Hsp60/10 and sHsp families of heat shock protein genes in rainbow trout (Oncorhynchus mykiss) and their expression under heat stress

To study the response to heat stress in rainbow trout Oncorhynchus mykiss (Walbaum, 1972), the expression of heat shock protein genes Hsp90a2b and Hsp10 in selected tissues and apoptosis of blood cells were investigated at water temperatures of 18, 21, 23, 24, 25 and 26°C. Expressions of Hsp90a2b in the gill, mid-kidney, brain, heart and muscle were generally upregulated with increasing temperature. Hsp90a2b expression in the liver increased rapidly at 21°C and then showed a significant decrease and finally a significant increase at 25°C and 26°C (p<0.05). Hsp10 expression showed an irregular but overall increasing tendency in gill, spleen and mid-kidney and a wave-like increasing pattern in liver, heart and muscle. Levels of Hsp90a2b at 25°C and Hsp10 at 26°C in all tissues were always significantly higher compared to 18°C (p<0.05). The apoptosis rate at 21°C was significantly higher than that at 18°C, followed by a fall and was the highest at 25°C. All these data indicated that the heat shock response already occurred at 21°C which induced expressions of Hsp90a2b and Hsp10 as well as blood cells apoptosis and 25°C may be a key temperature for stress response in rainbow trout.

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Effects of heat stress on biochemical parameters and heat shock protein family A (Hsp70) member 5 (HSPA5) mRNA expression in rainbow trout (Oncorhynchus mykiss)

Marine and Freshwater Research ◽

10.1071/mf18029 ◽

2018 ◽

Vol 69 (11) ◽

pp. 1674 ◽

Cited By ~ 5

Author(s):

Binpeng Xia ◽

Zhe Liu ◽

Yanjing Zhou ◽

Yongjie Wang ◽

Jinqiang Huang ◽

...

Keyword(s):

Heat Stress ◽

Rainbow Trout ◽

Heat Shock ◽

Heat Shock Protein ◽

Oncorhynchus Mykiss ◽

Mrna Expression ◽

Biochemical Parameters ◽

Cold Water ◽

Protein Family ◽

Rainbow Trout Oncorhynchus Mykiss

Rainbow trout (Oncorhynchus mykiss) is a cold-water species of salmonid, and high temperatures are a significant threat to its aquaculture. In order to understand the degree of the heat stress response and the mechanisms involved, full-sibling inbred O. mykiss individuals were sampled at 18, 21, 23, 24, 25 and 26°C to investigate changes in some serum biochemical parameters, as well as in the mRNA expression of heat shock protein family A (Hsp70) member 5 (HSPA5; also known as glucose regulated protein 78 (GRP78)) in different tissues (liver, mid-kidney, heart, spleen and brain). At 21°C, there was a significant increase in the spleen macrophage respiratory burst and a significant decrease in superoxide dismutase activity compared with 18°C (P<0.05). Malondialdehyde peaked at 23°C, whereas alanine transaminase and aspartate aminotransferase activity were both twofold higher at 25 and 26°C compared with that at 18°C. The Ca2+, Mg2+, PO43– and glucose (Glu) content of serum declined significantly at 21°C relative to 18°C (P<0.05). The expression of HSPA5 mRNA responded in a temperature- and tissue-specific manner to heat stress. Except for in the spleen, HSPA5 mRNA expression was significantly higher in all tissues at 25 and 26°C than that at 18°C (P<0.05). These results indicate that heat stress causes oxidative damage, decreases the Ca2+, Mg2+, PO43– and Glu content of serum and induces HSPA5 mRNA expression.

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