Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection

2021 ◽  
Vol 166 (5) ◽  
pp. 1439-1446 ◽  
Author(s):  
Selvin Noé Palacios-Rápalo ◽  
Luis Adrián De Jesús-González ◽  
José Manuel Reyes-Ruiz ◽  
Juan Fidel Osuna-Ramos ◽  
Carlos Noe Farfan-Morales ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Nuclear Localization ◽  
Structural Protein ◽  
Dengue Virus Infection

scholarly journals Increased Plasma Heparanase Activity and Endothelial Glycocalyx Degradation in Dengue Patients Is Associated With Plasma Leakage

2021 ◽  
Vol 12 ◽  
Author(s):  
Baranca Buijsers ◽  
Fadel Muhammad Garishah ◽  
Silvita Fitri Riswari ◽  
Rosalie M. van Ast ◽  
Setyo Gundi Pramudo ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Heparan Sulfate ◽  
Ex Vivo ◽  
Endothelial Glycocalyx ◽  
Severe Dengue ◽  
Structural Protein ◽  
Dengue Virus Infection ◽  
Activated Platelets ◽  
Plasma Leakage

BackgroundEndothelial hyper-permeability with plasma leakage and thrombocytopenia are predominant features of severe dengue virus infection. It is well established that heparanase, the endothelial glycocalyx degrading enzyme, plays a major role in various diseases with vascular leakage. It is yet to be elucidated whether heparanase activity plays a major role in dengue-associated plasma leakage. Moreover, the major source of heparanase secretion and activation in dengue remains elusive. Since a relatively high amount of heparanase is stored in platelets, we postulate that heparanase released by activated platelets contributes to the increased plasma heparanase activity during dengue virus infection.MethodsHeparanase activity (plasma and urine), and heparan sulfate and syndecan-1 (plasma levels) were measured in dengue patients with thrombocytopenia in acute phase (n=30), during course of disease (n=10) and in convalescent phase (n=25). Associations with clinical parameters and plasma leakage markers were explored. Platelets from healthy donors were stimulated with dengue non-structural protein-1, DENV2 virus and thrombin to evaluate heparanase release and activity ex vivo.ResultsHeparanase activity was elevated in acute dengue and normalized during convalescence. Similarly, glycocalyx components, such as heparan sulfate and syndecan-1, were increased in acute dengue and restored during convalescence. Increased heparanase activity correlated with the endothelial dysfunction markers heparan sulfate and syndecan-1, as well as clinical markers of plasma leakage such as ascites, hematocrit concentration and gall-bladder wall thickening. Notably, platelet number inversely correlated with heparanase activity. Ex vivo incubation of platelets with thrombin and live DENV2 virus, but not dengue virus-2-derived non-structural protein 1 induced heparanase release from platelets.ConclusionTaken together, our findings suggest that the increase of heparanase activity in dengue patients is associated with endothelial glycocalyx degradation and plasma leakage. Furthermore, thrombin or DENV2 activated platelets may be considered as a potential source of heparanase.

Mouse Model of Dengue Virus Infection with Serotypes 1 and 2 Clinical Isolates

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (23) ◽  
Author(s):  
Satoru Watanabe ◽  
Kitti Wing Chan ◽  
Subhash Vasudevan
Keyword(s):  
Virus Infection ◽  
Mouse Model ◽  
Dengue Virus ◽  
Clinical Isolates ◽  
Dengue Virus Infection

scholarly journals An intelligent healthcare system for predicting and preventing dengue virus infection

Computing ◽  
2021 ◽  
Author(s):  
Sandeep Kumar Sood ◽  
Vaishali Sood ◽  
Isha Mahajan ◽  
Sahil
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Healthcare System ◽  
Dengue Virus Infection ◽  
Intelligent Healthcare
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