Optimization of Factors Affecting In vitro Establishment, Ex vitro Rooting and Hardening for Commercial Scale Multiplication of Silk Banana (Musa AAB)

2015 ◽  
Vol 57 (3) ◽  
pp. 153-164 ◽  
Author(s):  
A. A. Waman ◽  
Pooja Bohra ◽  
B. N. Sathyanarayana ◽  
K. Umesha ◽  
G. K. Mukunda ◽  
...  
Keyword(s):  
Ex Vitro Rooting ◽  
Ex Vitro ◽  
Factors Affecting ◽  
Commercial Scale ◽  
In Vitro Establishment

scholarly journals Factors Affecting Ex vitro Rooting in Micropropagated Shoots from Nodal Explants of Terminalia arjuna

2020 ◽  
pp. 92-97
Author(s):  
Meena Choudhary ◽  
Inder Dev Arya ◽  
Sarita Arya
Keyword(s):  
Large Scale ◽  
Incubation Temperature ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Terminalia Arjuna ◽  
Ex Vitro ◽  
Important Species ◽  
Factors Affecting ◽  
In Vitro Shoot Proliferation

The present work was done with the aim to study the effect of rooting mixture and incubation temperature on Ex vitro rooting of Terminalia arjuna, an important multipurpose tree. The nodal explant collected from Ummaid garden Jodhpur was subjected for In vitro shoot proliferation on BAP supplemented modified MS medium. These shoots were In vitro multiplied on BAP (half concentration of BAP used in In vitro shoot proliferation) with low concentration of NAA supplemented medium. The individual shoots from In vitro multiplied shoots were pulse treated with IBA for 10 min. and transferred in different rooting mixture and incubation temperature for Ex vitro rooting. Analysis of data revealed that maximum 62.22% rooting was observed when the plantlet pulse treated with 984.25 µM IBA for 10 min were transferred on bottle containing vermiculite as rooting mixture and incubated at the temperature of 26°C.The optimization of Ex vitro rooting mixture and temperature conditions will be helpful in propagation of this important species rapidly in large scale.

scholarly journals Ex Vitro Rooting of American Chestnut Improves Acclimatization Survival and Plantlet Quality

2016 ◽  
Vol 34 (3) ◽  
pp. 75-79 ◽  
Author(s):  
Allison D Oakes ◽  
Tyler R. Desmarais ◽  
William A. Powell ◽  
Charles A. Maynard
Keyword(s):  
Tissue Culture ◽  
Keystone Species ◽  
Ex Vitro Rooting ◽  
Plant Production ◽  
Shoot Cultures ◽  
Ex Vitro ◽  
Regulatory Review ◽  
Survival Percentage ◽  
Virus Indexing

Tissue culture of plants has many applications, from producing genetically identical horticultural varieties, to production of secondary metabolites, to virus indexing, and most relevantly, developing novel traits by genetic transformation. Using Agrobacterium-mediated transformation on somatic embryos, blight-resistant American chestnuts [Castanea dentata (Marsh.) Borkh.] have been developed as shoot cultures in plant tissue culture. Rooting tissue-cultured shoots and acclimatizing the rooted plantlets are key steps in tree production. In this study, in vitro and ex vitro rooting methods were compared. The ex vitro method resulted in a lower initial rooting percentage but an overall higher survival percentage, resulting in higher potted plant production. The higher survival was likely due to partial acclimatization taking place before the plantlets were transplanted into potting mix. After 8 weeks, plantlets rooted via the ex vitro method were taller, and had more, and larger, leaves than the in vitro-rooted plantlets. These trees are currently in high demand for inoculation studies for federal regulatory review and eventually for restoration of this keystone species to its native habitat.

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals Clonal bamboo production based on in vitro culture

2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Anatálya dos Santos Ribeiro ◽  
Alexssandra Jéssica Rondon de Figueiredo ◽  
Gabriela Cristina Rech Tormen ◽  
André Luís Lopes da Silva ◽  
Wellington Ferreira Campos ◽  
...  
Keyword(s):  
Activated Carbon ◽  
Large Scale ◽  
Culture Medium ◽  
Clonal Plant ◽  
Adventitious Rooting ◽  
Clonal Plants ◽  
Ex Vitro ◽  
Bambusa Vulgaris ◽  
In Vitro Establishment

Bamboo species are an alternative for the composition of forest plantations. However, their potential has not been explored due to the hard time in producing large-scale clonal plants. Thus, the aim this work was to evaluate the in vitro establishment, bud multiplication and ex vitro rooting of Bambusa vulgaris. The first experiment tested different systemic and contact fungicide solutions, based on exposure time, during the establishment phase. Established explants were subjected to evaluation of residual fungicide effect on subcultures during the multiplication and elongation phases. The second experiment evaluated the influence of activated carbon on ex vitro survival and on adventitious rooting. Explant immersion in liquid culture medium added with 1.0 mL of fungicide for 120 hours has favored the in vitro establishment and reduced fungal contamination. On the other hand, it favored the shoot emission of shoots per explant during the multiplication phase. Both rooting induction culture medium and mini-incubator system use were effective in enabling adventitious root formation. The presence of activated carbon in the rooting induction culture medium resulted in a higher clonal plant survival rate.  

scholarly journals Study of anatomical feature of in vitro and ex vitro regeneration plant of Sophora Alopecuroides l.

2019 ◽  
pp. 32-40
Author(s):  
Tsolmon M ◽  
Bayarmaa B ◽  
Oyunbileg Yu
Keyword(s):  
Success Rate ◽  
Ex Vitro Rooting ◽  
Vascular Cylinder ◽  
Ex Vitro ◽  
Anatomical Feature ◽  
Plant Survival ◽  
Anatomical Changes ◽  
Sophora Alopecuroides

The success of shoot and rooting from the nodal shoot segments of Sophora alopecuroides L. were induced in vitro condition. However, transferring and acclimatizing the plantlets to under soil or ex vitro condition were difficult. This study investigated plant survival with anatomical changes in plantlets while transferring from in vitro to ex vitro conditions to investigate vascular cylinder variations. The ex vitro rooting of the in vitro regenerated shoots, after having been treated with 500 mg/L IBA, showed a success rate of 80 per cent. These plantlets were rooted and acclimatized simultaneously in ex vitro condition.

In vitro and ex vitro rooting of Siratia grosvenorii, a traditional medicinal plant

2009 ◽  
Vol 32 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Huabing Yan ◽  
Chunxiu Liang ◽  
Litao Yang ◽  
Yangrui Li
Keyword(s):  
Medicinal Plant ◽  
Ex Vitro Rooting ◽  
Ex Vitro
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