A study on association of polymorphisms in calpain10 and TCF7l2 genes with type 2 diabetes mellitus

Polymorphisms of the Calpain10 and TCF7L2 genes were identified as possible type 2 diabetes susceptibility genetic markers. We conducted a case-control study to evaluate the relation between SNP43 of calpain-10 and rs12255372 and rs7903146 in the TCF7L2 with type2 diabetes in western-north of Iran. The role of these variants in Iranian population was less clear. A total of 202 patients and healthy controls were enrolled to analysis the frequency distribution of Calpain10 and TCF7L2 polymorphisms (SNP43, rs12255372 and rs7903146) using polymerase chain reaction-restriction fragment length polymorphism (PCR - RFLP) method. The frequency of allele A in controls was significantly greater than that of diabetic patients (P=0.031), whereas the difference between distribution of SNP43 genotypes (A/A, A/G, G/G) were non- significant in case and control groups. Non significant association was also observed between G/G, A/G or A/A genotypes and type 2 Diabetes. The frequency of the ?T? allele of rs12255372 (G/T) was significantly associated with type 2 diabetes (OR= 0.55, 95% confidence interval [CI], 1.11-1.51; P<0.001). No allelic association was found for rs7903146(C/T) polymorphism. The distribution of alleles in case and control groups are significantly different indicating the G allele is associated with type 2 diabetes. The rs12255372 (G/T) may be associated with type 2 diabetes.

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The Effectiveness of Logotherapy Training on Psychological Well-Being and Life Expectancy in Patients with Diabetes Type 2

Iranian journal of diabetes and obesity ◽

10.18502/ijdo.v13i4.7997 ◽

2021 ◽

Author(s):

Jafar Shabani ◽

Rafat Mahmoudi Tabar

Keyword(s):

Type 2 Diabetes ◽

Life Expectancy ◽

Well Being ◽

Analysis Of Covariance ◽

Diabetic Patients ◽

Control Groups ◽

Psychological Well Being ◽

Post Test ◽

And Control

Objective: In daily life, logotherapy is one of the skills that can be taught to increase the levels of psychological well-being and hope. The purpose of this study was to evaluate the effectiveness of meaningful psychological skills in promoting psychological well-being and life expectancy in patients with type 2 diabetes in the city of Salas city of Kermanshah province. Materials and Methods: The present study is an experiment with a pre-test-post-test design with a control group. The statistical population of the study was all patients with type 2 diabetes in Salas city of Kermanshah province. Thirty people were randomly divided into two groups of 15 experimental and controlled by sampling. After that, the experimental group was trained in logotherapy skills and meaning for 2 months in a total of 8 sessions of 90 minutes. After completing the training and after one month, the post-test was performed simultaneously and under the same conditions for both experimental and control groups. Data were analyzed by multivariate analysis of covariance (MANCOVA) with SPSS 18 software. Results: Results of analysis of covariance on post-test means, there is a significant difference between logotherapy and control groups in psychological well-being and life expectancy variables (P-value< 0.001). The value of squares indicates that about 77% of the variance of the well-being score and about 81% of the variance of the life expectancy score is explained by the difference between the two methods. Conclusion: Logotherapy training is effective on psychological well-being and life expectancy of diabetic patients.

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Ayurveda Body–Mind Constitutional Types and Role of Yoga Intervention Among Type 2 Diabetes Mellitus Population of Chandigarh and Panchkula Regions

Annals of Neurosciences ◽

10.1177/09727531211000040 ◽

2021 ◽

pp. 097275312110000

Author(s):

Madhava Sai Sivapuram ◽

Vinod Srivastava ◽

Navneet Kaur ◽

Akshay Anand ◽

Raghuram Nagarathna ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Lipid Profiles ◽

