Rate of infection ofIxodes ricinus ticks withBorrelia burgdorferi sensu stricto,Borrelia garinii,Borrelia afzelii and group VS116 in an endemic focus of Lyme disease in Italy

1998 ◽  
Vol 17 (2) ◽  
pp. 90-94 ◽  
Author(s):  
M. Cinco ◽  
D. Padovan ◽  
R. Murgia ◽  
L. Poldini ◽  
L. Frusteri ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Endemic Focus

scholarly journals DNA Microarray Assessment of Putative Borrelia burgdorferi Lipoprotein Genes

2002 ◽  
Vol 70 (6) ◽  
pp. 3300-3303 ◽  
Author(s):  
Fang Ting Liang ◽  
F. Kenneth Nelson ◽  
Erol Fikrig
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Dna Microarray ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Genes Encoding ◽  
The Individual

ABSTRACT A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.

scholarly journals Identification and Functional Characterization of Complement Regulator-Acquiring Surface Protein 1 of the Lyme Disease Spirochetes Borrelia afzelii and Borrelia garinii

2005 ◽  
Vol 73 (4) ◽  
pp. 2351-2359 ◽  
Author(s):  
Reinhard Wallich ◽  
Joseph Pattathu ◽  
Veronique Kitiratschky ◽  
Christiane Brenner ◽  
Peter F. Zipfel ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Binding Site ◽  
Binding Protein ◽  
Functional Characterization ◽  
Surface Protein ◽  
Factor H ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Complement Regulator

ABSTRACT Complement regulator-acquiring surface protein 1 (CRASP-1) is the dominant factor-H-like protein 1 (FHL-1)- and factor-H-binding protein of Borrelia burgdorferi and is suggested to contribute to persistence of the pathogen. The prototype CRASP-1 of B. burgdorferi sensu stricto (CRASP-1Bb) has been formerly characterized. As shown recently, serum-resistant Borrelia afzelii strains express a unique FHL-1 and factor H-binding protein, designated CRASP-1Ba. Here, we describe for the first time the isolation and functional characterization of the gene encoding the full-length CRASP-1Ba of 28 kDa, which, upon processing, is predicted to be 26.4 kDa. CPASP-1Ba of B. afzelii spirochetes is associated with a genetic locus encoding the orthologous gbb54 gene family that maps to the linear plasmid of approximately 54 kb. Ligand affinity blotting techniques demonstrate that both native and recombinant CRASP-1Ba molecules strongly bind to FHL-1 and much more weakly to factor H. The FHL-1 and factor-H-binding site in CRASP-1Ba is shown to be localized to a 12-amino-acid residue domain at the C terminus of the protein. For comparison, the corresponding cspA-like gene(s) of a serum-sensitive Borrelia garinii strain has also been cloned and characterized. Most notably, two CRASP-1-related B. garinii proteins were identified; however, both molecules bind only weakly to FHL-1 and not at all to factor H. The present identification of the binding site of CRASP-1Ba represents an important step forward in our understanding of the pathogenesis of Lyme disease and may be helpful to design therapeutic regimens to interfere with complement evasion strategies of human pathogenic Borrelia strains.

scholarly journals Decorin Binding by DbpA and B of Borrelia garinii, Borrelia afzelii, and Borrelia burgdorferi Sensu Stricto

2011 ◽  
Vol 204 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Jemiina Salo ◽  
Vuokko Loimaranta ◽  
Pekka Lahdenne ◽  
Matti K. Viljanen ◽  
Jukka Hytönen
Keyword(s):  
Borrelia Burgdorferi ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Borrelia Burgdorferi Sensu Stricto

scholarly journals Adaptation of Diverse Lyme Disease Spirochetes in a Natural Rodent Reservoir Host

2004 ◽  
Vol 72 (4) ◽  
pp. 2442-2444 ◽  
Author(s):  
Dania Richter ◽  
Birte Klug ◽  
Andrew Spielman ◽  
Franz-Rainer Matuschka
Keyword(s):  
Lyme Disease ◽  
Incubation Period ◽  
Sensu Stricto ◽  
Reservoir Host ◽  
Borrelia Afzelii ◽  
Wood Mice ◽  
Reservoir Hosts

