On-Bone Fixation of Free Gingival Graft Induces an Osteoinductive Effect in Human Alveolar Bone

Author(s):  
S. S. Edranov ◽  
N. Yu. Matveeva ◽  
S. G. Kalinichenko
Author(s):  
S. S. Edranov ◽  
◽  
N. Yu. Matveeva ◽  
S. G. Kalinichenko ◽  
◽  
...  

Author(s):  
S. S. Edranov ◽  
Natalya Yu. Matveeva ◽  
S. G. Kalinichenko

Aim: To study the localization of stem stromal cells on the surface of a free gingival graft. Material and methods: We studied the immunolocalization of stem cell markers (CD29, CD44 and osteocalcin) the mucosa plate and bone tissue of the lower jaw of outbred miniature pigs with bone fixation of a free gingival graft. Results: In the third month after the operation, a new bone tissue is formed in the contact area, characterized by formed bone plates, osteons, and a system of Havers and Volkman channels. When studying the immunolocalization of CD29, CD44, and osteocalcin, we detected a heterogeneous distribution. CD29and CD44-immunopositive cells prevail at the border of the newly formed bone and its own gingival plate, as well as in the cavity of the Havers canals. Osteocalcin-positive cells are localized mainly in the layer of common plates, on the surface of the bone plates of osteons surrounding the Havers channel and in its cavity, which are in close proximity to the graft. Conclusion: Bone fixation of SDT remodels the alveolar bone, showing signs of a reparative process. The presence of expression of CD29 and CD44 in bone tissue indicates the activation of mesenchymal cambial cells, which differentiate into osteocalcin-containing osteoblasts. The inducing role of a free gingival graft in the formation of a new bone is determined by the ability of its cells to synthesize trophic factors stimulating the proliferation and differentiation of MSC and vascular growth. Thus, the model on miniature pigs is a useful tool for studying this process.


2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Nurcan Tezci ◽  
Suleyman Emre Meseli ◽  
Burcu Karaduman ◽  
Serap Dogan ◽  
Sabri Hasan Meric

Background. Oral fibromas are benign, asymptomatic, smooth surfaced, firm structured tumoral lesions that originate from gingival connective tissue or periodontal ligament. Histologically, they are nodular masses characterized by a dense connective tissue, surrounded by stratified squamous epithelium.Case Report. This case report includes the clinical, radiographical, and histological findings and periodontal treatment of a 38-year-old female patient having painless swelling on the gingiva. Intraoral examination revealed a fibrotic, sessile, smooth surfaced gingival overgrowth interdentally between the teeth #13 and #14. Radiographical findings were normal. Initial periodontal treatment (IPT) was applied including oral hygiene instructions, scaling, and root planing. Following IPT, the lesion (0.7 × 0.6 × 0.4 cm) was excised and examined histopathologically. Subsequently, flap operation was performed to have an access to alveolar bone. Surgical site was reconstructed with free gingival graft obtained from hard palate. Hematoxylin-eosin stained sections revealed a nodular mass composed by dense collagen fibers in lamina propria covered by a stratified squamous epithelium, which were consistent with fibroma. Gingival healing was uneventful and without any recurrence during the 12-month follow-up.Conclusions. In order to achieve optimal functional and aesthetical outcomes, free gingival graft can be used for the reconstruction of the wound site after the excision of the fibroma.


Author(s):  
S. S Edranov ◽  
R. A Kerzikov

This review analyzes the current state of the problem of creating the area of keratinized and attached gingiva in dental implantology. Apically positioned flap with a free gingival graft (FGG) placement is considered the gold standard in the oral soft tissue plastic surgery. It is noted that the periosteal or connective tissue recipient bed is an important prerequisite to graft revascularization and survival. FGGs placed on the periosteum often display clinical mobility while grafts fixed on denuded alveolar bone predictably create a firmly attached band of keratinized gingiva. The development of specific techniques and methods for fixing the FGG on bone bed have special priority. Exploring rigid fixation of the graft, as well as cellular and molecular mechanisms that determine the dynamics of the regenerative processes at the graft-bed interface has great clinical potential.


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