Control Groups ◽

Yoga Intervention ◽

Positive Effects ◽

Study Results ◽

Significant Difference ◽

And Control

Background: Type 2 diabetes needs a better understanding of etiological factors and management strategies based on lifestyle and constitutional factors, given its high association rate with many cardiovascular, neurological disorders, and COVID-19 infection. Purpose: The present study was undertaken to investigate the effect of Diabetes-specific integrated Yoga lifestyle Protocol (DYP) on glycemic control and lipid profiles of diabetic adults. Along with the DYP intervention, the individuals residing in Chandigarh and Panchkula union territories in the northern part of India were assessed for Ayurveda-based body–mind constitutional type. Ayurveda describes body–mind constitution as “ prakriti,” which has been discussed from two angles, namely physiological and psychological as body and mind are correlated. Methods: Cluster sampling of waitlist control study subjects was used as the sampling method for the study. A total of 1,215 registered subjects (81 diabetic) responded in randomly selected clusters in Chandigarh and Panchkula. Ayurveda physicians did Ayurveda body–mind constitutional assessment called prakriti assessment (physiological body–mind constitution assessment) in 35 participants (23 diabetic, 12 prediabetic) as a part of the study. Results: A group of 50 subjects was randomly selected for yoga intervention out of 81 diabetes mellitus adults, and 31 subjects were enrolled as waitlist controls. A significant decrease in the glycosylated hemoglobin levels from 8.49 ± 1.94% to 7.97 ± 2.20% in the intervention group was noticed. The lipid profiles of the DYP intervention and control groups were monitored. Three-month follow-up results of lipid profile diagnostic tests in intervention and control groups showed a significant difference between the two groups ( P < 0.05). Most diabetic and prediabetic individuals were found to have pitta dosha ( pitta controls all heat, metabolism, and transformation in the mind and body) as dominant constitution type. Conclusion: The study results demonstrated significant positive effects of yoga in diabetic individuals. This study has indicated the evidence for the safety and efficacy of the validated DYP for community-level interventions to prevent maladies like brain damage and stroke.

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Intermuscular Fat Content in Young Chinese Men With Newly Diagnosed Type 2 Diabetes: Based on MR mDIXON-Quant Quantitative Technique

Frontiers in Endocrinology ◽

10.3389/fendo.2021.536018 ◽

2021 ◽

Vol 12 ◽

Author(s):

Fuyao Yu ◽

Bing He ◽

Li Chen ◽

Fengzhe Wang ◽

Haidong Zhu ◽

...

Keyword(s):

Type 2 Diabetes ◽

Skeletal Muscle ◽

Fat Content ◽

Control Group ◽

Diabetic Patients ◽

Diabetes Group ◽

Control Subjects ◽

Intermuscular Fat ◽

And Control

ObjectiveSkeletal muscle fat content is one of the important contributors to insulin resistance (IR), but its diagnostic value remains unknown, especially in the Chinese population. Therefore, we aimed to analyze differences in skeletal muscle fat content and various functional MRI parameters between diabetic patients and control subjects to evaluate the early indicators of diabetes. In addition, we aimed to investigate the associations among skeletal muscle fat content, magnetic resonance parameters of skeletal muscle function and IR in type 2 diabetic patients and control subjects.MethodsWe enrolled 12 patients (age:29-38 years, BMI: 25-28 kg/m2) who were newly diagnosed with type 2 diabetes (intravenous plasma glucose concentration≥11.1mmol/l or fasting blood glucose concentration≥7.0mmol/l) together with 12 control subjects as the control group (age: 26-33 years, BMI: 21-28 kg/m2). Fasting blood samples were collected for the measurement of glucose, insulin, 2-hour postprandial blood glucose (PBG2h), and glycated hemoglobin (HbAlc). The magnetic resonance scan of the lower extremity and abdomen was performed, which can evaluate visceral fat content as well as skeletal muscle metabolism and function through transverse relaxation times (T2), fraction anisotropy (FA) and apparent diffusion coefficient (ADC) values.ResultsWe found a significant difference in intermuscular fat (IMAT) between the diabetes group and the control group (p<0.05), the ratio of IMAT in thigh muscles of diabetes group was higher than that of control group. In the entire cohort, IMAT was positively correlated with HOMA-IR, HbAlc, T2, and FA, and the T2 value was correlated with HOMA-IR, PBG2h and HbAlc (p<0.05). There were also significant differences in T2 and FA values between the diabetes group and the control group (p<0.05). According to the ROC, assuming 8.85% of IMAT as the cutoff value, the sensitivity and specificity of IMAT were 100% and 83.3%, respectively. Assuming 39.25ms as the cutoff value, the sensitivity and specificity of T2 value were 66.7% and 91.7%, respectively. All the statistical analyses were adjusted for age, BMI and visceral fat content.ConclusionDeposition of IMAT in skeletal muscles seems to be an important determinant for IR in type 2 diabetes. The skeletal muscle IMAT value greater than 8.85% and the T2 value greater than 39.25ms are suggestive of IR.

