Polymorphisms of the Calpain10 and TCF7L2 genes were identified as possible
type 2 diabetes susceptibility genetic markers. We conducted a case-control
study to evaluate the relation between SNP43 of calpain-10 and rs12255372 and
rs7903146 in the TCF7L2 with type2 diabetes in western-north of Iran. The
role of these variants in Iranian population was less clear. A total of 202
patients and healthy controls were enrolled to analysis the frequency
distribution of Calpain10 and TCF7L2 polymorphisms (SNP43, rs12255372 and
rs7903146) using polymerase chain reaction-restriction fragment length
polymorphism (PCR - RFLP) method. The frequency of allele A in controls was
significantly greater than that of diabetic patients (P=0.031), whereas the
difference between distribution of SNP43 genotypes (A/A, A/G, G/G) were non-
significant in case and control groups. Non significant association was also
observed between G/G, A/G or A/A genotypes and type 2 Diabetes. The frequency
of the ?T? allele of rs12255372 (G/T) was significantly associated with type
2 diabetes (OR= 0.55, 95% confidence interval [CI], 1.11-1.51; P<0.001). No
allelic association was found for rs7903146(C/T) polymorphism. The
distribution of alleles in case and control groups are significantly
different indicating the G allele is associated with type 2 diabetes. The
rs12255372 (G/T) may be associated with type 2 diabetes.
A novel 111/121 diplotype in the Calpain-10 gene is associated with type 2 diabetes
