scholarly journals Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

2015 ◽  
Vol 160 (7) ◽  
pp. 1775-1779 ◽  
Author(s):  
Masaaki Kitajima ◽  
Andri T. Rachmadi ◽  
Brandon C. Iker ◽  
Eiji Haramoto ◽  
Ian L. Pepper ◽  
...  
Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 798
Author(s):  
Samendra P. Sherchan ◽  
Shalina Shahin ◽  
Jeenal Patel ◽  
Lauren M. Ward ◽  
Sarmila Tandukar ◽  
...  

In this study, we investigated the occurrence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in primary influent (n = 42), secondary effluent (n = 24) and tertiary treated effluent (n = 34) collected from six wastewater treatment plants (WWTPs A–F) in Virginia (WWTP A), Florida (WWTPs B, C, and D), and Georgia (WWTPs E and F) in the United States during April–July 2020. Of the 100 wastewater samples analyzed, eight (19%) untreated wastewater samples collected from the primary influents contained SARS-CoV-2 RNA as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays. SARS-CoV-2 RNA were detected in influent wastewater samples collected from WWTP A (Virginia), WWTPs E and F (Georgia) and WWTP D (Florida). Secondary and tertiary effluent samples were not positive for SARS-CoV-2 RNA indicating the treatment processes in these WWTPs potentially removed SARS-CoV-2 RNA during the secondary and tertiary treatment processes. However, further studies are needed to understand the log removal values (LRVs) and transmission risks of SARS-CoV-2 RNA through analyzing wastewater samples from a wider range of WWTPs.


2010 ◽  
Vol 2010 (15) ◽  
pp. 2236-2240
Author(s):  
Edward T. Furlong ◽  
James L. Gray ◽  
David M. Quanrud ◽  
Sondra S. Teske ◽  
Kathleen Esposito ◽  
...  

2018 ◽  
Vol 77 (7) ◽  
pp. 1810-1818 ◽  
Author(s):  
Courtney M. Gardner ◽  
Carley A. Gwin ◽  
Claudia K. Gunsch

Abstract The use of transgenic crops has become increasingly common in the United States over the last several decades. Increasing evidence suggests that DNA may be protected from enzymatic digestion and acid hydrolysis in the digestive tract, suggesting that crop-derived transgenes may enter into wastewater treatment plants (WWTPs) intact. Given the historical use of antibiotic resistance genes as selection markers in transgenic crop development, it is important to consider the fate of these transgenes. Herein we detected and quantified crop-derived transgenes in WWTPs. All viable US WWTP samples were found to contain multiple gene targets (p35, nos, bla and nptII) at significantly higher levels than control samples. Control wastewater samples obtained from France, where transgenic crops are not cultivated, contained significantly fewer copies of the nptII gene than US activated and digester sludges. No significant differences were measured for the bla antibiotic resistance gene (ARG). In addition, a nested PCR (polymerase chain reaction) assay was developed that targeted the bla ARG located in regions flanked by the p35 promoter and nos terminator. Overall this work suggests that transgenic crops may have provided an environmental source of nptII; however, follow-up studies are needed to ascertain the viability of these genes as they exit WWTPs.


2021 ◽  
Vol 11 (15) ◽  
pp. 6694
Author(s):  
Qing Sun ◽  
Rouzbeh Nazari ◽  
Maryam Karimi ◽  
MD Golam Rabbani Fahad ◽  
Robert W. Peters

Wastewater treatment plants (WWTPs) in the City of New York, United States, are particularly vulnerable to frequent extreme weather events, including storm surges, high-intensity rainfall, and sea level rise, and are also affected by the cascade of these events. The complex structural configuration of WWTPs requires very fine-scale flood risk assessment, which current research has not pursued. We propose a robust technique to quantify the risk of inundations for the fourteen WWPTs through an automated sub-basin creation tool; 889 sub-basins were generated and merged with high-resolution building footprint data to create a comprehensive database for flood inundation analysis. The inundation depths and extents for the WWTPs and flood-prone regions were identified from hydrodynamic modeling of storm surge and sea level rise. The economic damage due to flooding for the WWTPs was also quantified using the HAZUS-MH model. Results indicated that the storm surges from various categories of hurricanes have the dominant impacts on flood depths around WWTPs, followed by high-intensity rainfall. Sea level rise was shown to have a relatively minor impact on flood depths. Results from economic damage analysis showed that the WWTPs are subjected to damage ranging from USD 60,000 to 720,000, depending on the size of the WWTP and the extremity of storm surge. The method of analyzing the inundation status of the research object through the sub-basin enables more accurate data to be obtained when calculating the runoff. It allows for a clearer view of the inundation status of the WWTPs when combined with the actual buildings. Using this database, predicting flood conditions of any extreme event or a cascade of extreme events can be conducted quickly and accurately.


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