Effective strategy for pyramiding three bacterial blight resistance genes into fine grain rice cultivar, Samba Mahsuri, using sequence tagged site markers

2010 ◽  
Vol 32 (7) ◽  
pp. 989-996 ◽  
Author(s):  
Kameswara Rao Kottapalli ◽  
M. Lakshmi Narasu ◽  
Kshirod K. Jena
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Rice Cultivar ◽  
Blight Resistance ◽  
Effective Strategy ◽  
Bacterial Blight Resistance ◽  
Sequence Tagged Site ◽  
Fine Grain ◽  
Site Markers

Pyramiding of Three Bacterial Blight Resistance Genes in Rice Cultivar Using Marker Assisted Selection

2018 ◽  
Vol 9 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Kadu Tanvi P. ◽  
◽  
Kale Sonam S. ◽  
Chavan Narendra R. ◽  
Toshy Agrawal ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Rice Cultivar ◽  
Blight Resistance ◽  
Bacterial Blight Resistance

scholarly journals Bacterial Blight Resistance Genes Pyramided in Mid-Late Maturing Rice Cultivar ‘Sinjinbaek’ with High Grain Quality

2019 ◽  
Vol 51 (3) ◽  
pp. 263-276 ◽  
Author(s):  
Hyun-Su Park ◽  
Ki-Young Kim ◽  
Man-Kee Baek ◽  
Young-Chan Cho ◽  
Bo-Kyeong Kim ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Grain Quality ◽  
Rice Cultivar ◽  
Blight Resistance ◽  
Bacterial Blight Resistance

scholarly journals Improvement of bacterial blight resistance of the popular variety, Nellore Mahsuri, NLR34449 through marker-assisted breeding

2020 ◽  
Author(s):  
Aleena D ◽  
Padma V ◽  
Rekha G ◽  
Dilip T ◽  
Punniakotti E ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Grain Quality ◽  
Rice Variety ◽  
Blight Resistance ◽  
Bacterial Blight Resistance ◽  
Marker Assisted Breeding ◽  
Fine Grain ◽  
High Level ◽  
Marker Assisted Backcross Breeding

Abstract To combat the dreaded diseases in rice like bacterial blight and blast, host plant resistance has been advocated as a sustainable method. Through the present study, we have successfully incorporated three major bacterial blight (BB) resistance genes viz., Xa21, xa13 and xa5 into NLR3449, a high yielding, blast resistant, fine-grain type popular rice variety through marker-assisted backcross breeding. Foreground selection was carried out using PCR based, gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13) and xa5FM (xa5) at each generation of backcrossing, while 127 polymorphic SSR markers spanning on 12 chromosomes were used for background selection and backcrossing was limited to two rounds. At BC2F1 generation, a single plant (NLR-87-10) with 89.9% recovery and possessing all the three bacterial blight resistance genes was forwarded to BC2F2 generation. A solitary BC2F2 plant viz., NLR-87-10-106 possessing all the three resistance genes and > 90% genome recovery was identified and advanced through selfing till BC2F4 generation by adopting pedigree method. Three best lines at BC2F4 lines, possessing high level of resistance against bacterial blight and blast and equivalent or superior to NLR 34449 in terms of yield, grain quality and agro-morphological traits have been identified and advanced for multi-location trials.

scholarly journals Marker assisted improvement of low soil phosphorus tolerance in the bacterial blight resistant, fine-grain type rice variety, Improved Samba Mahsuri

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
H. K. Mahadeva Swamy ◽  
M. Anila ◽  
R. R. Kale ◽  
G. Rekha ◽  
V. P. Bhadana ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Soil Phosphorus ◽  
Rice Variety ◽  
Blight Resistance ◽  
Functional Markers ◽  
Bacterial Blight Resistance ◽  
Soil P ◽  
Fine Grain ◽  
Normal Soil

AbstractImproved-Samba-Mahsuri (ISM), a high-yielding, popular bacterial blight resistant (possessing Xa21, xa13, and xa5), fine-grain type, low glycemic index rice variety is highly sensitive to low soil phosphorus (P). We have deployed marker-assisted backcross breeding (MABB) approach for targeted transfer of Pup1, a major QTL associated with low soil P tolerance, using Swarna as a donor. A new co-dominant marker, K20-1-1, which is specific for Pup1 was designed and used for foreground selection along with functional markers specific for the bacterial blight resistance genes, Xa21, xa13, and xa5. A set of 66 polymorphic SSR marker were used for the background selection along with a pair of flanking markers for the recombination selection in backcross derived progenies and in BC2F2 generation, 12 plants, which are homozygous for Pup1, all the three bacterial blight resistance genes and possessing agro-morphological traits equivalent to or better than ISM were selected and selfed to produce BC2F3s. They were evaluated in plots with low soil P and normal soil P at ICAR-IIRR, Hyderabad for their low soil P tolerance, and bacterial blight resistance and superior lines were advanced to BC2F6. One of the lines, when tested at multiple locations in India was found promising under both normal as well as low soil P conditions.

Multiplex PCR genotyping for five bacterial blight resistance genes applied to marker-assisted selection in rice (Oryza sativa)

2016 ◽  
Vol 135 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Ruishen Yap ◽  
Yu-Chia Hsu ◽  
Yong-Pei Wu ◽  
Yann-Rong Lin ◽  
Chei-Wei Kuo
Keyword(s):  
Oryza Sativa ◽  
Multiplex Pcr ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Bacterial Blight Resistance ◽  
Pcr Genotyping

scholarly journals Development of Breeding Lines for Early Maturing Glutinous japonica Rice carrying Bacterial Blight Resistance Genes, Xa2, Xa3, xa5, and Xa21

2014 ◽  
Vol 46 (3) ◽  
pp. 195-202
Author(s):  
Hyun-Su Park ◽  
Ki-Young Kim ◽  
Woo-Jae Kim ◽  
Jong-Cheol Ko ◽  
Jeong-Kwon Nam ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Japonica Rice ◽  
Bacterial Blight Resistance ◽  
Breeding Lines ◽  
Early Maturing

scholarly journals Epidemiological Effect of Gene Deployment Strategies on Bacterial Blight of Rice

1997 ◽  
Vol 87 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Hafiz U. Ahmed ◽  
Maria R. Finckh ◽  
Rizal F. Alfonso ◽  
Christopher C. Mundt
Keyword(s):  
Resistance Genes ◽  
Partial Resistance ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Resistant Line ◽  
Bacterial Blight Resistance ◽  
Progress Curve ◽  
Two Component ◽  
The Mean ◽  
Bacterial Blight Of Rice

Experiments were conducted in farmers' fields at two locations of the irrigated lowlands of Laguna province in southern Luzon island, Philippines, during the wet seasons of 1993 and 1994. Nine rice populations were studied including pure stands, two-component mixtures, two-gene combinations of backcrossed lines containing varying combinations of the bacterial blight resistance genes Xa-4, xa-5, and Xa-10, and a non-isogenic cultivar containing Xa-4 and partial resistance to bacterial blight. The area under the disease progress curve (AUDPC) of both gene combinations studied was significantly less than the single most effective gene of each combination deployed singly. A mixture of a susceptible and a resistant line expressed an AUDPC significantly less than the mean of its component pure stands, but two other mixtures did not. The cultivar IR20, which contains both Xa-4 and partial resistance, reduced the AUDPC by about two-thirds as compared with IR-BB4, which contains Xa-4 and little or no partial resistance.

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