Rate of Infection of Ixodes ricinus Ticks with Borrelia burgdorferi Sensu Stricto, Borrelia garinii, Borrelia afzelii and Group VS116 in an Endemic Focus of Lyme Disease in Italy

1998 ◽  
Vol 17 (2) ◽  
pp. 90-94 ◽  
Author(s):  
M. Cinco ◽  
D. Padovan ◽  
R. Murgia ◽  
L. Poldini ◽  
L. Frusteri ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Endemic Focus ◽  
Ixodes Ricinus Ticks ◽  
Borrelia Burgdorferi Sensu Stricto

scholarly journals DNA Microarray Assessment of Putative Borrelia burgdorferi Lipoprotein Genes

2002 ◽  
Vol 70 (6) ◽  
pp. 3300-3303 ◽  
Author(s):  
Fang Ting Liang ◽  
F. Kenneth Nelson ◽  
Erol Fikrig
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Dna Microarray ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Genes Encoding ◽  
The Individual

ABSTRACT A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.

scholarly journals Decorin Binding by DbpA and B of Borrelia garinii, Borrelia afzelii, and Borrelia burgdorferi Sensu Stricto

2011 ◽  
Vol 204 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Jemiina Salo ◽  
Vuokko Loimaranta ◽  
Pekka Lahdenne ◽  
Matti K. Viljanen ◽  
Jukka Hytönen
Keyword(s):  
Borrelia Burgdorferi ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Borrelia Burgdorferi Sensu Stricto

scholarly journals Genetic Heterogeneity of Borrelia burgdorferi Sensu Lato in Ixodes ricinus Ticks Collected in Belgium

1998 ◽  
Vol 36 (11) ◽  
pp. 3352-3354 ◽  
Author(s):  
Marie-Christine Misonne ◽  
Georges Van Impe ◽  
Philippe P. Hoet
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Single Species ◽  
Sensu Stricto ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Ixodes Ricinus Ticks ◽  
Biogeographic Regions ◽  
Species Specific ◽  
Comparison Of The Results

Borrelia burgdorferi sensu lato (s.l.), the etiological agent of Lyme disease, is transmitted by the bite of Ixodes ricinus. Four hundred eighty-nine ticks, collected in four locations of a region of southern Belgium where Lyme disease is endemic, were examined for the presence of the spirochete. In a PCR test with primers that recognize a chromosomal gene of all strains, 23% of the ticks were found to be infected. The species B. burgdorferi s.l. comprises at least three pathogenic genomospecies, B. burgdorferi sensu stricto (s.s.),Borrelia garinii, and Borrelia afzelii, which could be distinguished in PCR tests with species-specific primers that correspond to distinct plasmid sequences. B. garinii was most prevalent (53% of infected ticks), followed by B. burgdorferi s.s. (38%) and B. afzelii (9%). Of the infected ticks, 40% were infected with a single species, 40% were infected with two species, and 5% were infected with all three species. For 15% of the ticks, the infecting species could not be identified. No difference in rates of prevalence was observed among the four locations, which had similar ground covers, even though they belonged to distinct biogeographic regions. A greater heterogeneity of spirochetal DNA in ticks than in cultured reference DNA was suggested by a comparison of the results of PCRs with two different sets of species-specific primer sequences.

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