Protective effects of appropriate Zn2+ levels against UVB radiation-induced damage in human lens epithelial cells in vitro

2015 ◽  
Vol 21 (2) ◽  
pp. 213-226 ◽  
Author(s):  
Yuxiang Du ◽  
Dadong Guo ◽  
Qiuxin Wu ◽  
Jing Shi ◽  
Dongmei Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Protective Effects ◽  
Uvb Radiation ◽  
Human Lens Epithelial Cells ◽  
Radiation Induced

scholarly journals P 229 Growth of human lens epithelial cells on the anterior lens capsule in vitro

1995 ◽  
Vol 35 ◽  
pp. S199
Author(s):  
J.H. Meyer ◽  
J. Schmidt ◽  
F. Eppinger ◽  
B. Flügel ◽  
K.U. Löffler ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lens Capsule ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Anterior Lens Capsule

scholarly journals On the Anticataractogenic Effects of L-Carnosine: Is It Best Described as an Antioxidant, Metal-Chelating Agent or Glycation Inhibitor?

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Hamdy Abdelkader ◽  
Michael Longman ◽  
Raid G. Alany ◽  
Barbara Pierscionek
Keyword(s):  
Epithelial Cells ◽  
Antioxidant Activities ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Metal Chelating ◽  
Human Lens Epithelial Cells

Purpose.L-Carnosine is a naturally occurring dipeptide which recently gained popularity as an anticataractogenic agent due to its purported antioxidant activities. There is a paucity of research and conclusive evidence to support such claims. This work offers compelling data that help clarify the mechanism(s) behind the anticataract properties of L-carnosine.Methods.Direct in vitro antioxidant free radical scavenging properties were assayed using three different antioxidant (TEAC, CUPRAC, and DPPH) assays. Indirect in vitro and ex vivo antioxidant assays were studied by measuring glutathione bleaching capacity and total sulfhydryl (SH) capacity of bovine lens homogenates as well as hydrogen-peroxide-stress assay using human lens epithelial cells. Whole porcine lenses were incubated in high galactose media to study the anticataract effects of L-carnosine. MTT cytotoxicity assays were conducted on human lens epithelial cells.Results.The results showed that L-carnosine is a highly potent antiglycating agent but with weak metal chelating and antioxidant properties. There were no significant decreases in lens epithelial cell viability compared to negative controls. Whole porcine lenses incubated in high galactose media and treated with 20 mM L-carnosine showed a dramatic inhibition of advanced glycation end product formation as evidenced by NBT and boronate affinity chromatography assays.Conclusion.L-Carnosine offers prospects for investigating new methods of treatment for diabetic cataract and any diseases that are caused by glycation.

TGF-ß-induced apoptosis and reduction of Bcl-2 in human lens epithelial cells in vitro

2002 ◽  
Vol 25 (3) ◽  
pp. 147-153 ◽  
Author(s):  
Joon H. Lee ◽  
Xiu Hua Wan ◽  
Jeongmin Song ◽  
Jimmy Jaeyoung Kang ◽  
Woo-Sook Chung ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Induced Apoptosis

Specific features of apoptosis in human lens epithelial cells induced by mitomycin C in vitro

2001 ◽  
Vol 239 (8) ◽  
pp. 613-618 ◽  
Author(s):  
J. Jordan ◽  
N. Kociok ◽  
S. Grisanti ◽  
P. Jacobi ◽  
J. Esser ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mitomycin C ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells
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