Phytotoxic Activity of Alkaloids in the Desert Plant Sophora alopecuroides

Sophora alopecuroides is known to produce relatively large amounts of alkaloids; however, their ecological consequences remain unclear. In this study, we evaluated the allelopathic potential of the main alkaloids, including aloperine, matrine, oxymatrine, oxysophocarpine, sophocarpine, sophoridine, as well as their mixture both in distilled H2O and in the soil matrix. Our results revealed that all the alkaloids possessed inhibitory activity on four receiver species, i.e., Amaranthus retroflexus, Medicago sativa, Lolium perenne and Setaria viridis. The strength of the phytotoxicity of the alkaloids was in the following order: sophocarpine > aloperine > mixture > sophoridine > matrine > oxysophocarpine > oxymatrine (in Petri dish assays), and matrine > mixture > sophocarpine > oxymatrine > oxysophocarpine > sophoridine > aloperine (in pot experiments). In addition, the mixture of the alkaloids was found to significantly increase the IAA content, MDA content and POD activity of M. sativa seedlings, whereas CTK content, ABA content, SOD activity and CAT activity of M. sativa seedlings decreased markedly. Our results suggest S. alopecuroides might produce allelopathic alkaloids to improve its competitiveness and thus facilitate the establishment of its dominance; the potential value of these alkaloids as environmentally friendly herbicides is also discussed.

Uncommon Bis-Amide Matrine-type Alkaloids From Sophora alopecuroides With Anti-inflammatory Effects

Four new alkaloids (1–4) belonging to rare examples of bis-amide matrine-type were isolated from the seeds of sophora alopecuroides. Their structures including absolute configuration were determined by extensive spectroscopic analysis, electronic circular dichroism (ECD) interpretation, and X-ray diffraction crystallography. Chemically, bis-amide matrine-type alkaloids can provide new molecular template for structural modification. Compounds 3–4 displayed obvious anti-inflammatory effects based on the inhibition of two key pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] in a dose-dependent manner, with IC50 values from 35.6 to 45.8 μm.

Ecological stoichiometric characteristics and influencing factors of carbon, nitrogen, and phosphorus in the leaves of Sophora alopecuroides L. in the Yili River Valley, Xinjiang

Background Sophora alopecuroides L. (S. alopecuroides L.) is a perennial herb widely distributed throughout Xinjiang, China. It is characterized by its rapid diffusion ability. Methods To reveal the ecological mechanism of the rapid spread of S. alopecuroides, and to elucidate the ecological stoichiometric characteristics of C, N, and P (and the influencing factors) in the leaves of S. alopecuroides, leaves were sampled from four habitats—forest, roadside, farmland, and desert—across the Yili River Valley. The variation rules of the ecological stoichiometric characteristics of C, N, and P in the leaves of S. alopecuroides were analyzed. Correlations between the ecological stoichiometric characteristics of leaves and environmental factors were examined using redundancy analysis (RDA). Results (1) The C, N, and P contents of S. alopecuroides leaves were 391.30–533.10 g/kg, 8.90–43.14 g/kg, and 0.71–2.04 g/kg, respectively, and the C/N, C/P, and N/P ratios were 10.34–4.94, 209.05–698.73, and 10.78–31.43 respectively. (2) The C content and C/P ratio of S. alopecuroides leaves were the highest in the desert habitat, leaf N content and N/P ratio were the highest in the forest habitat, leaf P content was the highest in the farmland habitat, and the leaf C/N ratio was the highest in the roadside habitat. (3) RDA showed that available potassium (AK) and pH were the main factors affecting the ecological stoichiometric characteristics of S. alopecuroides leaves in Yili Valley (p ≤ 0.05), and these factors were positively correlated with C, N, P, and N/P, and negatively correlated with C/P and C/N. AK was the dominant factor that affected the P content of S. alopecuroides leaves, and appropriate reduction of K fertilizer would be conducive to restraining the spread of S. alopecuroides. Soil C, N, P, and K content, soil organic matter (OM), nitrate nitrogen (NO3−-N), ammonium nitrogen (NH4+-N), and AK had no significant effect on the ecological stoichiometric characteristics of leaves (p > 0.05).

Analysis of Phytohormone Signal Transduction in Sophora alopecuroides under Salt Stress

Salt stress seriously restricts crop yield and quality, leading to an urgent need to understand its effects on plants and the mechanism of plant responses. Although phytohormones are crucial for plant responses to salt stress, the role of phytohormone signal transduction in the salt stress responses of stress-resistant species such as Sophora alopecuroides has not been reported. Herein, we combined transcriptome and metabolome analyses to evaluate expression changes of key genes and metabolites associated with plant hormone signal transduction in S. alopecuroides roots under salt stress for 0 h to 72 h. Auxin, cytokinin, brassinosteroid, and gibberellin signals were predominantly involved in regulating S. alopecuroides growth and recovery under salt stress. Ethylene and jasmonic acid signals may negatively regulate the response of S. alopecuroides to salt stress. Abscisic acid and salicylic acid are significantly upregulated under salt stress, and their signals may positively regulate the plant response to salt stress. Additionally, salicylic acid (SA) might regulate the balance between plant growth and resistance by preventing reduction in growth-promoting hormones and maintaining high levels of abscisic acid (ABA). This study provides insight into the mechanism of salt stress response in S. alopecuroides and the corresponding role of plant hormones, which is beneficial for crop resistance breeding.

Rapid screening of active components group with Topoisomerase I inhibitory activity in Sophora alopecuroides L. based on ultrafiltration coupled with UPLC-QTOF-MS

Background: Topoisomerase I (Topo I) is a key target of many antitumor drugs in vivo. Alkaloids in Sophora alopecuroides L. can reportedly inhibit Topo I activity, but the pharmacodynamic material basis has not yet been determined. Objective: The objective of this study is to rapidly identify active components group which inhibit Topo I in S. alopecuroides L. Methods: Affinity ultrafiltration-ultra-performance liquid chromatography-quadrupole time of flight-mass spectrometry (UF-UPLC-QTOF-MS) screening system based on Topo I protein was established to screen and isolate a total alkaloid fraction in S. alopecuroides L. Topo I inhibitory activity and anti-tuomor proliferation activity of the screened components were evaluated, and their molecular mechanisms were studied. Results: Six compounds bound specifically to Topo I were obtained. Further screening showed that matrine, cytisine, and sophoridine presented higher inhibitory activity on Topo I and were able to inhibit the proliferation of breast cancer MDA-MB-468 cells with IC50 values of 9.40 ± 1.12 mM, 17.4 ± 2.20 mM and 10.4 ± 1.37 mM, respectively. To the best of our knowledge, their dual molecular mechanisms against Topo I have been discussed here for the first time: (1) stabilization of Topo I-DNA complex and (2) inhibition or blocking of Topo I binding to DNA. Conclusion: Matrine, cytisine, and sophoridine from S. alopecuroides L. were defined as the active components group with Topo I inhibitory activity and their pharmacological mechanism was confirmed, which provided an important base for further research and development of antitumor components fromS. alopecuroides L.