Construction of an infectious cDNA clone of Ribgrass mosaic virus Shanghai isolate and its modification to express an epitope of Mycobacterium tuberculosis

2006 ◽  
Vol 151 (9) ◽  
pp. 1853-1861 ◽  
Author(s):  
H. Q. Zhu ◽  
J. P. Chen ◽  
S. Q. Yu
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mosaic Virus ◽  
Cdna Clone ◽  
Infectious Cdna Clone

Construction of an infectious cDNA clone and gene expression vector of Tobacco vein banding mosaic virus (genus Potyvirus)

2012 ◽  
Vol 169 (1) ◽  
pp. 276-281 ◽  
Author(s):  
Rui Gao ◽  
Yan-Ping Tian ◽  
Jie Wang ◽  
Xiao Yin ◽  
Xiang-Dong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mosaic Virus ◽  
Cdna Clone ◽  
Expression Vector ◽  
Infectious Cdna Clone

Brief report: genome sequence and construction of an infectious cDNA clone of Ribgrass mosaic virus from Chinese cabbage in Korea

Virus Genes ◽  
2011 ◽  
Vol 44 (2) ◽  
pp. 345-348 ◽  
Author(s):  
So-Young Ryu ◽  
Jin-Sung Hong ◽  
Sun-Ju Rhee ◽  
Gung Pyo Lee
Keyword(s):  
Mosaic Virus ◽  
Genome Sequence ◽  
Chinese Cabbage ◽  
Cdna Clone ◽  
Infectious Cdna Clone

scholarly journals Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1050
Author(s):  
Liqin Tu ◽  
Shuhua Wu ◽  
Danna Gao ◽  
Yong Liu ◽  
Yuelin Zhu ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Infection Rate ◽  
Cdna Clone ◽  
Infectious Clone ◽  
Infectious Cdna Clone ◽  
Full Length ◽  
Economic Losses ◽  
Chinese Isolate ◽  
Solanum Lycopersicum L ◽  
Rigid Rod

Tomato mottle mosaic virus (ToMMV) is a noteworthy virus which belongs to the Virgaviridae family and causes serious economic losses in tomato. Here, we isolated and cloned the full-length genome of a ToMMV Chinese isolate (ToMMV-LN) from a naturally infected tomato (Solanum lycopersicum L.). Sequence analysis showed that ToMMV-LN contains 6399 nucleotides (nts) and is most closely related to a ToMMV Mexican isolate with a sequence identity of 99.48%. Next, an infectious cDNA clone of ToMMV was constructed by a homologous recombination approach. Both the model host N. benthamiana and the natural hosts tomato and pepper developed severe symptoms upon agroinfiltration with pToMMV, which had a strong infectivity. Electron micrographs indicated that a large number of rigid rod-shaped ToMMV virions were observed from the agroinfiltrated N. benthamiana leaves. Finally, our results also confirmed that tomato plants inoculated with pToMMV led to a high infection rate of 100% in 4–5 weeks post-infiltration (wpi), while pepper plants inoculated with pToMMV led to an infection rate of 40–47% in 4–5 wpi. This is the first report of the development of a full-length infectious cDNA clone of ToMMV. We believe that this infectious clone will enable further studies of ToMMV genes function, pathogenicity and virus–host interaction.

Symptomless infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus

2008 ◽  
Vol 153 (7) ◽  
pp. 1347-1351 ◽  
Author(s):  
Carl Spetz ◽  
Roar Moe ◽  
Dag-Ragnar Blystad
Keyword(s):  
Mosaic Virus ◽  
Cdna Clone ◽  
Infectious Cdna Clone

Construction of an infectious cDNA clone of radish mosaic virus, a crucifer-infecting comovirus

2013 ◽  
Vol 158 (7) ◽  
pp. 1579-1582 ◽  
Author(s):  
Ken Komatsu ◽  
Masayoshi Hashimoto ◽  
Yukari Okano ◽  
Takuya Keima ◽  
Yugo Kitazawa ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Cdna Clone ◽  
Infectious Cdna Clone
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