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Can hematological parameters in type 2 diabetes predict microvascular complication development?

Pakistan Journal of Medical Sciences ◽

10.12669/pjms.35.6.1150 ◽

2019 ◽

Vol 35 (6) ◽

Author(s):

Erhan Onalan ◽

Nevzat Gozel ◽

Emir Donder

Keyword(s):

Type 2 Diabetes ◽

Microvascular Complications ◽

Hematological Parameters ◽

Diabetic Patients ◽

Healthy Controls ◽

Neutrophil Lymphocyte Ratio ◽

Lymphocyte Ratio ◽

The Mean ◽

The Difference

Objective: To examine potential associations between neutrophil/lymphocyte ratio, platelet/lymphocyte ratio, mean platelet volume (MPV), HbA1c and microvascular complications in diabetic patients from a cost-effectiveness perspective. Methods: One hundred patients with type 2 diabetes attending our outpatient unit between May 2018 and October 2018 were included, and 100 healthy individuals served as the control group. A retrospective file search was performed to collect information on hemoglobin, mean platelet volume (MPV), glycosylated haemoglobin (HbA1c), hematocrit (Hct), neutrophil and lymphocyte count, neutrophil/lymphocyte ratio (NLR), platelets (Plt), platelet/lymphocyte ratio (PLR), and microvascular complications (neuropathy, retinopathy, nephropathy). Results: Demographic and laboratory data were retrospectively controlled between diabetes (n=100) and healthy control (n=100) groups. The mean age in diabetic patients and healthy controls was 56.34 and 36.68 years, respectively. The mean NLR in diabetics and healthy controls was 2.48 and 2.11, the difference in NLR being significant (p=0.002). MPV in diabetics and controls was 8.54 and 8.53, respectively, and the difference was not significant (p=0.93). PLR was also similar, i.e. 149.7 and 145.3 in diabetics and healthy controls (p=0.067). With respect to microvascular complications, retinopathy was found to be significantly associated with MPV and NLR (p=0.015, and p=0.051), and nephropathy showed a significant association with NLR (p=0.027) among diabetics. In contrast with the two other microvascular complications, no significant association between neuropathy and NLR could be detected, while PLR and neuropathy was significantly associated (p=0.003). Conclusion: Microvascular complications may be associated with certain hematologic parameters, as suggested by comparisons both between diabetics and healthy individuals and within the group of diabetic individuals. We believe that hematologic parameters such as hematocrit, MPV, NLR, and PLR, which can be obtained through a simple complete blood count, may be utilized as cost-effective predictors of diabetic microvascular complications. Further prospective studies with larger sample size are required to better delineate these associations. doi: https://doi.org/10.12669/pjms.35.6.1150 How to cite this:Onalan E, Gozel N, Donder E. Can hematological parameters in type 2 diabetes predict microvascular complication development? Pak J Med Sci. 2019;35(6):1511-1515. doi: https://doi.org/10.12669/pjms.35.6.1150 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Investigating ADAMTS7 and ADAMTS12 levels in prediabetic and Type 2 diabetic patients

Biomarkers in Medicine ◽

10.2217/bmm-2020-0161 ◽

2021 ◽

Author(s):

Mercan Taştemur ◽

Selvihan Beysel ◽

Sema Hepşen ◽

Sanem Öztekin ◽

Erman Çakal ◽

...

