Comparison of E Test and Double Disk Diffusion Test for Detection of Extended Spectrum Beta-Lactamases

2002 ◽  
Vol 21 (3) ◽  
pp. 241-243 ◽  
Author(s):  
A. Florijn ◽  
S. Nijssen ◽  
F. Schmitz ◽  
J. Verhoef ◽  
A. Fluit
Keyword(s):  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases

scholarly journals Infections with Nontyphoidal Salmonella Species Producing TEM-63 or a Novel TEM Enzyme, TEM-131, in South Africa

2004 ◽  
Vol 48 (11) ◽  
pp. 4263-4270 ◽  
Author(s):  
Tersia Kruger ◽  
Dora Szabo ◽  
Karen H. Keddy ◽  
Kathleen Deeley ◽  
Jane W. Marsh ◽  
...  
Keyword(s):  
South Africa ◽  
Public Health Problem ◽  
Amino Acid Substitutions ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Significant Public Health Problem ◽  
Significant Public Health ◽  
Extended Spectrum Beta Lactamases

ABSTRACT Salmonella spp. producing extended-spectrum beta-lactamases (ESBLs) have been reported in many countries, but there is no information on their prevalence in Africa. ESBL-producing Salmonella enterica serotype Isangi and S. enterica serotype Typhimurium strains have been noted in South Africa since 2001. A total of 160 consecutive isolates of Salmonella spp. were collected from 13 hospitals located in different cities in South Africa over a 5-month period from December 2002 to April 2003. All strains were screened for production of ESBLs by the double disk diffusion test and for AmpC production by assessing resistance to cefoxitin. bla SHV, bla TEM, bla CTX-M, and bla CMY-2 were sought from all ESBL-positive and cefoxitin-resistant isolates. A total of 15.6% (25 of 160) isolates produced SHV or TEM ESBLs, and 1.9% (3 of 160) produced CMY-2. Nine S. enterica serotype Typhimurium, eight S. enterica serotype Isangi, and three S. enterica serotype Muenchen strains produced either TEM-63 or a derivative of TEM-63 designated TEM-131. Both TEM-63 and TEM-131 have an isoelectric point of 5.6, and their sequences have the following amino acid substitutions compared to the TEM-1 sequence: Leu21Phe, Glu104Lys, Arg164Ser, and Met182Thr. Additionally, TEM-131 has an Ala237Thr substitution. ESBL-producing Salmonella spp. have become a significant public health problem in South Africa with particular implications for the treatment of serious nontyphoidal Salmonella infections in children, for whom extended-spectrum cephalosporins were the preferred treatment.

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