Analysis of two-stage continuous operation of Escherichia coli containing bacteriophage λ vector

2000 ◽  
Vol 23 (6) ◽  
pp. 557-563 ◽  
Author(s):  
Seong Hoon Park ◽  
Tai Hyun Park
Keyword(s):  
Escherichia Coli ◽  
Continuous Operation ◽  
Bacteriophage Λ ◽  
Two Stage

Restriction of bacteriophage λ by Escherichia coli K

1973 ◽  
Vol 81 (3) ◽  
pp. 395-407 ◽  
Author(s):  
Noreen E. Murray ◽  
P.L. Batten ◽  
K. Murray
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage Λ

scholarly journals THE INTERACTION OF cos WITH CHI IS SEPARABLE FROM DNA PACKAGING IN recA-recBC-MEDIATED RECOMBINATION OF BACTERIOPHAGE LAMBDA

Genetics ◽  
1983 ◽  
Vol 104 (4) ◽  
pp. 549-570
Author(s):  
Ichizo Kobayashi ◽  
Mary M Stahl ◽  
David Leach ◽  
Franklin W Stahl
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage Lambda ◽  
Dna Packaging ◽  
Bacteriophage Λ ◽  
Entry Site ◽  
Two Component ◽  
General Recombination

ABSTRACT Chi (5′-GCTGGTGG) is a recombinator in RecA- RecBC-mediated recombination in Escherichia coli. In bacteriophage λ vegetative recombination, Chi is fully active only when it is correctly oriented with respect to cos, the site that defines the ends of the packaged chromosome. Here we demonstrate that packaging from cos is not necessary for this cos-Chi interaction. Our evidence suggests that correctly oriented cos is an activator of Chi. cos, as an activator, is (1) dominant over cos  -, (2) active opposite an extensive heterology, (3) able to interact with Chi only when on the same (cis) chromosome, and (4) able to interact with Chi at distances as far as ≥ 20 kb. Thus, cos and Chi form a two-component recombinator system for general recombination. cos may serve as an asymmetric entry site for a recombination enzyme that recognizes Chi in an asymmetric way.

scholarly journals Performance evaluation of combined ultraviolet-ultrasonic technologies in removal of sulfonamide and tetracycline resistant Escherichia coli from domestic effluents

2020 ◽  
Vol 10 (2) ◽  
pp. 276-285 ◽  
Author(s):  
Owassa Dza Rebecca Annisha ◽  
Zifu Li ◽  
Xiaoqin Zhou ◽  
Ngomah Madgil Don Stenay ◽  
Oscar Omondi Donde
Keyword(s):  
Escherichia Coli ◽  
Ultraviolet Radiation ◽  
Continuous Operation ◽  
Pathogenic Microorganisms ◽  
Wastewater Purification ◽  
Antibiotic Resistant ◽  
Radiation Technology ◽  
E Coli ◽  
The Mean ◽  
Domestic Effluents

Abstract Proper treatment of wastewater is key to the achievement of sustainable environmental management. The use of ultraviolet radiation and ultrasound have continued to be considered as some of the best sustainable practices in wastewater purification. However, despite the suitability of the two emerging techniques in sustainably increasing the purification efficiencies of wastewater, their application has not been fully understood, especially in eliminating faecal pathogenic microorganisms. Moreover, their combined potential in the elimination of Escherichia coli resistant genes from wastewater has not been adequately explored. This study was designed to evaluate the potential of individual and combined/integrated ultraviolet radiation and ultrasonic technologies in the removal of antibiotic-resistant E. coli from domestic effluents. There was a statistical difference in the mean log units of sulfonamide resistant E. coli between the different ultraviolet radiation and ultrasonic dosages (P < 0.05), showing that ultraviolet radiation technology was more effective in the removal of both sulfonamide and tetracycline resistant E. coli from the wastewater. However, the integrated ultraviolet radiation-ultrasonic technique was highly efficient and is recommended in the removal of antibiotic resistant E. coli from wastewater. Nonetheless, further studies also need to be performed to further evaluate the disinfection effectiveness on a different bacteria species under continuous operation.

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