Identification and characterization of the NTPase activity of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) expressed in bacteria

2007 ◽  
Vol 152 (8) ◽  
pp. 1565-1573 ◽  
Author(s):  
G. Wen ◽  
C. Chen ◽  
X. Luo ◽  
Y. Wang ◽  
C. Zhang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Nonstructural Protein ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Nonstructural Protein 3 ◽  
Identification And Characterization

Characterization of antibody responses against a neutralizing epitope on the glycoprotein E2 of classical swine fever virus

2008 ◽  
Vol 153 (8) ◽  
pp. 1593-1598 ◽  
Author(s):  
Y. Qi ◽  
L. C. Liu ◽  
B. Q. Zhang ◽  
Z. Shen ◽  
J. Wang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Antibody Responses ◽  
Neutralizing Epitope ◽  
Glycoprotein E2

Identification and genetic characterization of classical swine fever virus isolates in Brazil: a new subgenotype

2016 ◽  
Vol 162 (3) ◽  
pp. 817-822 ◽  
Author(s):  
Marta N. F. Silva ◽  
Diogo M. F. Silva ◽  
Adriana S. Leite ◽  
Ana L. V. Gomes ◽  
Antonio C. Freitas ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Genetic Characterization ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Virus Isolates

scholarly journals Characterization of Helper Virus-Independent Cytopathogenic Classical Swine Fever Virus Generated by an In Vivo RNA Recombination System

2005 ◽  
Vol 79 (4) ◽  
pp. 2440-2448 ◽  
Author(s):  
Andreas Gallei ◽  
Till Rümenapf ◽  
Heinz-Jürgen Thiel ◽  
Paul Becher
Keyword(s):  
Classical Swine Fever Virus ◽  
Nonstructural Protein ◽  
Classical Swine Fever ◽  
Helper Virus ◽  
Fever Virus ◽  
Tissue Culture Cell ◽  
Rna Recombination ◽  
Diarrhea Virus ◽  
Recombination System

ABSTRACT Molecular analyses revealed that most cytopathogenic (cp) pestivirus strains evolve from noncytopathogenic (noncp) viruses by nonhomologous RNA recombination. In contrast to bovine viral diarrhea virus (BVDV), cp classical swine fever virus (CSFV) field isolates were rarely detected and always represented helper virus-dependent subgenomes. To investigate RNA recombination in more detail, we recently established an in vivo system allowing the efficient generation of recombinant cp BVDV strains in cell culture after transfecting a synthetic subgenomic and nonreplicatable transcript into cells being infected with noncp BVDV (A. Gallei, A. Pankraz, H.-J. Thiel, and P. Becher, J. Virol. 78:6271-6281, 2004). Using an analogous approach, the first helper virus-independent cp CSFV strain (CP G1) has now been generated by RNA recombination. Accordingly, this study demonstrates the applicability of RNA recombination for designing new viral RNA genomes. The genomic RNA of CP G1 has a calculated size of 18.139 kb, almost 6 kb larger than all previously described CSFV genomes. It contains cellular sequences encoding a polyubiquitin fragment directly upstream of the nonstructural protein NS3 coding gene together with a duplication of viral sequences. CP G1 induces a cytopathic effect on different tissue culture cell lines from pigs and cattle. Subsequent analyses addressed growth kinetics, expression of NS3, and genetic stability of CP G1.

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