Keyword(s):

Inflammatory Markers ◽

Diabetic Patients ◽

Control Groups ◽

Type 2 Diabetic Patients ◽

Human Enzyme ◽

Significant Difference ◽

And Control ◽

Type 2 Diabetic

Background: This study aims to investigate the role of ADAMTS7 and ADAMTS12 on atherosclerosis and inflammation in prediabetic and diabetic patients. Patients & methods: Serum ADAMTS7 and ADAMTS12 levels were compared with the atherosclerotic and inflammatory markers in diabetic (n = 65, female 30.9%, mean age = 53 years), prediabetic (n = 55, female 36.6%, mean age = 49 years) and control groups (n = 55, females 32.5%, mean age = 49 years). Serum ADAMTS levels were determined by a human enzyme-liked immunoassay. Results: In terms of ADAMTS7, there was no significant difference between diabetic, prediabetic and control groups (50.93, 44.34, 59.07, respectively; p > 0.05). ADAMTS12 is lower in diabetics (p < 0.05), whereas it is similar in prediabetics and controls (14.53, 20.76, 25.05, respectively; p > 0.05). ADAMTS7 and ADAMTS12 levels did not differ in diabetic nephropathy, retinopathy and neuropathy (p > 0.05). Conclusion: While ADAMTS12 was significantly lower in diabetics and prediabetics, ADAMTS7 and ADAMTS12 were not related to diabetic complications (nephropathy, retinopathy and neuropathy).

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Metagenomic Analysis of Duodenal Microbiota Reveals a Potential Biomarker of Dysbiosis in the Course of Obesity and Type 2 Diabetes: A Pilot Study

Journal of Clinical Medicine ◽

10.3390/jcm9020369 ◽

2020 ◽

Vol 9 (2) ◽

pp. 369 ◽

Cited By ~ 5

Author(s):

Agnieszka Sroka-Oleksiak ◽

Agata Młodzińska ◽

Małgorzata Bulanda ◽

Dominika Salamon ◽

Piotr Major ◽

...

Keyword(s):

Type 2 Diabetes ◽

Genetic Factors ◽

Density Lipoprotein ◽

Duodenal Mucosa ◽

Gastrointestinal Microbiota ◽

Control Groups ◽

Potential Biomarker ◽

And Control ◽

Diabetes And Obesity

Numerous scientific studies confirm that, apart from environmental and genetic factors, a significant role is played by gastrointestinal microbiota in the aetiology of type 2 diabetes and obesity. Currently, scientists mainly focus on the distal intestinal microbiota, while the equally important proximal parts of the intestine are overlooked. The aim of the study was a qualitative analysis of the structure of the duodenal mucosa microbiota in groups of patients with obesity and with type 2 diabetes and where obesity qualified for bariatric surgery: sleeve gastrectomy. The microbiological results obtained were compared with some clinical parameters. As a result, it was possible to determine the microbiological core that the treatment and control groups had in common, including phyla: Firmicutes, Proteobacteria, and Actinobacteria. The patients with obesity and with type 2 diabetes and obesity presented a significantly lower number of genus Bifidobacterium compared to healthy subjects. Furthermore, the numbers of Bifidobacterium were positively correlated with the high density lipoprotein (HDL) concentration in the groups under study. The obtained results indicate that bacteria of the genus Bifidobacterium should be considered in the future in the context of a potential biomarker in the progress of type 2 diabetes and obesity.