ABSTRACT We compared the relative reservoir competence of European wood mice for two genospecies of Lyme disease spirochetes by analyzing susceptibility, intrinsic incubation period, and degree and duration of infectivity. Borrelia afzelii, specializing in particular reservoir hosts, is better adapted to those hosts than is the more generalist genospecies B. burgdorferi sensu stricto.

scholarly journals Identification of a New Borrelia Species among Small Mammals in Areas of Northern Spain Where Lyme Disease Is Endemic

2005 ◽  
Vol 71 (3) ◽  
pp. 1336-1345 ◽  
Author(s):  
Horacio Gil ◽  
Marta Barral ◽  
Raquel Escudero ◽  
Ana L. García-Pérez ◽  
Pedro Anda
Keyword(s):  
Lyme Disease ◽  
Small Mammals ◽  
Phylogenetic Analyses ◽  
Sensu Stricto ◽  
Cross Reactivity ◽  
Northern Spain ◽  
Borrelia Garinii ◽  
Questing Ticks ◽  
Reservoir Hosts

ABSTRACT The role of small mammals as reservoir hosts for Borrelia burgdorferi was investigated in several areas where Lyme disease is endemic in northern Spain. A low rate of infestation by Ixodes ricinus nymphs was found in the small mammal populations studied that correlated with the near-absence of B. burgdorferi sensu lato in 184 animals tested and with the lack of transmission of B. burgdorferi sensu lato to I. ricinus larvae that fed on them. In contrast, questing ticks collected at the same time and in the same areas were found to carry a highly variable B. burgdorferi sensu lato repertoire (B. burgdorferi sensu stricto, Borrelia garinii, Borrelia valaisiana, and Borrelia afzelii). Interestingly, the only isolate obtained from small mammals (R57, isolated from a bank vole) grouped by phylogenetic analyses with other Borrelia species but in a separate clade from the Lyme disease and relapsing fever organisms, suggesting that it is a new species. This new agent was widely distributed among small mammals, with infection rates of 8.5 to 12% by PCR. Moreover, a high seroprevalence to B. burgdorferi sensu lato was found in the animal sera, suggesting cross-reactivity between B. burgdorferi sensu lato and R57. Although small mammals do not seem to play an important role as reservoirs for B. burgdorferi sensu lato in the study area, they seem to be implicated in the maintenance of spirochetes similar to R57.

scholarly journals Species-Specific Plasmid Sequences for PCR Identification of the Three Species of Borrelia burgdorferiSensu Lato Involved in Lyme Disease

1998 ◽  
Vol 36 (1) ◽  
pp. 269-272 ◽  
Author(s):  
Marie-Christine Misonne ◽  
Philippe Pierre Hoet
Keyword(s):  
Lyme Disease ◽  
Pcr Amplification ◽  
Sensu Stricto ◽  
Pcr Assay ◽  
Borrelia Garinii ◽  
Specific Pcr ◽  
Pcr Identification ◽  
Identification Of Species ◽  
Species Specific ◽  
Specific Sequences

Species-specific sequences were shown to be carried by plasmids of the three main species of Borrelia burgdorferi sensu lato involved in Lyme disease. Libraries of the 16-, 33-, and 25-kb plasmids of B. burgdorferi sensu stricto, Borrelia garinii, and Borrelia afzelii, respectively, were then built and used to isolate species-specific sequences. After sequencing of the cloned inserts, three sets of primers were designed. They were shown to determine species-specific PCR amplification products. The sensitivities of the PCR assay with these primers were 100 spirochetes for B. burgdorferi sensu stricto and 1,000 spirochetes for B. garinii and B. afzelii. The usefulness of these primers for the identification of species in biological samples (tick, serum, and cerebrospinal fluid samples) was ascertained.

scholarly journals Whole-Genome Sequences of Two Borrelia afzelii and Two Borrelia garinii Lyme Disease Agent Isolates

2011 ◽  
Vol 193 (24) ◽  
pp. 6995-6996 ◽  
Author(s):  
S. R. Casjens ◽  
E. F. Mongodin ◽  
W.-G. Qiu ◽  
J. J. Dunn ◽  
B. J. Luft ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Whole Genome ◽  
Genome Sequences ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Disease Agent ◽  
Lyme Disease Agent
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