scholarly journals Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea

2012 ◽  
Vol 157 (6) ◽  
pp. 1019-1028 ◽  
Author(s):  
Go Atsumi ◽  
Kenji S. Nakahara ◽  
Tomoko Sugikawa Wada ◽  
Sun Hee Choi ◽  
Chikara Masuta ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Rna Silencing ◽  
Yellow Vein ◽  
Clover Yellow Vein Virus ◽  
Viral Suppressors

scholarly journals Unrelated viral suppressors of RNA silencing mediate the control of ARGONAUTE1 level

2013 ◽  
Vol 14 (6) ◽  
pp. 567-575 ◽  
Author(s):  
Éva Várallyay ◽  
Zoltán Havelda
Keyword(s):  
Rna Silencing ◽  
Viral Suppressors

scholarly journals Rapid screening of RNA silencing suppressors by using a recombinant virus derived from beet necrotic yellow vein virus

2009 ◽  
Vol 90 (10) ◽  
pp. 2536-2541 ◽  
Author(s):  
H. Guilley ◽  
D. Bortolamiol ◽  
G. Jonard ◽  
S. Bouzoubaa ◽  
V. Ziegler-Graff
Keyword(s):  
Rna Silencing ◽  
Plant Defence ◽  
Plant Viruses ◽  
Yellow Vein ◽  
Rapid Screening ◽  
Silencing Suppressor ◽  
Defence Mechanisms ◽  
Simple Method ◽  
Yellow Dwarf Virus ◽  
Plant Defence Mechanisms

To counteract plant defence mechanisms, plant viruses have evolved to encode RNA silencing suppressor (RSS) proteins. These proteins can be identified by a range of silencing suppressor assays. Here, we describe a simple method using beet necrotic yellow vein virus (BNYVV) that allows a rapid screening of RSS activity. The viral inoculum consisted of BNYVV RNA1, which encodes proteins involved in viral replication, and two BNYVV-derived replicons: rep3–P30, which expresses the movement protein P30 of tobacco mosaic virus, and rep5–X, which allows the expression of a putative RSS (X). This approach has been validated through the use of several known RSSs. Two potential candidates have been tested and we show that, in our system, the P13 protein of burdock mottle virus displays RSS activity while the P0 protein of cereal yellow dwarf virus-RPV does not.

scholarly journals Rice Stripe Mosaic Virus–Encoded P4 Is a Weak Suppressor of Viral RNA Silencing and Is Required for Disease Symptom Development

2020 ◽  
Vol 33 (3) ◽  
pp. 412-422
Author(s):  
Chao Zhang ◽  
Dong Chen ◽  
Guoyi Yang ◽  
Xiyuan Yu ◽  
Jianguo Wu
Keyword(s):  
Mosaic Virus ◽  
Rna Silencing ◽  
Matrix Protein ◽  
Expression System ◽  
Potato Virus X ◽  
Bimolecular Fluorescence Complementation ◽  
Developmental Defects ◽  
The Matrix ◽  
Silencing Pathways ◽  
Viral Suppressors

Viral suppressors of RNA silencing (VSRs) are a cluster of viral proteins that have evolved to counteract eukaryotic antiviral RNA silencing pathways, thereby contributing to viral pathogenicity. In this study, we revealed that the matrix protein P4 encoded by rice stripe mosaic virus (RSMV), which is an emerging cytoplasmic rhabdovirus, is a weak RNA silencing suppressor. By conducting yeast two-hybrid, bimolecular fluorescence complementation, and subcellular colocalization assays, we proved that P4 interacts with the rice endogenous suppressor of gene silencing 3 (OsSGS3). We also determined that P4 overexpression has no effect on OsSGS3 transcription. However, P4 can promote the degradation of OsSGS3 via ubiquitination and autophagy. Additionally, a potato virus X–based expression system was used to confirm that P4 enhances the development of mosaic symptoms on Nicotiana benthamiana leaves by promoting hydrogen peroxide accumulation but not cell death. To verify whether P4 is a pathogenicity factor in host plants, we generated transgenic P4-overexpressing rice plants that exhibited disease-related developmental defects including decreased plant height and excessive tillering. Our data suggest that RSMV-encoded P4 serves as a weak VSR that inhibits antiviral RNA silencing by targeting OsSGS3.

A STRAIN OF CLOVER YELLOW VEIN VIRUS ISOLATED FROM CALANTHE SP.

1988 ◽  
pp. 61-68 ◽  
Author(s):  
N. Inouye ◽  
T. Maeda ◽  
K. Mitsuhata
Keyword(s):  
Yellow Vein ◽  
Clover Yellow Vein Virus

scholarly journals The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions

2003 ◽  
Vol 84 (10) ◽  
pp. 2861-2869 ◽  
Author(s):  
Ma. Leonora M. Yambao ◽  
Chikara Masuta ◽  
Kenji Nakahara ◽  
Ichiro Uyeda
Keyword(s):  
Amino Acids ◽  
Western Blot ◽  
Yellow Vein ◽  
Transcription Activator ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Clover Yellow Vein Virus ◽  
Two Hybrid ◽  
Far Western Blot

Interactions between the major proteins of Clover yellow vein virus (ClYVV) were investigated using a GAL4 transcription activator-based yeast two-hybrid system (YTHS). Self-interactions manifested by VPg and HCPro and an interaction between NIb and NIaPro were observed in ClYVV. In addition, a strong HCPro–VPg interaction was detected by both YTHS and by in vitro far-Western blot analysis in ClYVV. A potyvirus HCPro–VPg interaction has not been reported previously. Using YTHS, domains in ClYVV for the VPg self-interaction and the HCPro–VPg interaction were mapped. The VPg C-terminal region (38 amino acids) was important for the VPg–VPg interaction and the central 19 amino acids were needed for the HCPro–VPg interaction.

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