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SERUM MAGNESIUM LEVEL IN DIABETIC RETINOPATHY - A CROSS SECTIONAL STUDY

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i5.257 ◽

2019 ◽

Vol 3 (5) ◽

Author(s):

Dr. I. Vijayapriya ◽

Dr. Prakash. S ◽

Dr. S. Hemadharshini

Keyword(s):

Type 2 Diabetes ◽

Diabetic Retinopathy ◽

Serum Magnesium ◽

Study Groups ◽

Control Group ◽

P Value ◽

Cross Sectional ◽

The Difference ◽

And Control

Background: Among different complications of diabetes, ddiabetic retinopathy is one of the leading causes of blindness in the world. Increase in the frequency of lower serum magnesium levels have been reported among patients with diabetes. Materials and methods: A total of 120 subjects were included in this study and divided into 3 groups. The study groups consisted of 40 patients that are type 2 diabetes with retinopathy and 40 patients with type 2 diabetes without retinopathy and control group consisted of 40 healthy subjects respectively. Both cases and controls were subjected to estimation of biochemical parameters. Results: Among the study population, (80) 66.67% participants were cases and another (40) 33.33% participants were controls. Among the people who had mild NPDR, the median Mg was 1.90 (IQR 1.80, 2.00). It was 1.90 (1.70, 2.00), 1.75 (1.67, 1.92), 1.8 (1.69, 2.0) and 2.10 (1.90, 2.20) among people with DM retinopathy moderate NPDR, severe NPDR, Proliferative retinopathy and no retinopathy respectively. The difference in the Mg across DM retinopathy was statistically significant (P Value 0.008). The difference between the values among both the case and control groups for certain parameters such as SBP, FBG, PPBG, HbA1c, Magnesium, Urea, and Creatinine were found to be statistically significant. Conclusion: Serum magnesium levels were significantly lower in patients with diabetic retinopathy compared to diabetics without complications and control group. Keywords: Diabetes mellitus, Diabetic retinopathy, Magnesium

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South Indian Cuisine with Low Glycemic Index Ingredients Reduces Cardiovascular Risk Factors in Subjects with Type 2 Diabetes

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17176232 ◽

2020 ◽

Vol 17 (17) ◽

pp. 6232

Author(s):

Nivedita Pavithran ◽

Harish Kumar ◽

Arun Somasekharan Menon ◽

Gopala Krishna Pillai ◽

Karimassery Ramaiyer Sundaram ◽

...

Keyword(s):

Risk Factors ◽

Type 2 Diabetes ◽

Cardiovascular Risk ◽

Glycemic Index ◽

Control Groups ◽

Randomized Controlled ◽

South Indian ◽

And Control ◽

Low Glycemic Index

Background: Inflammation is considered as a predictor of cardiovascular diseases in type 2 diabetes mellitus. No previous studies have investigated the effect of low glycemic index (LGI) recipes of South Indian cuisine on the risk factors of cardiovascular disease in patients with diabetes. Aim: The aim of this randomized controlled trial was to evaluate the improvement in cardiovascular risk factors and blood glucose control, in patients with type 2 diabetes, after intervention with recipes of Kerala cuisine, from locally available whole grain cereals, low in glycemic index. Method: This was a prospective and randomized controlled study that was conducted over a period of 24 weeks. A total of 80 participants were recruited from the Department of Endocrinology and Diabetes Outpatient in Kerala, South India. All 80 patients had type 2 diabetes, and were aged between 35 and 65 years. Participants were randomly assigned and advised to follow either a LGI diet plan (n = 40) or their usual diet, which served as a control group (n = 40). The advice was reinforced throughout the study period. Anthropometric, biochemical parameters which included glycemic and cardio-metabolic parameters were measured according to standard procedures. T-tests were conducted to compare the differences between intervention and control groups, and the Pearson correlation coefficient was used to evaluate associations between the variables. Results: There were significant differences (p < 0.05) between the intervention and control groups with respect to weight, HbA1c, insulin, triglycerides, Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), high sensitivity C-reactive protein (hs-CRP) and apolipoprotein B (ApoB). There was also a positive correlation between weight and blood glucose variables. ApoB was positively correlated with lipid profile and insulin levels. Conclusions: The long-term implementation of LGI diet of Kerala cuisine has been found to promote weight loss, enhance insulin sensitivity and reduce the cardiovascular risk.

