Light-Mediated Green Synthesis of DNA-Capped Silver Nanoparticles and Their Antibacterial Activity

2020 ◽  
Vol 20 (3) ◽  
pp. 1678-1684
Author(s):  
Jiraporn Chumpol ◽  
Sineenat Siri
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Light Exposure ◽  
Low Cost ◽  
Reaction Times ◽  
Antibacterial Activities ◽  
Face Centered Cubic ◽  
Stabilizing Agents ◽  
Face Centered ◽  
Average Size

Green synthesis offers an eco-friendly and low-cost approach for the synthesis of silver nanoparticles (AgNPs). Many studies have reported on the use of biomolecules, especially plant extracts, as reducing and/or stabilizing agents in place of toxic chemicals. This study reports on the use of bacterial genomic DNA as an alternative stabilizing agent for the green synthesis of AgNPs under light activation. With both increased DNA quantities and reaction times under light exposure, more stabilized AgNPs formed as indicated by the surface plasmon resonance intensities. The synthesized AgNPs were spherical with an average size of 61.36±10.15 nm as calculated using the dynamic light scattering (DLS) technique. The X-ray diffraction, selected area electron diffraction, and high resolution transmission electron microscope (TEM) analyses confirmed the formation of face-centered cubic (fcc) structured AgNPs. The produced AgNPs exhibited antibacterial activities against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, suggesting its potential application as an antibacterial agent.

scholarly journals Green Synthesis and Catalytic Activity of Silver Nanoparticles Based on Piper chaba Stem Extracts

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1777 ◽  
Author(s):  
Md. Mahiuddin ◽  
Prianka Saha ◽  
Bungo Ochiai
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Ftir Spectroscopy ◽  
Visual Observation ◽  
Face Centered Cubic ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Size

A green synthesis of silver nanoparticles (AgNPs) was conducted using the stem extract of Piper chaba, which is a plant abundantly growing in South and Southeast Asia. The synthesis was carried out at different reaction conditions, i.e., reaction temperature, concentrations of the extract and silver nitrate, reaction time, and pH. The synthesized AgNPs were characterized by visual observation, ultraviolet–visible (UV-vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), energy dispersive x-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. The characterization results revealed that AgNPs were uniformly dispersed and exhibited a moderate size distribution. They were mostly spherical crystals with face-centered cubic structures and an average size of 19 nm. The FTIR spectroscopy and DLS analysis indicated that the phytochemicals capping the surface of AgNPs stabilize the dispersion through anionic repulsion. The synthesized AgNPs effectively catalyzed the reduction of 4-nitrophenol (4-NP) and degradation of methylene blue (MB) in the presence of sodium borohydride.

scholarly journals Green Synthesis of Silver Nanoparticles Using Bilberry and Red Currant Waste Extracts

Processes ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 193 ◽  
Author(s):  
Antonio Zuorro ◽  
Annalaura Iannone ◽  
Stefano Natali ◽  
Roberto Lavecchia
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Hydrodynamic Diameter ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Stabilizing Agents ◽  
Resonance Band ◽  
Effects Of Temperature ◽  
Face Centered ◽  
Red Currant

The production of silver nanoparticles (Ag-NPs) from bilberry waste (BW) and red currant waste (RCW) extracts was studied. Red fruit extracts were obtained by treating BW and RCW with aqueous ethanol (50% v/v) at 40 °C. The formation of nanoparticles was monitored spectrophotometrically by measuring the intensity of the surface plasmon resonance band (SPR) of silver. The effects of temperature (20–60 °C) and pH (8–12) on the reaction kinetics and on the properties of Ag-NPs were investigated. Characterization by XRD and dynamic light scattering (DLS) techniques showed that Ag-NPs were highly crystalline, with a face-centered cubic structure and a hydrodynamic diameter of 25–65 nm. The zeta potential was in the range of −35.6 to −20.5 mV. Nanoparticles obtained from BW were slightly smaller and more stable than those from RCW. A kinetic analysis by the initial-rate method showed that there was an optimum pH, around 11, for the production of Ag-NPs. Overall, the results obtained suggest that BW and RCW can be advantageously used as a source of reducing and stabilizing agents for the green synthesis of Ag-NPs.

