Propolis in the control of bacterial bovine mastitis: a tool for the production of organic milk

Bovine mastitis is one of the main causes of economic damage in dairy farms. Therefore, the control and prevention of microorganisms involved in this disease, mainly Escherichia coli, Staphylococcus aureus, and Streptococcus agalactiae, are essential. One of the most important steps for the prevention of the disease is the use of antiseptic products before and after the milking process to avoid bacteria from infecting the udder of the animal. Currently, the most used antiseptic product in dairy farms is iodine-based, and organic dairy farms, which follow several strict regulations, including the use of natural products whenever possible, are often forced to adopt non-natural antiseptic products, such as iodine-based ones, because of the lack of natural alternatives. Propolis, a natural substance produced by honeybees, has been extensively studied for its various properties, one of which is antimicrobial activity. Therefore, a new natural antiseptic product containing 1% propolis in 10% hydroalcoholic solution for the pre-dipping, and 10% glycerol solution added with 0.2% citronella oil for the post-dipping was analyzed for its capacity to reduce bacteria in vivo in order to prevent bovine mastitis, allowing its use on organic dairy farms. A total of 128 samples were analyzed in terms of bacterial growth for Enterobacteriaceae and Staphylococcus spp. using the spreadplate technique. The reduction in the bacterial concentration after the application of the products was compared between two antiseptic solutions, an iodine-based solution as the control and a propolis-based one as the natural alternative. The results obtained show a similar efficiency for both products in terms of total bacterial reduction, indicating considerable antimicrobial activity against bacteria most commonly associated with bovine mastitis. Molecular analysis was carried out for the identification of Streptococcus agalactiae; the PCR results were negative for the presence of S. agalactiae in all samples, indicating that the animals most likely did not have any form of the disease. The efficiency of the natural antiseptic was satisfactory, indicating an important find facilitating organic milk production worldwide, showcasing a natural antiseptic solution with efficient antimicrobial activity.

Triggering Mechanism of Extreme Wind over the Complex Mountain Area in Dali Region on the Yunnan-Guizhou Plateau, China

Wind disasters are responsible for significant physical destruction, injury, loss of life, and economic damage. This study examined the extreme wind triggering mechanism over a typical mountain area with complex terrain, i.e., Dali city in Yunnan Province on the Yunnan-Guizhou Plateau in China. Using the observation data, we first optimized the Weather Research and Forecasting (WRF) model configuration and parametrization schemes for better simulating the wind in this area using a 1-month simulation. Then, the triggering mechanism of extreme wind was investigated by performing a series of sensitive experiments based on a typical extreme wind case. The results indicate that terrain uplift is critical for triggering the local 8–9-scale (the wind velocity between 17.2 and 24.4 m/s) extreme winds over high topography regions. When a large-scale atmospheric circulation is passing, accompanied with regional terrain lifting, the instantaneous wind velocity can reach 9- to 10-scale (the mean wind velocity between 20.8 and 28.4 m/s), causing broken power lines. These results suggest that it is essential to avoid sites where these factors can affect the operation of power transmission lines, or to establish warning systems in the existing systems.

Building a synthesis of economic costs of biological invasions in New Zealand

Biological invasions are a major component of anthropogenic environmental change, incurring substantial economic costs across all sectors of society and ecosystems. However, the economic costs of invasions have been disparately reported, lacking synthesis across taxonomic and sectorial scales. Using the newly compiled InvaCost database, we analyse reported economic damage and management costs incurred by biological invasions in New Zealand — a country renowned for its approaches to invasive species management — from 1968 to 2020. In total, US$69 billion (NZ$97 billion) is currently reported over this ~50 year period, with approximately US$9 billion of this considered highly reliable, observed (c.f. projected) costs. Most (82%) of these observed economic costs are associated with damage, with comparatively little invested in management (18%). Reported costs are increasing over time, with damage averaging US$120 million per year and exceeding management expenditure in all decades. Where specified, most reported costs are from terrestrial plants and animals, with damages most often borne by primary industries such as agriculture and forestry. Management costs are associated more with interventions by authorities and stakeholders. However, some known ecologically (c.f. economically) impactful invasive species are notably absent from estimated damage costs, and management costs are not reported for a number of game animals and agricultural pathogens. Given these gaps for known and potentially damaging invaders, we urge improved cost reporting at the national scale, including improving public accessibility through increased access and digitisation of records, particularly in overlooked socioeconomic sectors and habitats. This also further highlights the importance of investment in management to curtail future damages across all sectors.

