Genome characterization of zucchini yellow mosaic virus infecting cucurbits reveals the presence of a new genotype in Trinidad and Tobago in the Caribbean region

2021 ◽  
Author(s):  
Chinnaraja Chinnadurai ◽  
Mounika Kollam ◽  
Adesh Ramsubhag ◽  
Jayaraj Jayaraman
Keyword(s):  
Trinidad And Tobago ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Caribbean Region ◽  
Genome Characterization ◽  
New Genotype ◽  
The Caribbean

scholarly journals Genome characterization of Zucchini yellow mosaic virus infecting cucurbits reveals the presence of a new genotype in Trinidad & Tobago in the Caribbean region

2021 ◽  
Author(s):  
Chinnadurai Chinnaraja ◽  
Mounika Kollam ◽  
Adesh Ramsubhag ◽  
Jayaraman Jayaraj
Keyword(s):  
Trinidad And Tobago ◽  
Mosaic Virus ◽  
Complete Genome ◽  
Phylogenetic Analyses ◽  
Aphis Gossypii ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Caribbean Region ◽  
Gene Sequences ◽  
New Genotype

Abstract Zucchini yellow mosaic virus is a potyvirus, which is becoming a serious pathogen of pumpkin and other cucurbits in Trinidad and Tobago and the entire Caribbean region. In this study, four Zucchini yellow mosaic virus (ZYMV) isolates infecting pumpkin in Trinidad and Tobago were characterized by complete genome sequencing for the first time. Phylogenetic analyses of the isolates showed variability of 5.9–6.0 % nt and 7.7–7.9 % aa sequences with the most closely related isolates NAT and AG (Israel) and SE04T (Slovakia). Based on the variations in complete genome as well as gene sequences, a new genotype designated ZYMV-Trini is proposed for these isolates. Among the gene sequences of ZYMV-Trini isolates, maximum variations were noticed in HC-Pro gene with 20.8 % aa sequence divergence from their closest relatives whereas the least variations were with the NIb, P3 and CP genes (1.8 to 2.2 % aa divergence). This study also proved that transmission of ZYMV can also occur through seeds (2 %), but this was less common than transmission via the aphid species Aphis gossypii. The progression of ZYMV in pumpkin seedlings was quantified by RT-qPCR which showed a rapid surge in viral load after 37 days. From the recombination analyses, it could be concluded that isolates SE04T from Slovakia, NAT from Israel and AG from Israel have major contributions in the genome architecture of ZYMV-Trini isolates.

Characterization of a variant isolate of Zucchini yellow mosaic virus infecting green kabocha (Cucurbita maxima L.) in Bogor, Indonesia

2021 ◽  
pp. 1-8
Author(s):  
Tri Asmira Damayanti ◽  
Titah Nurjannah ◽  
Listihani Listihani ◽  
Sri Hendrastuti Hidayat ◽  
Suryo Wiyono
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Cucurbita Maxima

scholarly journals Two Newly Described Begomoviruses of Macroptilium lathyroides and Common Bean

2003 ◽  
Vol 93 (7) ◽  
pp. 774-783 ◽  
Author(s):  
A. M. Idris ◽  
E. Hiebert ◽  
J. Bird ◽  
J. K. Brown
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Dna Sequences ◽  
Western Hemisphere ◽  
Yellow Mosaic Virus ◽  
Nucleotide Identity ◽  
Caribbean Region ◽  
Golden Yellow ◽  
The Caribbean ◽  
Macroptilium Lathyroides

Macroptilium lathyroides, a perennial weed in the Caribbean region and Central America, is a host of Macroptilium yellow mosaic Florida virus (MaYMFV) and Macroptilium mosaic Puerto Rico virus (MaMPRV). The genomes of MaYMFV and MaMPRV were cloned from M. lathyroides and/or field-infected bean and the DNA sequences were determined. Cloned A and B components for both viruses were infectious when inoculated to M. lathyroides and common bean. Comparison of the DNA sequences for cloned A and B components with well-studied begomovirus indicated that MaMPRV (bean and M. lathyroides) and MaYMFV (M. lathyroides) are unique, previously undescribed begomo-viruses from the Western Hemisphere. Phylogenetic analysis of viral A components indicated that the closest relative of MaYMFV are members of the Bean golden yellow mosaic virus (BGYMV) group, at 76 to 78% nucleotide identity, whereas the closest relative for the A component of MaMPRV was Rhynchosia golden mosaic virus at 78% nucleotide identity. In contrast, BGYMV is the closest relative for the B component of both MaYMFV and MaMPRV, with which they share ≈68.0 and ≈72% identity, respectively. The incongruent taxonomic placement for the bipartite components for MaMPRV indicates that they did not evolve entirely along a common path. MaYMFV and MaMPRV caused distinctive symptoms in bean and M. lathyroides and were transmissible by the whitefly vector and by grafting; however, only MaYMFV was mechanically transmissible. The experimental host range for the two viruses was similar and included species within the families Fabaceae and Malvaceae, but only MaYMFV infected Malva parviflora and soybean. These results collectively indicate that MaMPRV and MaYMFV are new, previously undescribed species of the BGYMV group, a clade previously known to contain only strains and isolates of BGYMV from the Caribbean region that infect Phaseolus spp. Both MaYMFV and MaMPRV may pose an economic threat to bean production in the region.

scholarly journals Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia

2012 ◽  
Vol 49 (2) ◽  
pp. 151-159
Author(s):  
Vucurovic Ana ◽  
Bulajic Aleksandra ◽  
Milojevic Katarina ◽  
Stankovic Ivana ◽  
Ristic Danijela ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus

Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

2012 ◽  
Vol 133 (4) ◽  
pp. 935-947 ◽  
Author(s):  
Ana Vučurović ◽  
Aleksandra Bulajić ◽  
Ivana Stanković ◽  
Danijela Ristić ◽  
Janoš Berenji ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Partial Characterization ◽  
Yellow Mosaic Virus ◽  
Virus Isolates

scholarly journals Characterization of different isolates of Zucchini yellow mosaic virus from cucurbits in Saudi Arabia

2014 ◽  
Vol 8 (19) ◽  
pp. 1987-1994 ◽  
Author(s):  
A. Al-Saleh Mohammed ◽  
A. Amer Mahmoud ◽  
M. AL-Shahwan Ibrahim ◽  
A. Abdalla Omer ◽  
A. Zakri Mohammed
Keyword(s):  
Saudi Arabia ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus

scholarly journals Frequency and molecular characterization of watermelon mosaic virus from Serbia

2010 ◽  
Vol 25 (3) ◽  
pp. 213-230
Author(s):  
Ana Vucurovic ◽  
Aleksandra Bulajic ◽  
Ivana Stankovic ◽  
Danijela Ristic ◽  
Janos Berenji ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Growing Season ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Mixed Infections ◽  
Mediterranean Regions

Watermelon mosaic virus (WMV) is widespread in cucurbit crops, most commonly occuring in temperate and Mediterranean regions. In Serbia WMV has been detected in single and mixed infections with Zucchini yellow mosaic virus and Cucumber mosaic virus in field-grown pumpkin and squash crops. Among pumpkin-affecting viruses WMV is the most frequent one, both by the number of localities and its incidence at each location. During the growing season of 2009, samples from 583 plants of Cucurbita pepo cvs. Olinka, Belgrade zucchini and Tosca (Zucchini group), as well as from C. maxima and C. moschata showing symptoms of virus infection were collected from 12 commercial fields at eight localities and analyzed by DAS-ELI

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