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Myocardial insulin resistance associated with chronic hypertriglyceridemia and increased FFA levels in Type 2 diabetic patients

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00629.2003 ◽

2004 ◽

Vol 287 (3) ◽

pp. H1225-H1231 ◽

Cited By ~ 29

Author(s):

Lucilla D. Monti ◽

Claudio Landoni ◽

Emanuela Setola ◽

Elena Galluccio ◽

Pietro Lucotti ◽

...

Keyword(s):

Nitric Oxide ◽

Insulin Resistance ◽

Control Group ◽

Diabetic Patients ◽

Control Groups ◽

Type 2 Diabetic Patients ◽

Myocardial Insulin Resistance ◽

And Control ◽

Type 2 Diabetic

We evaluated the influence of chronic hypertriglyceridemia and endothelial dysfunction on myocardial glucose uptake (MGU) in Type 2 diabetic patients without coronary heart disease. Patients were divided into two groups according to fasting triglyceride (TG) levels: 5.4 ± 1.1 and 1.5 ± 0.3 mmol/l for high- and normal-TG groups, respectively. Five subjects were assigned to the high-TG group and 11 to the normal-TG group. Age, gender, body mass index, systolic and diastolic blood pressure, glucose, insulin, HbA1c, cholesterol, and HDL cholesterol were similar in the two groups, whereas free fatty acid (FFA) levels were higher in the high-TG group basally and at the end of the clamp. Furthermore, five healthy subjects were subjected to the same protocol and used as the control group. MGU was assessed by using 18F-labeled 2-fluoro-2-deoxy-d-glucose under hyperglycemic-hyperinsulinemic conditions. Basal endothelin-1 and nitric oxide levels were significantly higher in the high-TG group than in the normal-TG and control groups, and cGMP and maximal postischemic vasodilation were significantly decreased in the high-TG group compared with the normal-TG and control groups. However, significant alterations were found in the same parameters in the normal-TG group compared with the control group. By the end of the hyperglycemic clamp, in the high-TG group, MGU was ∼40 and 65% of that in the normal-TG and control groups. MGU negatively correlated with TG, FFA, and endothelin-1, whereas a positive correlation was found with cGMP and maximal postischemic vasodilation. In conclusion, increased TG and FFA levels are risks, in addition to Type 2 diabetes mellitus, for myocardial insulin resistance, endothelial dysfunction, and alteration of nitric oxide/cGMP levels.

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α-2-Macroglobulin in Saliva Is Associated with Glycemic Control in Patients with Type 2 Diabetes Mellitus

Disease Markers ◽

10.1155/2015/128653 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 7

Author(s):

Juan Pablo Aitken ◽

Carolina Ortiz ◽

Irene Morales-Bozo ◽

Gonzalo Rojas-Alcayaga ◽

Mauricio Baeza ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Glycemic Control ◽

Control Group ◽

Diabetic Patients ◽

Promising Alternative ◽

Positive Discrimination ◽

And Control

Background. Subjects with type 2 diabetes mellitus (DM2) require an adequate glycemic control to avoid diabetic complications. Currently, saliva biomarkers are used as a diagnostic tool and can be indicative of the degree of progression and control of various diseases. Several studies indicate thatα-2-macroglobulin levels are elevated in diabetic patients.Methods. 120 subjects with DM2 were enrolled and classified into two groups according to their glycemic control (percentage of glycated hemoglobin-A1c (HbA1c), <7% adequate glycemic control group; >7% inadequate glycemic control group). The relationship betweenα-2-macroglobulin levels from saliva samples and HbA1c was subsequently evaluated.Results. We found a positive correlation betweenα-2-macroglobulin and HbA1c (r=0.778andP<0.0001). Area under the receivers operating characteristic (ROC) curve ofα-2-macroglobulin indicated a positive discrimination threshold ofα-2-macroglobulin (AUC = 0.903, CI 95%: 0.847–0.959,P<0.0001) to diagnose glycemic control.Conclusions. Our data strongly suggest that the level of salivaα-2-macroglobulin is an indicator for the degree of glycemic control in diabetic patients and represents a promising alternative method to evaluate this parameter.

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