 18 separate preparations) with an osmotic water permeability coefficient (Pf) of greater than 0.1 cm/s (18 degrees C) and activation energy of 3.9 +/- 0.8 kcal/mol (16 kJ/mol). Pf was inhibited reversibly by greater than 60% by 1 mM HgCl2. The rapid fluorescence decrease was absent in vesicles in groups B, C, and D. These results demonstrate the presence of functional water transporters in vasopressin-induced endocytic vesicles from toad bladder, supporting the hypothesis that water channels are cycled to and from the luminal membrane and providing a functional marker for the vasopressin-sensitive water channel. The calculated Pf in the vasopressin-induced endocytic vesicles is the highest Pf reported for any biological or artificial membrane.

Prostaglandin-dependent osmotic water permeability of the frog and trout urinary bladder

1998 ◽  
Vol 121 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Yu.V. Natochin ◽  
E.I. Shakhmatova ◽  
Ya.Yu. Komissarchik ◽  
E.S. Snigirevskaya ◽  
N.P. Prutskova ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Water Permeability ◽  
Osmotic Water Permeability ◽  
Osmotic Water

scholarly journals The Effect of Hypertonic Media on Water Permeability of Frog Urinary Bladder

1973 ◽  
Vol 61 (1) ◽  
pp. 110-124 ◽  
Author(s):  
P. Ripoche ◽  
J. Bourguet ◽  
M. Parisi
Keyword(s):  
Epithelial Cells ◽  
Urinary Bladder ◽  
Cyclic Amp ◽  
Antidiuretic Hormone ◽  
Water Permeability ◽  
Prostaglandin E1 ◽  
Physiological State ◽  
Solution Temperature ◽  
Bathing Solution ◽  
Frog Urinary Bladder

The frog urinary bladder undergoes, in some conditions, a marked increase of its water permeability when incubated in hypertonic media. This increase was observed with various nonpermeant solutes. It seems to result from the shrinkage of an osmo-sensitive compartment of the tissue, probably the epithelial cells. Many similarities were found between this effect and the physiological increase in water permeability (hydrosmotic response) elicited by antidiuretic hormone (ADH): both were dependent on the physiological state of the animals, and although the response was slower after hyperosmolar than after hormonal challenge, the patterns of response were similar, and in both cases markedly dependent on bathing solution temperature. Norepinephrine and prostaglandin E1, which in this tissue reduce the hydrosmotic action of ADH, presumably by inhibiting the adenyl cylase also reduced the effect of hyperosmolarity. Conversely this effect was potentiated by incubation in the presence of oxytocin, exogenous cyclic AMP, and theophylline, conditions in which the intracellular concentration of cyclic AMP is increased. These data demonstrate that the response to hyperosmolarity is elicited, at least partly, by mechanisms also involved in the physiological hydrosmotic response to ADH.

Relation of ADH effects to altered membrane fluidity in toad urinary bladder

1981 ◽  
Vol 240 (1) ◽  
pp. F63-F69
Author(s):  
W. A. Kachadorian ◽  
J. Muller ◽  
S. Rudich ◽  
V. A. DiScala
Keyword(s):  
Urinary Bladder ◽  
Membrane Fluidity ◽  
Water Permeability ◽  
Membrane Lipids ◽  
Granular Cell ◽  
Toad Urinary Bladder ◽  
Osmotic Water Permeability ◽  
Intramembranous Particle ◽  
Luminal Membrane ◽  
Osmotic Water

Membrane fluidity, urea permeability, and osmotic water permeability in toad urinary bladder are regularly enhanced by antidiuretic hormone (ADH). In addition, organized intramembranous particle aggregates, which correlate specifically with hormonally stimulated water permeability, are found in granular cell luminal membranes consequent to ADH stimulation. In this investigation ADH-stimulated changes in urea and osmotic water permeability and luminal membrane aggregates at room temperature (24.8 +/- 0.4 degrees C) and in the cold 10.6 +/- 0.2 degrees) were compared with corresponding changes in membrane fluidity, as assessed by n-butyramide permeability. Although a critical level of membrane fluidity is undoubtedly required, the occurrence of aggregates in the luminal membrane is independent of an accompanying hormonally induced change of membrane fluidity. ADH-stimulated osmotic water permeability in toad bladder is also independent of the coincident change in membrane fluidity, and as a process almost certainly involves membrane channels, not a solubility-diffusion process through membrane lipids. For ADH-stimulated transbladder urea movement, channels seem to be involved as well, and the change induced in membrane fluidity by ADH could be an underlying factor in their formation.

scholarly journals Osmotic water permeability of human red cells.

1981 ◽  
Vol 77 (5) ◽  
pp. 549-570 ◽  
Author(s):  
T C Terwilliger ◽  
A K Solomon
Keyword(s):  
Systematic Error ◽  
Water Permeability ◽  
Perturbation Technique ◽  
Mean Value ◽  
Red Cells ◽  
Osmotic Water Permeability ◽  
Osmotic Water ◽  
New Perturbation ◽  
The Relationship ◽  
Human Red Cells

The osmotic water permeability of human red cells has been reexamined with a stopped-flow device and a new perturbation technique. Small osmotic gradients are used to minimize the systematic error caused by nonlinearities in the relationship between cell volume and light scattering. Corrections are then made for residual systematic error. Our results show that the hydraulic conductivity, Lp, is essentially independent of the direction of water flow and of osmolality in the range 184-365 mosM. the mean value of Lp obtained obtained was 1.8 +/- 0.1 (SEM) X 10-11 cm3 dyne -1 s-1.

A method of isolation of apical membranous sheets from frog urinary bladder epithelium by stripping with gelatin

1989 ◽  
Vol 66 (1-2) ◽  
pp. 99-106
Author(s):  
Pierre Favard ◽  
Nina Favard ◽  
Qian Long Zhu ◽  
Jacques Bourguet ◽  
Jean-Pierre Lechaire
Keyword(s):  
Urinary Bladder ◽  
Frog Urinary Bladder ◽  
Bladder Epithelium
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