Three new sensitive and specific heat-shock protein 70 PCRs for global Leishmania species identification

2011 ◽  
Vol 31 (7) ◽  
pp. 1453-1461 ◽  
Author(s):  
A. M. Montalvo ◽  
J. Fraga ◽  
I. Maes ◽  
J.-C. Dujardin ◽  
G. Van der Auwera
Cancer ◽  
2010 ◽  
Vol 116 (16) ◽  
pp. 3785-3796 ◽  
Author(s):  
Manoj Garg ◽  
Deepika Kanojia ◽  
Shikha Saini ◽  
Sushma Suri ◽  
Anju Gupta ◽  
...  

2010 ◽  
Vol 10 (2) ◽  
pp. 238-245 ◽  
Author(s):  
Jorge Fraga ◽  
Ana Margarita Montalvo ◽  
Simonne De Doncker ◽  
Jean-Claude Dujardin ◽  
Gert Van der Auwera

2013 ◽  
Vol 18 (30) ◽  
Author(s):  
G Van der Auwera ◽  
I Maes ◽  
S De Doncker ◽  
C Ravel ◽  
L Cnops ◽  
...  

We describe Leishmania species determination on clinical samples on the basis of partial sequencing of the heat-shock protein 70 gene (hsp70), without the need for parasite isolation. The method is especially suited for use in non-endemic infectious disease clinics dealing with relatively few cases on an annual basis, for which no fast high throughput diagnostic tests are needed. We show that the results obtained from this gene are in nearly perfect agreement with those from multilocus enzyme electrophoresis, which is still considered by many clinicians and the World Health Organization (WHO) as the gold standard in Leishmania species typing. Currently, 203 sequences are available that cover the entire hsp70 gene region analysed here, originating from a total of 41 leishmaniasis endemic countries, and representing 15 species and sub-species causing human disease. We also provide a detailed laboratory protocol that includes a step-by-step procedure of the typing methodology, to facilitate implementation in diagnostic laboratories.


2001 ◽  
Vol 120 (5) ◽  
pp. A152-A152
Author(s):  
H SUZUKI ◽  
S NAGAHASHI ◽  
M MIYAZAWA ◽  
M MORI ◽  
H NAGATA ◽  
...  

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