Green synthesis of bimetallic copper–silver nanoparticles and their application in catalytic and antibacterial activities

2019 ◽  
Vol 22 (1) ◽  
pp. 269-277 ◽  
Author(s):  
Jawhara Al-Haddad ◽  
Fatima Alzaabi ◽  
Priyabrata Pal ◽  
K. Rambabu ◽  
Fawzi Banat
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antibacterial Activities

Green Synthesis of Silver Nanoparticles Using Chenopodium aristatum L. Stem Extract and Their Catalytic/Antibacterial Activities

2016 ◽  
Vol 28 (3) ◽  
pp. 1319-1333 ◽  
Author(s):  
Chun-Gang Yuan ◽  
Can Huo ◽  
Bing Gui ◽  
Pengle Liu ◽  
Cheng Zhang
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antibacterial Activities ◽  
Stem Extract

Light-Mediated Green Synthesis of DNA-Capped Silver Nanoparticles and Their Antibacterial Activity

2020 ◽  
Vol 20 (3) ◽  
pp. 1678-1684
Author(s):  
Jiraporn Chumpol ◽  
Sineenat Siri
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Light Exposure ◽  
Low Cost ◽  
Reaction Times ◽  
Antibacterial Activities ◽  
Face Centered Cubic ◽  
Stabilizing Agents ◽  
Face Centered ◽  
Average Size

Green synthesis offers an eco-friendly and low-cost approach for the synthesis of silver nanoparticles (AgNPs). Many studies have reported on the use of biomolecules, especially plant extracts, as reducing and/or stabilizing agents in place of toxic chemicals. This study reports on the use of bacterial genomic DNA as an alternative stabilizing agent for the green synthesis of AgNPs under light activation. With both increased DNA quantities and reaction times under light exposure, more stabilized AgNPs formed as indicated by the surface plasmon resonance intensities. The synthesized AgNPs were spherical with an average size of 61.36±10.15 nm as calculated using the dynamic light scattering (DLS) technique. The X-ray diffraction, selected area electron diffraction, and high resolution transmission electron microscope (TEM) analyses confirmed the formation of face-centered cubic (fcc) structured AgNPs. The produced AgNPs exhibited antibacterial activities against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, suggesting its potential application as an antibacterial agent.

scholarly journals Green Synthesis and Antibacterial Activities of Silver Nanoparticles Using Extracellular Laccase of Lentinus edodes

2015 ◽  
Vol 7 (4) ◽  
pp. 405-411 ◽  
Author(s):  
Agbaje LATEEF ◽  
Adeyemi Ojutalayo ADEEYO
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Wild Strain ◽  
Antibacterial Activities ◽  
Sem Analysis ◽  
Lentinus Edodes ◽  
Visible Spectroscopy ◽  
Extracellular Laccase ◽  
Effective Inhibition ◽  
Uv Visible

This study reports the multi-step mutagenesis of Lentinus edodes towards optimization of the production of laccase and novel application of laccase in the biosynthesis of silver nanoparticles (AgNPs) which could be used to develop an eco-friendly method for the rapid biosynthesis of AgNPs. The wild strain of L. edodes was subjected to UV irradiation at 254 nm and the resultant viable mutant was further treated with acridine orange, a chemical mutagen. The strains were evaluated for the production of laccase and the crude laccase of the UV mutant (UV10) was used for the green synthesis of AgNPs. The particles were characterized by UV-Visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). Laccase activities of wild, UV10 and UV10ACR8 strains of L. edodes were obtained as 2.6, 10.6 and 2.8 U/ml/min respectively after 7 days of fermentation, showing laccase yield improvement of 4.08-fold for UV10 mutant. UV-Visible spectroscopy indicated the formation of AgNPs at absorption band of 430 nm. FTIR result indicated that proteins were responsible for AgNP synthesis, while SEM analysis confirmed the formation of walnut-shaped nanoparticles with size range of 50-100 nm. The biosynthesized nanoparticles revealed effective inhibition against clinical isolates of Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. To the best of the authors’ knowledge, this result represents the first report on the biosynthesis of AgNPs using L. edodes metabolite. The report adds to the growing relevance of L. edodes as potential industrially viable organism, used for diverse biotechnological applications.

scholarly journals Green Synthesis of Silver Nanoparticles with Culture Supernatant of a Bacterium Pseudomonas rhodesiae and Their Antibacterial Activity against Soft Rot Pathogen Dickeya dadantii

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2303 ◽  
Author(s):  
Afsana Hossain ◽  
Xianxian Hong ◽  
Ezzeldin Ibrahim ◽  
Bin Li ◽  
Guochang Sun ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Sweet Potato ◽  
Culture Supernatant ◽  
Soft Rot ◽  
Antibacterial Activities ◽  
Simple Method ◽  
Dickeya Dadantii ◽  
Rot Disease

Bacterial stem and root rot disease of sweet potato caused by Dickeya dadantii recently broke out in major sweet potato planting areas in China and calls for effective approaches to control the pathogen and disease. Here, we developed a simple method for green synthesis of silver nanoparticles (AgNPs) using bacterial culture supernatants. AgNPs synthesized with the cell-free culture supernatant of a bacterium Pseudomonas rhodesiae displayed the characteristic surface plasmon resonance peak at 420–430 nm and as nanocrystallites in diameters of 20–100 nm determined by transmission electron microscopy, scanning electron microscopy, and X-ray diffraction spectroscopy. Functional groups associated with proteins in the culture supernatant may reduce silver ions and stabilize AgNPs. The AgNPs showed antibacterial activities against D. dadantii growth, swimming motility, biofilm formation, and maceration of sweet potato tubers whereas the culture supernatant of P. rhodesiae did not. AgNPs (12 µg∙ml−1) and AgNO3 (50 µg∙ml−1) showed close antibacterial activities. The antibacterial activities increased with the increase of AgNP concentrations. The green-synthesized AgNPs can be used to control the soft rot disease by control of pathogen contamination of sweet potato seed tubers.

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