High-throughput sequencing identifies HIV-1-replication- and latency-related miRNAs in CD4+ T cell lines

2017 ◽  
Vol 162 (7) ◽  
pp. 1933-1942 ◽  
Author(s):  
Xiangyun Lu ◽  
Jin Yang ◽  
Haibo Wu ◽  
Zongxing Yang ◽  
Changzhong Jin ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Cd4 T Cell ◽  
T Cell Lines ◽  
Hiv 1

c-Src and Pyk2 Protein Tyrosine Kinases Play Protective Roles in Early HIV-1 Infection of CD4+ T-Cell Lines

2014 ◽  
Vol 66 (2) ◽  
pp. 118-126 ◽  
Author(s):  
Stephen D.S. McCarthy ◽  
Daniel Jung ◽  
Darinka Sakac ◽  
Donald R. Branch
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Tyrosine Kinases ◽  
Protein Tyrosine Kinases ◽  
Cd4 T Cell ◽  
Protein Tyrosine ◽  
T Cell Lines ◽  
Hiv 1

scholarly journals High-Throughput Human Immunodeficiency Virus Type 1 (HIV-1) Full Replication Assay That Includes HIV-1 Vif as an Antiviral Target

2005 ◽  
Vol 49 (9) ◽  
pp. 3833-3841 ◽  
Author(s):  
Joan Cao ◽  
Jason Isaacson ◽  
Amy K. Patick ◽  
Wade S. Blair
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cell ◽  
Cell Lines ◽  
Human Immunodeficiency Virus Type ◽  
High Throughput ◽  
Antiviral Compounds ◽  
Immunodeficiency Virus ◽  
T Cell Lines ◽  
Hiv 1

ABSTRACT Antiviral screens have proved useful for the identification of novel human immunodeficiency virus type 1 (HIV-1) inhibitors. In this study, we describe an HIV-1 full replication (HIV-1 Rep) assay that incorporates all of the targets required for replication in T-cell lines, including the HIV-1 Vif gene. The HIV-1 Rep assay was designed to exhibit optimal sensitivity to late-stage as well as early-stage inhibitors to maximize the likelihood of identification of novel target antiviral compounds in a screen. In addition, the flexibility of the HIV-1 Rep assay allows the rapid evaluation of antiviral compounds against different virus strains in different T-cell lines without significant modification of the assay format. We demonstrate that the HIV-1 Rep assay exhibits characteristics (e.g., a favorable Z′ value) compatible with high-throughput screening in a 384-well format. The utility of the HIV-1 Rep assay was demonstrated in a high-throughput screen of >106 compounds. To our knowledge, this study represents the first example of an HIV-1 antiviral screen that includes Vif as a functional target and was executed on an industrial scale.

Cell-Surface Heparan Sulfate Proteoglycan Mediates HIV-1 Infection of T-Cell Lines

1993 ◽  
Vol 9 (2) ◽  
pp. 167-174 ◽  
Author(s):  
MAHESH PATEL ◽  
MASAKI YANAGISHITA ◽  
GREGORY RODERIQUEZ ◽  
DUMITH CHEQUER BOU-HABIB ◽  
TAMAS ORAVECZ ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Heparan Sulfate ◽  
Cell Lines ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
T Cell Lines ◽  
Hiv 1

scholarly journals Engaging Ly-6A/Sca-1 triggers lipid raft-dependent and -independent responses in CD4+T-cell lines

2017 ◽  
Vol 5 (4) ◽  
pp. 448-460 ◽  
Author(s):  
Melissa A. Lang ◽  
Sultan A. Jenkins ◽  
Phillip Balzano ◽  
Adeyinka Owoyele ◽  
Akshay Patel ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Lipid Raft ◽  
Cd4 T Cell ◽  
T Cell Lines

scholarly journals Differences in the Reactivity of CD4+ T-Cell Lines Generated against Free Versus Nucleosome-Bound SV40 Large T Antigen

2001 ◽  
Vol 53 (4) ◽  
pp. 372-380 ◽  
Author(s):  
G. Bredholt ◽  
O.P. Rekvig ◽  
K. Andreassen ◽  
U. Moens ◽  
T.N. Marion
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
T Antigen ◽  
Cd4 T Cell ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
T Cell Lines

Release of Hydrogen Peroxide from Human T Cell Lines and Normal Lymphocytes Co-Infected with Hiv-1 and Mycoplasma

1995 ◽  
Vol 23 (3) ◽  
pp. 197-212 ◽  
Author(s):  
J. Chochola ◽  
A. D. Strosberg ◽  
M. Stanislawski
Keyword(s):  
Hydrogen Peroxide ◽  
T Cell ◽  
Cell Lines ◽  
Human T Cell ◽  
T Cell Lines ◽  
Hiv 1

Dual expression of CD45RA and CD45RO isoforms on myelin basic protein-specific CD4+ T-cell lines in multiple sclerosis

1993 ◽  
Vol 13 (2) ◽  
pp. 152-161 ◽  
Author(s):  
Yufen Qin ◽  
Stanley van den Noort ◽  
Jeff Kurt ◽  
Sudhir Gupta
Keyword(s):  
Multiple Sclerosis ◽  
T Cell ◽  
Myelin Basic Protein ◽  
Cell Lines ◽  
Basic Protein ◽  
Cd4 T Cell ◽  
T Cell Lines ◽  
Dual Expression
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