Understanding thermal and organic solvent stability of thermoalkalophilic lipases: insights from computational predictions and experiments

2020 ◽  
Vol 26 (6) ◽  
Author(s):  
Mohamed Shehata ◽  
Emel Timucin ◽  
Alessandro Venturini ◽  
Osman Uğur Sezerman
Keyword(s):  
Organic Solvent ◽  
Solvent Stability ◽  
Computational Predictions

scholarly journals Understanding Thermal and Organic Solvent Stability of Thermoalkalophilic Lipases: Insights from Computational Predictions and Experiments

2019 ◽  
Author(s):  
Mohamed Shehata ◽  
Emel Timucin ◽  
Alessandro Venturini ◽  
Osman Ugur Sezerman
Keyword(s):  
Organic Solvent ◽  
In Silico ◽  
High Stability ◽  
Extreme Conditions ◽  
Solvent Stability ◽  
The Family ◽  
Structural Impact ◽  
Different Temperatures ◽  
Experimental Findings ◽  
Computational Predictions

The study enclosed herein reports computational and experimental findings on the active and inactive conformations of the lipase from the family I.5. Given their high stability under extreme conditions, this lipase family, also called thermoalkalophilic liapses, are of high potential for industrial reactions. To this end, this study investigated the structural impact of 5 different organic solvents that are commonly used in industrial reactions, on the active and inactive conformations <i>in silico</i>. These findings were validated by the experiments which recruited the recombinant enzyme and analyzed organic solvent stability at different temperatures. <br>

scholarly journals Understanding Thermal and Organic Solvent Stability of Thermoalkalophilic Lipases: Insights from Computational Predictions and Experiments

2019 ◽  
Author(s):  
Mohamed Shehata ◽  
Emel Timucin ◽  
Alessandro Venturini ◽  
Osman Ugur Sezerman
Keyword(s):  
Organic Solvent ◽  
In Silico ◽  
High Stability ◽  
Extreme Conditions ◽  
Solvent Stability ◽  
The Family ◽  
Structural Impact ◽  
Different Temperatures ◽  
Experimental Findings ◽  
Computational Predictions

The study enclosed herein reports computational and experimental findings on the active and inactive conformations of the lipase from the family I.5. Given their high stability under extreme conditions, this lipase family, also called thermoalkalophilic liapses, are of high potential for industrial reactions. To this end, this study investigated the structural impact of 5 different organic solvents that are commonly used in industrial reactions, on the active and inactive conformations <i>in silico</i>. These findings were validated by the experiments which recruited the recombinant enzyme and analyzed organic solvent stability at different temperatures. <br>

Improvement of the organic solvent stability of a commercial lipase by chemical modification with dextran

2019 ◽  
Vol 142 ◽  
pp. 1-6 ◽  
Author(s):  
Shota Kajiwara ◽  
Kyohei Komatsu ◽  
Ryosuke Yamada ◽  
Takuya Matsumoto ◽  
Masahiro Yasuda ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Chemical Modification ◽  
Solvent Stability ◽  
Commercial Lipase

Unraveling the Rationale Behind Organic Solvent Stability of Lipases

2012 ◽  
Vol 167 (3) ◽  
pp. 439-461 ◽  
Author(s):  
Debamitra Chakravorty ◽  
Saravanan Parameswaran ◽  
Vikash Kumar Dubey ◽  
Sanjukta Patra
Keyword(s):  
Organic Solvent ◽  
Solvent Stability

Organic-solvent stability of elastase strain K overexpressed in anEscherichia–Pseudomonasexpression system

2010 ◽  
Vol 57 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Chee Fah Wong ◽  
Abu Bakar Salleh ◽  
Mahiran Basri ◽  
Raja Noor Zaliha Raja Abd. Rahman
Keyword(s):  
Organic Solvent ◽  
Solvent Stability

Modification of lipase from Candida cylindracea with dextran using the borane-pyridine complex to improve organic solvent stability

2019 ◽  
Vol 296 ◽  
pp. 1-6 ◽  
Author(s):  
Shota Kajiwara ◽  
Kyohei Komatsu ◽  
Ryosuke Yamada ◽  
Takuya Matsumoto ◽  
Masahiro Yasuda ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Candida Cylindracea ◽  
Solvent Stability ◽  
Pyridine Complex

Effect of exchange of amino acid residues of the surface region of the PST-01 protease on its organic solvent-stability

2007 ◽  
Vol 358 (4) ◽  
pp. 1028-1033 ◽  
Author(s):  
Hiroyasu Ogino ◽  
Takeshi Uchiho ◽  
Noriyuki Doukyu ◽  
Masahiro Yasuda ◽  
Kosaku Ishimi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Organic Solvent ◽  
Surface Region ◽  
Amino Acid Residues ◽  
Solvent Stability
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