Novel Neutralizing Epitope of PEDV S1 Protein Identified by IgM Monoclonal Antibody

Porcine epidemic diarrhea virus (PEDV) causes devastating enteric disease that inflicts huge economic damage on the swine industry worldwide. A safe and highly effective PEDV vaccine that contains only the virus-neutralizing epitopes (not enhancing epitope), as well as a ready-to-use PEDV neutralizing antibody for the passive immunization of PEDV vulnerable piglets (during the first week of life) are needed, particularly for PEDV-endemic farms. In this study, we generated monoclonal antibodies (mAbs) to the recombinant S1 domain of PEDV spike (S) protein and tested their PEDV neutralizing activity by CPE-reduction assay. The mAb secreted by one hybrodoma clone (A3), that also bound to the native S1 counterpart from PEDV-infected cells (tested by combined co-immunoprecipitation and Western blotting), neutralized PEDV infectivity. Epitope of the neutralizing mAb (mAbA3) locates in the S1A subdomain of the spike protein, as identified by phage mimotope search and multiple sequence alignment, and peptide binding-ELISA. The newly identified epitope is shared by PEDV G1 and G2 strains and other alphacoronaviruses. In summary, mAbA3 may be useful as a ready-to-use antibody for passive immunization of PEDV-susceptible piglets, while the novel neutralizing epitope, together with other, previously known protective epitopes, have potential as an immunogenic cocktail for a safe, next-generation PEDV vaccine.

Chromosome-level genome assembly of Bactrocera dorsalis reveals its adaptation and invasion mechanisms

Bactrocera dorsalis is an invasive polyphagous pest causing considerable ecological and economic damage worldwide. We report a high-quality chromosome-level genome assembly and combine various transcriptome data to explore the molecular mechanisms of its rapid adaptation to new environments. The expansions of the DDE transposase superfamily and key gene families related to environmental adaptation and enrichment of the expanded and unique gene families in metabolism and defence response pathways explain its environmental adaptability. The relatively high but not significantly different expression of heat-shock proteins, regardless of the environmental conditions, suggests an intrinsic mechanism underlying its adaptation to high temperatures. The mitogen-activated protein kinase pathway plays a key role in adaptation to new environments. The prevalence of duplicated genes in its genome explains the diversity in the B. dorsalis complex. These findings provide insights into the genetic basis of the invasiveness and diversity of B. dorsalis, explaining its rapid adaptation and expansion.

Analysis of the results of epizootic monitoring of viral diarrhea — a disease of mucous membranes in cattle in the Russian Federation according to the reporting of 4-vet for 2020

Relevance. The reproductive health of dairy cows is one of the top priorities in dairy farming. Viral diarrhea, a disease of mucous membranes in cattle, is one of the causes of impaired reproductive function on farms. The disease is widespread throughout the world and causes significant economic damage, which consists of a decrease in productivity, lack of offspring, impaired reproductive function of the broodstock, costs of treatment and specific prevention. The viral diarrhea virus has an immunosuppressive effect. Diagnosis is complicated by a “blurry” clinical picture due to the association of the virus with other respiratory-intestinal viruses and the addition of a secondary bacterial infection, as well as the presence of a persistent form of infection. The diagnosis requires the analysis of clinical and epizootic data, pathological changes and a combination of several methods of laboratory diagnostics. An important link in the prevention and recovery of herds is the conduct of epizootic monitoring and analysis of its results.Methodology. The assessment of the epizootic situation for viral diarrhea, a disease of mucous membranes in cattle in the Russian Federation for 2020, was carried out based on the results of the analysis of information obtained from annual reports provided by state veterinary laboratories in the FSBI CNMVL in the form of 4-vet (annual).Results. Based on the results of the epizootic monitoring, it can be concluded that the viral diarrhea virus is widely circulating in the livestock farms of the Russian Federation. In 2020, positive results were recorded in all federal districts of the Russian Federation (in 33 subjects), except for the Far Eastern Federal District. The most difficult epizootic situation is in the North Caucasus Federal District and the Central Federal District (38% and 29% of the total number of positive results, respectively).

Irrigation quality and management determine salinization in Israeli olive orchards

Olive (Olea europaea L.) orchard brackish water irrigation with incorrect irrigation management reduces soil fertility and degrades soil health through soil salinization. This study was conducted in the Beit She'an Valley, one of the main agricultural regions in Israel, in an olive orchard in which a combination of soil salinization and poor drainage conditions impedes plant development and causes severe economic damage. By combining various research methods, including soil salinity monitoring, field experiments, remote sensing (frequency domain electromagnetic – FDEM), and unsaturated soil profile saline water movement modeling, the salinization processes were quantified. Irrigation water conductance of 3.13 dS m−1 points to salinization within the tree upper root zone, whereas the modeling results suggest that salinization danger is greater with brackish treated wastewater rather than with lower-salinity brackish irrigation groundwater and that irrigation with potable water can help reduce salt accumulation and recover damaged plots.