Development of Antibacterial Xanthan/Chitosan Biguanidine Hydrochlo ride Polyelectrolyte Complexes Decorated with Eco-friendly Prepared Silver Nanoparticles

2020 ◽  
Vol 10 ◽  
Author(s):  
Hend Ezzat Salama ◽  
Mohamed Samir Abdel Aziz
Keyword(s):  
Silver Nanoparticles ◽  
Biomedical Applications ◽  
Decomposition Temperature ◽  
Homogeneous Distribution ◽  
Face Centered Cubic ◽  
Polyelectrolyte Complexes ◽  
X Ray ◽  
Integral Procedure ◽  
Face Centered ◽  
Average Size

Background:: Novel eco-friendly silver nanocomposites of xanthan/chitosan biguanidine hydrochloride polyelectrolyte complexes were successfully prepared. Methods:: Silver nanoparticles (AgNPs) were formed through an insitu eco-friendly reduction by the non-toxic polysaccharides without the usage of toxic reagents. FTIR confirmed the successful preparation of the nanocomposites while XRD confirmed the presence of AgNPs with face-centered cubic structures. TEM confirmed the homogeneous distribution of AgNPs with an average size of 14.1 nm. SEM was used to study the surface morphology of the nanocomposites while the energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of AgNPs. Results:: Thermogravimetric analysis showed that the thermal stability was improved in the presence of AgNPs as detected from the calculated integral procedure decomposition temperature. Antibacterial activity against different bacteria species was significantly improved upon increasing the content of AgNPs. Conclusion:: Due to their interesting properties, the prepared polyelectrolyte complexes and their AgNPs nanocomposites could be employed potentially in many biomedical applications like drug delivery.

Green Synthesis of Silver Nanoparticles using the Leaves Extract of Filicium decipiens and its Anti- Microbial Activity

2021 ◽  
Vol 10 (3) ◽  
pp. 16-24
Author(s):  
Sherin Monichan ◽  
P. Mosae Selvakumar ◽  
Christine Thevamithra ◽  
M. S. A. Muthukumar Nadar ◽  
Jesse Joel
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Average Diameter ◽  
Diffusion Method ◽  
Face Centered Cubic ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Almost All

Silver nanoparticles has been used since ages, even till now it is exploited in almost all areas like medicine, textiles, industries, cosmetics, purification, dying and many more. There are many approaches which are used to synthesize silver nanoparticles. However, these approaches are either harmful to the environment or very costly. Therefore, green synthesis of silver nanoparticles (AgNPs) using leaves of Filicium decipiens eco-friendly and a very reliable method to procure AgNPs. Characterization of synthesized AgNPs were then done using UV-Vis spectroscopy and fluorescence which confirmed the formation of AgNPs, scanning electron microscope (SEM)confirmed its shape to be round and X-ray diffraction (XRD) determined its crystalline nature as face centered cubic structure. Furthermore, Dynamic Light Scattering (DLS) was also done in order to know the average diameter and zeta potential of AgNPs. However, it did not show potential results due to the aggregates formed during the green synthesis of AgNPs. In addition to this, anti-microbial test against bacteria such as gram negative (Escherichia. Coli) and gram positive (Bacillus.spc) were done using well-diffusion method and also its application of antimicrobial activity was tested over fabric to understand its application in textile industries. In both the cases, AgNPs showed more efficiency in gram negative bacteria than gram- positive.

Trillium govanianum Wall. Ex. Royle rhizomes extract-medicated silver nanoparticles and their antimicrobial activity

2020 ◽  
Vol 9 (1) ◽  
pp. 503-514 ◽  
Author(s):  
Khaleeq Uz-Zaman ◽  
Jehan Bakht ◽  
Bates Kudaibergenova Malikovna ◽  
Eman R. Elsharkawy ◽  
Anees Ahmed Khalil ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cost Effective ◽  
Face Centered Cubic ◽  
Synthesis Of Nanoparticles ◽  
E Coli ◽  
Area Of Interest ◽  
Cost Effective Technique ◽  
Face Centered ◽  
Trillium Govanianum ◽  
Average Size

AbstractSynthesis of nanoparticles is a fast-growing area of interest in the current development in science and technology. Nanoparticles are also used in biomedical applications. Green synthesis of nanoparticles is an environmental friendly and cost-effective technique. Trillium govanianum Wall. Ex. Royle crude extract was used for the eco-friendly genesis of silver nanoparticles (AgNPs). Aromatic amines were the functional groups involved in the bio-fabrication and synthesis of the AgNPs. The production of AgNPs was established by the appearance of brown color. The manufactured AgNPs were characterized by UV-Vis spectrophotometer, X-ray diffractometer, and FTIR spectrophotometer. AgNPs were face-centered cubic in nature with an average size of 9.99 nm. The produced AgNPs (18 µL disc−1) showed substantial antibacterial (53.74, 52.75, 51.61, 43.00, 36.84, and 36.84%) and antifungal (54.05, 42.11, 41.10, 40.85, 30.55, and 29.73%) potential against the tested bacterial (X. campestris, P. aeruginosa, S. aureus, E. coli, B. subtilis, and K. pneumoniae) and fungal (A. alternaria, Paecilomyces, C. albicans, Curvularia, A. niger, and Rhizopus) strains, respectively.

