Novel immunoassay for carcinoembryonic antigen based on protein A-conjugated immunosensor chip by surface plasmon resonance and cyclic voltammetry

2006 ◽  
Vol 28 (5) ◽  
pp. 315-321 ◽  
Author(s):  
Dian-Ping Tang ◽  
Ruo Yuan ◽  
Ya-Qin Chai
Keyword(s):  
Cyclic Voltammetry ◽  
Surface Plasmon Resonance ◽  
Carcinoembryonic Antigen ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Protein A

An immunosensor for the determination of carcinoembryonic antigen by Surface Plasmon Resonance imaging

2020 ◽  
Vol 609 ◽  
pp. 113964
Author(s):  
Beata Szymanska ◽  
Zenon Lukaszewski ◽  
Kinga Hermanowicz-Szamatowicz ◽  
Ewa Gorodkiewicz
Keyword(s):  
Surface Plasmon Resonance ◽  
Carcinoembryonic Antigen ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging

scholarly journals Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

2017 ◽  
Vol 10 (02) ◽  
pp. 1650040 ◽  
Author(s):  
Tao Ma ◽  
Xiaoxia Chen ◽  
Qing Peng ◽  
Pengfei Zhang ◽  
Yonghong He
Keyword(s):  
Aqueous Solution ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Surface Functionalization ◽  
Plasmon Resonance ◽  
Molecular Interaction ◽  
Flow Condition ◽  
Interaction Analysis ◽  
Low Cost ◽  
Protein A

Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR) sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis. These operations are all performed under flow condition in the fluidic system, and have the advantages of easy implementation, less time consuming, and low cost, because the reagents and devices used in the operations are routinely applied in most laboratories. In this study, the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.

Binding of Cu(II)-Poly(N-isopropylacrylamide/vinylimidazole) Copolymer to Histidine-Tagged Protein:  A Surface Plasmon Resonance Study

Langmuir ◽  
2003 ◽  
Vol 19 (3) ◽  
pp. 865-871 ◽  
Author(s):  
A. Kumar ◽  
M. Kamihira ◽  
I. Yu. Galaev ◽  
S. Iijima ◽  
B. Mattiasson
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Protein A
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