scholarly journals In situ and in vitro comparison of laser fluorescence with visual inspection in detecting occlusal caries lesions

2009 ◽  
Vol 26 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Andréia Bolzan de Paula ◽  
Juliana Álvares Duarte Bonini Campos ◽  
Michele Baffi Diniz ◽  
Josimeri Hebling ◽  
Jonas Almeida Rodrigues
2009 ◽  
Vol 43 (5) ◽  
pp. 405-412 ◽  
Author(s):  
M.M. Braga ◽  
F.M. Mendes ◽  
S. Martignon ◽  
D.N.J. Ricketts ◽  
K.R. Ekstrand

2006 ◽  
Vol 30 (4) ◽  
pp. 306-309 ◽  
Author(s):  
Renata Maciel e Rocha Cabral ◽  
Fausto Medeiros Mendes ◽  
José Nicolau ◽  
Denise Maria Zezell

The goal of this study was to evaluate the influence of a PVC Seal Wrap as barrier protection and the influence of autoclaving on the in vitro performance of a laser fluorescence device in detecting occlusal caries lesions in primary teeth. The clinical significance of the experiment is to create a protocol to avoid the possibility of underestimating caries in clinical setting.


2008 ◽  
Vol 24 (4) ◽  
pp. 501-506 ◽  
Author(s):  
Jonas A. Rodrigues ◽  
Michele B. Diniz ◽  
Érika B. Josgrilberg ◽  
Rita C. L. Cordeiro

2014 ◽  
Vol 8 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Diana Mortensen ◽  
Katrine Dannemand ◽  
Svante Twetman ◽  
Mette Kirstine Keller

Objective: To evaluate the performance of an impedance spectroscopy technology for detecting non-cavitated occlusal caries lesions in permanent teeth in vitro. The method was compared with a commonly used laser fluorescence device and validated against histology. Material and Methodology: A non-cavitated sample of 100 extracted posterior teeth was randomly selected and assessed for caries on enamel and dentin level with aid of CarioScan PRO (ACIS) and DIAGNOdent pen (LF pen) by three examiners. After the measurements, the extension of the lesion was histologically determined as gold standard. Sensitivity, specificity, accuracy and receiver-operating curves were calculated. Intra- and inter-examiner reproducibility was expressed by intra class correlation coefficients. Results: The histological caries prevalence was 99% and 41% exhibited dentin caries. The ACIS technique displayed high specificities but almost negligible sensitivities at readings >50. A similar pattern was noted for the LF pen at readings >30. The intra- and inter-examiner reproducibility varied between 0.47 and 0.98 and the values were generally lower for the ACIS technique than for the LF pen. The inter-examiner agreement reached excellent levels with both methods. Conclusions: In vitro, the ACIS technique showed a low ability to disclose occlusal caries lesions in the enamel and/or dentin of non-cavitated permanent molars. However, further in vivo studies of permanent occlusal surfaces are needed to mirror the clinical situation.


2014 ◽  
Vol 15 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Adriana Bona Matos ◽  
Bruna Uglik Garbui ◽  
Cynthia Soares de Azevedo ◽  
Caroline Martins e Silva ◽  
Maria Regina Lorenzetti Simionato ◽  
...  

ABSTRACT Aim This study evaluated and improved a protocol for obtaining standard caries-affected dentin (CAD) by Streptococcus mutans biofilm demineralization process. Materials and methods Forty-eight human molars were divided in six experimental groups, according to: period of cariogenic challenge (7, 14 or 21 days) and type of dentin (erupted or unerupted teeth). After complete cariogenic challenge sound and CAD dentin were evaluated by: visual inspection (VI), digital radiography (DR), optical coherence tomography (OCT) and laser fluorescence (LF). Results Visual inspection confirmed the formation of CAD based on tissue yellowing and loss of surface gloss. Digital radiography detected the presence of radiolucent images, suggesting caries. Three calibrated examiners viewed all images obtained by VI and DR and were able to distinguish healthy from CAD. Fisher's exact statistical test (p < 0.05) confirmed no difference between groups by VI (G1/G4: p = 0.6; G2/G5: p = 1; G3/G6: p = 1) or DR (G1/G4: p = 1; G2/G5: p = 1; G3/G6: p = 1). Both LF values and demineralization depth, as determined by OCT, were subjected to ANOVA (p < 0.05). For LF, a statistically significant difference was observed for the type of substrate (p = 0.001). For OCT, no statistically significant differences in the type of substrate (p = 0.163), length of cariogenic challenge (p = 0.512) or interaction between factors (p = 0.148) were observed. Scanning electron micrographs confirmed the presence of CAD; a more uniform demineralization surface was observed in the dentin of unerupted teeth. Conclusion This protocol suggests that standard CAD can be obtained in 7 days of cariogenic challenge using unerupted teeth. Clinical significance With the new perspective on the clinical treatment of caries lesions, bonding is increasingly performed to demineralize CAD, which is susceptible to remineralization. A useful protocol to standardize the production of CAD, by microbiological cariogenic challenge, would be an important contribution to laboratorial test in the field of operative dentistry. How to cite this article Azevedo CS, Garbui BU, Silva CM, Simionato MRL, Freitas AZ, Matos AB. Obtaining Artificially Caries-affected Dentin for in vitro Studies. J Contemp Dent Pract 2014;15(1):12-19.


1998 ◽  
Vol 32 (5) ◽  
pp. 324-329 ◽  
Author(s):  
M.-Ch. D.N.J.M. Huysmans ◽  
Ch. Longbottom ◽  
N.B. Pitts

Folia Medica ◽  
2020 ◽  
Vol 62 (2) ◽  
pp. 358-364
Author(s):  
Veselina Todorova ◽  
Ivan Filipov ◽  
Reneta Petrova

Introduction: Initial proximal caries is both diagnostic and therapeutic challenge. The disadvantages of the conventional methods for caries detection and the development of technologies led to the creation of contemporary optical devices for early caries detection. Aim: In vitro comparison of the diagnostic accuracy of several methods for early proximal caries detection &ndash; visual-tactile, bitewing radiography and laser fluorescence device (DIAGNOdent pen). Materials and methods: Fifty-eight proximal surfaces of extracted human permanent premolars and molars were examined by two examiners using visual inspection, bitewing radiography, DIAGNOdent with proximal contact, and DIAGNOdent directly in the lesion. Results were compared with the histological gold standard. Statistical analysis with ROC curve, sensitivity, specificity and diagnostic accuracy of each detection method was performed. Analysis was conducted in 3 diagnostic thresholds &ndash; initial, developed and advanced demineralization. Results: Sensitivity of visual inspection was 16%&ndash;33%, specificity 93.3%&ndash;100%, sensitivity of bitewing radiography 54%&ndash;67%, speci&shy;ficity 93%&ndash;94%, sensitivity of DIAGNOdent with proximal surfaces in contact 88%&ndash;91%, specificity 79%&ndash;89%, sensitivity of DIAG&shy;NOdent directly 89%&ndash;92.5%, specificity 81.29%&ndash;93%. The highest diagnostic accuracy, increasing with the rise of the level of demin&shy;eralization, was shown by DIAGNOdent directly, followed by DIAGNOdent with proximal contact, bitewing radiography, and visual inspection with the lowest accuracy. Conclusion: The use of contemporary diagnostic devices significantly increases the possibility for early detection of proximal lesions. DIAGNOdent can be used as an adjunct to and increasing the diagnostic accuracy of the conventional caries detection methods.


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