scholarly journals BIOREDUCTION AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM OIL PALM EMPTY FRUIT BUNCH

2020 ◽  
Vol 21 (4) ◽  
pp. 177
Author(s):  
Siti Suhartati ◽  
Iwan Syahjoko Saputra ◽  
Dwinna Rahmi ◽  
Yoki Yulizar ◽  
Sudirman Sudirman
Keyword(s):  
Silver Nanoparticles ◽  
Oil Palm ◽  
Average Particle Size ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Empty Fruit Bunch ◽  
The Face ◽  
Face Centered ◽  
Average Size

BIOREDUCTION AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM OIL PALM EMPTY FRUIT BUNCH (OPEFB). The synthesis of silver nanoparticles was successfully carried out by extracting oil palm empty fruit bunch. The precursor used was silver nitrate (AgNO3) with a concentration of 9x10-4 M and 5 wt% of the oil palm empty fruit bunch extract. OPEFB acted as a capping agent in the synthesis of silver nanoparticles. The bioreduction method Ag+ to Ag0 produced a silver nanoparticle colloid in brown color. The results of the UV-Vis spectrophotometer showed the silver nanoparticles colloids spectrum at a wavelength of 420 nm with an absorbance value of 0.5. FTIR shows the reduction and shift of absorption peak in the hydroxyl functional group (-OH) at wavenumbers of 3323 cm-1 and the presence of absorption peaks at 560 cm-1. While, XRD pattern showed the specific crystallinity peaks of silver nanoparticles at 2θ: 33.24°; 39.98°; 61.23°; dan 79.13° respectively with the face-centered cubic crystal structure (FCC) and crystallite size of 15 nm. PSA analysis showed two specific peaks with an average size distribution silver nanoparticles of 43.5 nm and a PDI value of 0.4. Analysis of TEM shows the average particle size of 20 nm with a spherical particle shape.

Anticancer and Antibacterial Activities of Silver Nanoparticles (AgNPs) Synthesized from Cucumis melo L.

2020 ◽  
Vol 20 (7) ◽  
pp. 4143-4151 ◽  
Author(s):  
Yun Wang ◽  
Xudong Zhang ◽  
Yuzhuo Bai ◽  
Wei Li ◽  
Xu Li ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Viability ◽  
Cucumis Melo ◽  
Xrd Analysis ◽  
Antibacterial Activities ◽  
Biological Evaluation ◽  
Face Centered Cubic ◽  
X Ray ◽  
Biosynthesized Agnps ◽  
Average Size

The current investigation reports the structural and biological evaluation of silver nanoparticles (AgNPs) biosynthesized from the pericarp extract of Cucumis melo L. (muskmelon). The AgNPs were characterized by ultraviolet-visible (UV-Vis) spectrophotometry, XRD (X-ray diffraction), SEM (scanning electron microscopy) and EDAX (energy-dispersive X-ray spectroscopy). The XRD analysis showed that biosynthesized AgNPs were having FCC (face centered cubic) crystalline structures. Further, the SEM and EDAX showed spherically shaped AgNPs having an average size of 25 nm. The AgNPs effectively inhibited the growth of Bacillus subtilis and Escherichia coli. Moreover, the cytotoxic assay of AgNPs revealed effective cytotoxicity against different cancer cells, such as HeLa, HCT-116, PC-3 and Jurkat in a dose reliant way. The cell viability was noticed to range from 50% to 60% with IC50 values ranging from 150 μg/mL to 224 μg/mL. The lower cell viability indicates the toxic effects of biosynthesized AgNPs against these malignant cells. Thus, the current study shows that these biosynthesized AgNPs could be utilized in various medical applications in near future.

