Low-intensity pulsed ultrasound promotes the expression of immediate-early genes in mouse ST2 bone marrow stromal cells

AbstractLow-intensity pulsed ultrasound (LIPUS) is an established therapy for fracture healing where bone marrow stromal cells (BMSCs) migration is crucial to bone regeneration. This work focused on different performances of C-X-C-receptor 4 (CXCR4), integrin-1β and chemokine-chemokine receptor2 (CCR-2) in BMSCs migration by LIPUS stimulation. Single 20-min LIPUS treatment was applied to BMSCs during wound healing assay with or without the inhibitor AMD3100. The migration rate of BMSCs with LIPUS stimulation exhibited a higher closure rate than that of BMSCs without LIPUS stimulation, which was 1.89 μm/h and 1.38 μm/h, respectively. After LIPUS stimulation, significant elevation of the expression of CXCR4, integrin-1β and CCR-2 was observed. When AMD3100 was added, the migration rate of the BMSCs was obviously declined with or without LIPUS treatment. Furthermore, the expression of CXCR4 was significantly down-regulated by AMD3100, while integrin-1β and CCR-2 were less affected. It suggested that the enhancement of the migration of the BMSCs by LIPUS was inhibited by AMD3100. The results confirmed that LIPUS stimulation was able to activate and improve migration of BMSCs. Nevertheless, CXCR4 and both integrin-1β and CCR-2 had different roles in BMSCs migration after LIPUS treatment.

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Osteogenic differentiation of rat bone marrow stromal cells by various intensities of low-intensity pulsed ultrasound

Ultrasonics ◽

10.1016/j.ultras.2010.09.004 ◽

2011 ◽

Vol 51 (3) ◽

pp. 281-288 ◽

Cited By ~ 89

Author(s):

S.R. Angle ◽

K. Sena ◽

D.R. Sumner ◽

A.S. Virdi

Keyword(s):

Bone Marrow ◽

Osteogenic Differentiation ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Pulsed Ultrasound ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity ◽

Rat Bone

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Low-intensity pulsed ultrasound (LIPUS) and cell-to-cell communication in bone marrow stromal cells

Ultrasonics ◽

10.1016/j.ultras.2011.01.007 ◽

2011 ◽

Vol 51 (5) ◽

pp. 639-644 ◽

Cited By ~ 29

Author(s):

Kotaro Sena ◽

Siddhesh R. Angle ◽

Arihiko Kanaji ◽

Chetan Aher ◽

David G. Karwo ◽

...

Keyword(s):

Bone Marrow ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Cell Communication ◽

Marrow Stromal Cells ◽

Pulsed Ultrasound ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity

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Effects of Low-Intensity Electromagnetic Fields on the Proliferation and Differentiation of Cultured Mouse Bone Marrow Stromal Cells

Physical Therapy ◽

10.2522/ptj.20110224 ◽

2012 ◽

Vol 92 (9) ◽

pp. 1208-1219 ◽

Cited By ~ 13

Author(s):

Cheng Zhong ◽

Xin Zhang ◽

Zhengjian Xu ◽

Rongxin He

Keyword(s):

Bone Marrow ◽

Cell Proliferation ◽

Electromagnetic Fields ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Control Group ◽

Mouse Bone Marrow ◽

Low Intensity ◽

Hematoxylin And Eosin

Background Electromagnetic fields (EMFs) used in stem-cell tissue engineering can help elucidate their biological principles. Objective The aim of this study was to investigate the effects of low-intensity EMFs on cell proliferation, differentiation, and cycle in mouse bone marrow stromal cells (BMSCs) and the in vivo effects of EMFs on BMSC. Methods Harvested BMSCs were cultured for 3 generations and divided into 4 groups. The methylthiotetrazole (MTT) assay was used to evaluate cell proliferation, and alkaline phosphatase activity was measured via a colorimetric assay on the 3rd, 7th, and 10th days. Changes in cell cycle also were analyzed on the 7th day, and bone nodule formation was analyzed on the 12th day. Additionally, the expression of the collagen I gene was examined by reverse transcription-polymerase chain reaction (RT-PCR) on the 10th day. The BMSCs of the irradiated group and the control group were transplanted into cortical bone of different mice femurs separately, with poly(lactic-co-glycolic acid) (PLGA) serving as a scaffold. After 4 and 8 weeks, bone the bone specimens of mice were sliced and stained by hematoxylin and eosin separately. Results The results showed that EMFs (0.5 mT, 50 Hz) accelerated cellular proliferation, enhanced cellular differentiation, and increased the percentage of cells in the G2/M+S (postsynthetic gap 2 period/mitotic phase + S phase) of the stimulation. The EMF-exposed groups had significantly higher collagen I messenger RNA levels than the control group. The EMF + osteogenic medium–treated group readily formed bone nodules. Hematoxylin and eosin staining showed a clear flaking of bone tissue in the irradiated group. Conclusion Irradiation of BMSCs with low-intensity EMFs (0.5 mT, 50 Hz) increased cell proliferation and induced cell differentiation. The results of this study did not establish a stricter animal model for studying osteogenesis, and only short-term results were investigated. Further study of the mechanism of EMF is needed.

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