Enhanced bone formation of calvarial bone defects by low-intensity pulsed ultrasound and recombinant human bone morphogenetic protein-9: a preliminary experimental study in rats

The best way in which to prepare scaffolds with good biological properties is an urgent problem in the field of tissue engineering. In this paper we discuss the preparation of nano-hydroxyapatite scaffold of recombinant human bone morphogenetic protein-2 (rhBMP-2) and its application in bone defect repair. rhBMP-2 reagent was dissolved in 1 mol/L sodium dihydrogen phosphate solution, and the rhBMP-2 solution was added to the nano-hydroxyapatite artificial bone with a 100 μL glass micro dropper at the rate of 10 drops/min to obtain Nano-HA/rhBMP-2 composite artificial bone. In in vivo experiments, rabbits were fixed on an operating table, a 2 cm longitudinal incision was made in the middle part of the radial forearm, and the radius was cut with a wire saw and periosteum, 2.5 cm away from the distal radius. After washing the wound with normal saline, Adv-hBMP-2/MC3T3-E1 nano-HA composite artificial bone, MC3T3-E1 nan-HA composite artificial bone, or Nano-HA artificial bone were implanted in different groups. The artificial bone scaffold prepared in this study has a stronger ability to repair bone defects than the alternatives, and is a promising prospect for the clinical treatment of bone defects.

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A Histological Study on Experimental Tooth Movement into Bone Induced by Recombinant Human Bone Morphogenetic Protein-2 in Beagle Dogs

The Cleft Palate-Craniofacial Journal ◽

10.1597/1545-1569_2002_039_0439_ahsoet_2.0.co_2 ◽

2002 ◽

Vol 39 (4) ◽

pp. 439-448 ◽

Cited By ~ 3

Author(s):

Tatsuo Kawamoto ◽

Nobuyoshi Motohashi ◽

Atsushi Kitamura ◽

Yoshiyuki Baba ◽

Koichiro Takahashi ◽

...

Keyword(s):

Bone Morphogenetic Protein ◽

Tooth Movement ◽

Bone Defects ◽

Bone Morphogenetic Protein 2 ◽

Control Group ◽

Beagle Dogs ◽

Morphogenetic Protein ◽

Human Bone Morphogenetic Protein ◽

Recombinant Human Bone ◽

Experimental Tooth Movement

Objective The purpose of this preliminary study was to examine experimental tooth movement into newly generated bone induced by recombinant human bone morphogenetic protein-2 (rhBMP-2). Method After extraction of the maxillary first premolars, bone defects were surgically created in eight adult beagle dogs using a 5-mm-diameter trepan bar. According to which material was grafted into the bone defects, animals were divided into the following four groups: (1) the rhBMP-2 group in which rhBMP-2 with a poly [D,L-(lactide-co-glycolide)]/gelatin sponge complex was implanted; (2) the spongiosa group in which spongiosa from the tibia was grafted; (3) the nongrafted group in which no material was embedded; and (4) the control group in which only tooth extraction was performed. The osteoinductive activity of rhBMP-2 and tooth movement into the newly generated bone were examined by histological and morphometric comparisons of each group. Results Considerable new bone formation was observed at the grafted site both in the rhBMP-2 and in the spongiosa groups. The area of generated bone in the rhBMP-2 group was significantly greater than that in the spongiosa group. Newly generated bone, in both the rhBMP-2 and spongisosa groups, showed a similar histological response to orthodontic force as in normal alveolar bone in the control group. However, root resorption occurred on the pressure side in the rhBMP-2 group. Conclusion These results indicated that rhBMP-2 might constitute an alternative material to autogeneous bone grafting for alveolar cleft defects. Further studies regarding tooth movement into generated bone induced by rhBMP-2 are suggested.

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Electroactive barium titanate coated titanium scaffold improves osteogenesis and osseointegration with low-intensity pulsed ultrasound for large segmental bone defects

Bioactive Materials ◽

10.1016/j.bioactmat.2020.07.001 ◽

2020 ◽

Vol 5 (4) ◽

pp. 1087-1101 ◽

Cited By ~ 3

Author(s):

Bo Fan ◽

Zheng Guo ◽

Xiaokang Li ◽

Songkai Li ◽

Peng Gao ◽

...

Keyword(s):

Barium Titanate ◽

Bone Defects ◽

Pulsed Ultrasound ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity ◽

Segmental Bone ◽

Segmental Bone Defects

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Histological and Radiological Evaluation of Low-Intensity Pulsed Ultrasound Versus Whole Body Vibration on Healing of Mandibular Bone Defects in Rats

Medicina ◽

10.3390/medicina56090457 ◽

2020 ◽

Vol 56 (9) ◽

pp. 457

Author(s):

Milad Etemadi Sh ◽

Nan-Chen Hsieh ◽

Seyed Shahin Movahed Mohammadi ◽

Shahrooz Momeni ◽

Seyed Mohammad Razavi ◽

...

Keyword(s):

Bone Density ◽

Whole Body Vibration ◽

Bone Defects ◽

Whole Body ◽

Control Group ◽

Pulsed Ultrasound ◽

Mandibular Bone ◽

Body Vibration ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity

Background and Objectives: Mechanical stimulation can improve the structural properties of the fracture site and induce the differentiation of different cell types for bone regeneration. This study aimed to compare the effect of low-intensity pulsed ultrasound stimulation (LIPUS) versus whole body vibration (WBV) on healing of mandibular bone defects. Materials and Methods: A mandibular defect was created in 66 rats. The rats were randomly divided into two groups of rats. Each group was subdivided randomly by three groups (n = 11) as follows: (I) control group, (II) treatment with LIPUS, and (III) treatment with WBV. The radiographic changes in bone density, the ratio of lamellar bone to the entire bone volume, the ratio of the newly formed bone to the connective tissue and inflammation grade were evaluated after 1 and 2 months. Results: LIPUS significantly increased the radiographic bone density change compared to the control group at the first and second month postoperatively (p < 0.01). WBV only significantly increased the bone density compared to the control group at the second month after the surgery (p < 0.01). Conclusions: Application of LIPUS and WBV may enhance the regeneration of mandibular bone defects in rats. Although LIPUS and WBV are effective in mandibular bone healing, the effects of LIPUS are faster and greater than WBV.

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