Green Synthetic Nanoarchitectonics of Gold and Silver Nanoparticles Prepared Using Quercetin and Their Cytotoxicity and Catalytic Applications

2020 ◽  
Vol 20 (5) ◽  
pp. 2781-2790 ◽  
Author(s):  
You Jeong Lee ◽  
Youmie Park
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Agents ◽  
Biologically Active ◽  
Face Centered Cubic ◽  
Gold And Silver Nanoparticles ◽  
Transmission Electron ◽  
Nitrophenol Reduction ◽  
Face Centered ◽  
Cytotoxicity Assessment ◽  
Average Size

Quercetin is a flavonoid and is abundant in the plant kingdom. Green nanoparticles (gold and silver) were synthesized by using quercetin as a reductant via a green route for their potential nanoarchitechtonic applications. There were no toxic chemicals involved during the synthesis. The gold and silver nanoparticles exhibited surface plasmon resonance at 527 nm and 401 nm, respectively. Both nanoparticle solutions retained excellent colloidal shelf stability for 7 days and in cell culture medium. The crystal structure of the nanoparticles was observed by X-ray diffraction analysis. Field emission transmission electron microscopy images revealed that spherical nanoparticles were synthesized, with an average size of 20.2±4.8 nm for gold nanoparticles and 32.4±14.0 nm for silver nanoparticles. Observation of clear lattice fringes in the microscopic images suggested that both types of nanoparticles possessed a face-centered cubic structure. Catalytic activity was evaluated with respect to 4-nitrophenol reduction and methyl orange degradation. When increasing the amount of gold or silver nanoparticles used as a catalyst, the rate constant of the catalytic reaction was also increased. Cytotoxicity assessment on cancer cells demonstrated that both types of nanoparticles can be appropriate candidates for delivery vehicles of biologically active molecules, such as anticancer agents.

scholarly journals The Performance of p-Aminosalicylic Acid As Reducing and Stabilizing Agent in Silver Nanoparticles Synthesis

2019 ◽  
Vol 35 (1) ◽  
pp. 56-63 ◽  
Author(s):  
Dian Susanthy ◽  
Sri Juari Santosa ◽  
Eko Sri Kunarti
Keyword(s):  
Silver Nanoparticles ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Face Centered Cubic ◽  
Aminosalicylic Acid ◽  
Stabilizing Agent ◽  
Optimum Synthesis ◽  
Optimum Reaction ◽  
Face Centered ◽  
Average Size

In this study, silver nanoparticles (AgNPs) were successfully synthesized using p-aminosalicylic acid as a reducing and stabilizing agent simultaneously. The AgNPs was synthesized by mixing silver nitrate solution as a precursor with the pH adjusted by p-aminosalicylic acid solution and heating it in a boiling water bath. The formed AgNPs were analyzed using UV-Vis spectrophotometry to evaluate their SPR absorbance in the wavelength range of 400-500 nm. The optimum reaction time is 10 min and the optimum pH is 11. The AgNPs with the optimum synthesis condition have average size of 32.3 nm when characterized using PSA, spherical morphology when characterized using TEM, and face-centered cubic crystal when chara­cterized using XRD. The formed AgNPs had good stability for more than 2 months. The mechanism of silver ion reduction and AgNPs stabilization by p-aminosalicylic acid were also proposed in the paper based on the FTIR analysis result.

scholarly journals Biosynthesis of Silver Nanoparticles Using Terminalia Bellirica Fruit Extract and Their Characterization

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Mahesh Chandra Purohit ◽  
Rashmi Rawat ◽  
Anuj Kandwal ◽  
Aditya Ram Semwal ◽  
Rakesh Kumar Joshi
Keyword(s):  
Silver Nanoparticles ◽  
Ag Nanoparticles ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
Fruit Extracts ◽  
Agno3 Solution ◽  
Light Yellow ◽  
Terminalia Bellirica ◽  
Face Centered ◽  
Average Size

Green synthesis is an appropriate method for the synthesis of Ag nanoparticles using Terminalia Bellirica fruit extracts. The change in color from light yellow to dark brown was observed by adding AgNO3 solution into fruit extracts. The Ag nanoparticles were characterized by UV-Vis (Ultraviolet-visible), XRD, TEM and FTIR. The reduction of Ag + into Ag 0 was observed by UV-Vis spectrophotometer. The silver nanoparticles were found to be crystalline in nature when observed in the X-Ray Diffraction analysis. The geometry of these silver nanoparticles is face centered cubic geometry. When observed in the Transmission Electron Microscopy (TEM) analysis, these synthesized nanoparticles were found spherical in shape where their average size was less than 26 nanometers. FTIR peaks confirms the presence of phytochemicals which are responsible for reducing, capping and stabilizing the nanoparticles.

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