Forest canopy resists plant invasions: a case study of Chromolaena odorata in Sal (Shorea robusta) forests of Nepal

Invasive alien species are a major threat to global biodiversity due to the tremendous ecological and economic damage they cause in forestry, agriculture, wetlands, and pastoral resources. Understanding the spatial pattern of invasive alien species and disentangling the biophysical drivers of invasion at the forest stand level is essential for managing forest ecosystems and the wider landscape. However, forest-level and species-specific information on Invasive Alien Plant Species (IAPS) abundance and their spatial extent are largely lacking. In this context, we analysed the cover of one of the world’s worst invasive plants, Chromolaena odorata, in Sal (Shorea robusta) forest in central Nepal. Vegetation was sampled in four community forests using 0.01 ha square quadrats, covering the forest edge to the interior. C. odorata cover, floral richness, tree density, forest canopy cover, shrub cover, tree basal area, and disturbances were measured in each plot. We also explored forest and IAPS management practices in community forests. C. odorata cover was negatively correlated with forest canopy cover, distance to the road, angle of slope, and shrub cover. Tree canopy cover had the largest effect on C. odorata cover. No pattern of C. odorata cover was seen along native species richness gradients. In conclusion, forest canopy cover is the overriding biotic covariate suppressing C. odorata cover in Sal forests.

Abusive Supervision: A Systematic Review and New Research Approaches

Abusive leaders affect employees’ emotions and health and produce counterproductive behaviors that cause economic damage to organizations. The literature has focused predominantly on the antecedents of abusive supervision and its negative impact, providing knowledge on mechanisms that link abusive supervision to consequences for subordinates. There has been limited research on the supervisor perspective, on the group level, and on recovery. This review makes three contributions: first, we examine the theoretical approaches used by previous research studies to understand abusive supervision. Second, we analyze the types of mechanisms that explain how and when an abusive supervision process occurs. Third, we identify and discuss applied methodologies and limitations. Based on the preferred reporting items for systematic reviews and meta-analysis guidelines, and transactional well-being process perspective, we analyzed 171 empirical manuscripts and 239 samples between 2010 and July 2020. We identified a growth in abusive supervision research between 2018 and 2020 and found 101 different theories. Most of these theories view abusive supervision from a social, relational, or affective perspective, but seldom from an emotional perspective. We classified four types of mechanisms: simple relations between abusive supervision and antecedent-consequences (12), moderators (47), mediators (26), and a combination of mediators and moderators (86). We found that research has mostly been performed at the employee level or on dyads; studies that analyze the team level are rarely found. We identified two methodological problems: cross-sectional designs, which do not allow the analysis of its causality, and the increased risk of common method variance that may influence the results obtained via single-source data. In conclusion, the theories used have focused on employee perceptions, which have not enabled the broadening of the abusive supervision concept to include the supervisor’s perspective and a recovery-related perspective. Research on how and when abusive supervision occurs analyzed with complex mechanisms using emotional variables and appropriate daily methodologies has been scarce. We propose a theoretical expansion including emotional theories to uncover emotional consequences of abusive supervision and the recovery concept to provide a deeper insight into abusive supervision process. We contend that longitudinal and diary designs that include teams and supervisor levels are necessary.

Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics

Background Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of daidzein, puerarin. In the US, kudzu is seen as a noxious weed causing ecological and economic damage. However, not all kudzu species make puerarin or are equally invasive. Kudzu remains difficult to identify due to its diverse morphology and inconsistent nomenclature. Results We have generated sequences for the internal transcribed spacer 2 (ITS2) and maturase K (matK) regions of Pueraria montana lobata, P. montana montana, and P. phaseoloides, and identified two accessions previously used for differential analysis of puerarin biosynthesis as P. lobata and P. phaseoloides. Additionally, we have generated root transcriptomes for the puerarin-producing P. m. lobata and the non-puerarin producing P. phaseoloides. Within the transcriptomes, microsatellites were identified to aid in species identification as well as population diversity. Conclusions The barcode sequences generated will aid in fast and efficient identification of the three kudzu species. Additionally, the microsatellites identified from the transcriptomes will aid in genetic analysis. The root transcriptomes also provide a molecular toolkit for comparative gene expression analysis towards elucidation of the biosynthesis of kudzu phytochemicals.