scholarly journals Complete genome sequence and construction of an infectious full-length cDNA clone of a cucumber vein yellowing virus (CVYV) isolate from Portugal

2021 ◽  
Author(s):  
K. Cordes ◽  
E. Maiss ◽  
S. Winter ◽  
H. Rose
Keyword(s):  
Cdna Clone ◽  
Complete Genome ◽  
Virus Transmission ◽  
Complete Sequence ◽  
Full Length ◽  
Wild Type Virus ◽  
Gibson Assembly ◽  
Full Length Cdna ◽  
The Family ◽  
Yellowing Virus

AbstractCucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus in the family Potyviridae. In the National Center for Biotechnology Information (NCBI) database, three complete genome sequences of CVYV isolates from Spain (NC_006941), Israel (KT276369), and Jordan (JF460793) are available. In this study, we report the complete sequence of an isolate of CVYV from Portugal (DSMZ PV-0776) along with the construction of an infectious full-length cDNA clone via Gibson assembly. The sequence of CVYV Portugal shows the closest relationship to a CVYV isolate from Spain (genome, 99.7% identity; polyprotein, 99.7% identity). The CVYV full-length cDNA clone was introduced by electroporation into Rhizobium radiobacter and infiltrated into the cotyledons of Cucumis sativus plantlets, resulting in symptoms resembling those of the wild-type virus. Transmission of the infectious CVYV full-length clone by the whitefly Bemisia tabaci was confirmed. This first report confirming the infectivity of a CVYV cDNA clone provides the opportunity to study gene functions in a consistent genomic background.

scholarly journals Biological properties of Beet mild yellowing virus derived from a full-length cDNA clone

2006 ◽  
Vol 87 (2) ◽  
pp. 445-449 ◽  
Author(s):  
Dirk Stephan ◽  
Edgar Maiss
Keyword(s):  
Host Range ◽  
Cdna Clone ◽  
Genomic Sequence ◽  
Aphid Transmission ◽  
Biological Properties ◽  
Full Length ◽  
Nicotiana Glutinosa ◽  
Full Length Cdna ◽  
Beet Mild Yellowing Virus ◽  
Yellowing Virus

A German isolate of Beet mild yellowing virus (BMYV-IPP) was used for RT-PCR-based construction of the first infectious full-length cDNA clone of the virus (BMYVfl). The complete genomic sequence was determined and displayed high similarity to the French isolate BMYV-2ITB. The host range of BMYVfl was examined by agroinoculation and aphid transmission. Both methods lead to systemic infections in Beta vulgaris, Nicotiana benthamiana, N. clevelandii, N. hesperis, Capsella bursa-pastoris and Lamium purpureum. Immunological investigation by tissue-print immunoassay (TPIA) of agroinoculated plant tissues revealed only local infections restricted to the agroinoculated mesophyll tissues in some plant species. In Nicotiana glutinosa and N. edwardsonii, BMYV was not found in either the agroinoculated tissue or distant tissues by TPIA. So far, BMYVfl agroinoculation did not extend or confine the BMYV host range known from aphid transmission experiments but it did describe new local hosts for BMYV.

Construction of an infectious full-length cDNA clone of potato aucuba mosaic virus

2021 ◽  
Vol 166 (5) ◽  
pp. 1427-1431
Author(s):  
Buyang Chen ◽  
Qi Lin ◽  
Yueyan Yin ◽  
Liangliang Jiang ◽  
Fang Wang ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Cdna Clone ◽  
Full Length ◽  
Full Length Cdna ◽  
Aucuba Mosaic

Construction of an infectious full-length cDNA clone of Chrysanthemum virus B

2008 ◽  
Vol 74 (6) ◽  
pp. 434-437 ◽  
Author(s):  
Atsushi Ohkawa ◽  
Noriko Ishikawa-Suehiro ◽  
Seiichi Okuda ◽  
Tomohide Natsuaki
Keyword(s):  
Cdna Clone ◽  
Full Length ◽  
Full Length Cdna

scholarly journals Nucleotide sequence of a full-length cDNA clone encoding preproparathyroid hormone from pig and rat

1987 ◽  
Vol 15 (16) ◽  
pp. 6740-6740 ◽  
Author(s):  
Hans-Jürgen Schmelzer ◽  
Gerhard Gross ◽  
Georg Widera ◽  
Hubert Mayer
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Full Length ◽  
Full Length Cdna

scholarly journals Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins.

1994 ◽  
Vol 5 (12) ◽  
pp. 1301-1310 ◽  
Author(s):  
S W Clark ◽  
O Staub ◽  
I B Clark ◽  
E L Holzbaur ◽  
B M Paschal ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cdna Clone ◽  
Expressed Sequence Tag ◽  
Full Length ◽  
Northern Analysis ◽  
Related Protein ◽  
Two Dimensional Gel Electrophoresis ◽  
Full Length Cdna ◽  
Dynactin Complex ◽  
Expressed Sequence

An examination of human-expressed sequence tags indicated the existence of an isoform of centractin, an actin-related protein localized to microtubule-associated structures. Using one of these tags, we isolated and determined the nucleotide sequence of a full-length cDNA clone. The protein encoded represents the first example of multiple isoforms of an actin-related protein in a single organism. Northern analysis using centractin-specific probes revealed three species of mRNA in HeLa cells that could encode centractin isoforms. One mRNA encodes the previously-identified centractin (now referred to as alpha-centractin). The full-length cDNA clone isolated using the expressed sequence tag encodes a new member of the centractin family, beta-centractin. A probe specific for alpha-centractin hybridized to the third species of mRNA observed (referred to as gamma-centractin). Comparisons of Northern blots of human tissues indicated that alpha-centractin and beta-centractin mRNAs are equally distributed in all populations of mRNA examined, whereas the expression of gamma-centractin appears to be tissue specific. The amino acid sequence of beta-centractin, deduced from the cDNA, indicates a 91% identity with alpha-centractin, increasing to 96% similarity when conservative amino acid changes are taken into account. As antibodies previously raised against alpha-centractin reacted only poorly with beta-centractin, new antibodies were produced and combined with two-dimensional gel electrophoresis to discriminate the two isoforms. Using this system, the subcellular distribution of the alpha- and beta-isoforms were determined. Both isoforms were found predominantly in the cytosolic fraction as a part of a previously identified 20S complex (referred to as the dynactin complex) with no evidence for a free pool of either isoform. The isoforms were found in a constant ratio of approximately 15:1 (alpha:beta) in the dynactin complex.

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