Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett–Burman design combined with response surface methodology

2021 ◽  
Author(s):  
Lingbin Yan ◽  
Zhulan Zhang ◽  
Yin Zhang ◽  
Huangjian Yang ◽  
Guanrong Qiu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Fermentation Medium ◽  
Streptomyces Tsukubaensis ◽  
Burman Design ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

Enhanced Production of Mycelia by the Medicinal Mushroom Cordyceps militaris Using Plackett-Burman Design and Response Surface Methodology

2011 ◽  
Vol 138-139 ◽  
pp. 1209-1214
Author(s):  
Xiao Yu Liu ◽  
Fan Xing Meng ◽  
Yi Bo Zhang ◽  
Huan He ◽  
Wei Han ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Cordyceps Militaris ◽  
Enhanced Production ◽  
Burman Design ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

Response surface methodology (RSM) was used for statistical optimization of fermentation medium that influenced the yield of endo-polysaccharide from cultivated mycelia of Cordyceps militaris. First, the Plackett-Burman design was used to evaluate the effects of ten variables including glucose, maltose, peptone, yeast extract, KH2PO4, MgSO4, CaCl2, VB1, inoculum density and medium capacity. Among these variables, glucose, peptone and yeast extract were identified to have the significant effects. Subsequently, response surface methodology based on a five-level three-factor central composite design was employed to determine the maximum dry weight (DW) of mycelial biomass at optimum concentration of glucose, peptone and yeast extract. The mycelia growth was found to correlate to the three parameters that could be represented by second-order polynomial models. The optimal values of the three parameters were determined as 4.62% glucose, 3.36% peptone and 0.43% yeast extract. The prediction DW was 23.727g/L. The actual experimental results were in agreement with the prediction.

Medium Optimization for the Antibacterial Substances Production from Brevibacillus brevis XDH Using Response Surface Methodology

2011 ◽  
Vol 345 ◽  
pp. 355-360 ◽  
Author(s):  
Zhen Song ◽  
Xun Li Liu ◽  
Chang Xu Lu ◽  
Jian Yu ◽  
Rui Cheng Ju ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Antibacterial Activity ◽  
Response Surface ◽  
Medium Optimization ◽  
Fermentation Medium ◽  
Steepest Ascent ◽  
High Antibacterial Activity ◽  
Burman Design ◽  
Brevibacillus Brevis ◽  
Plackett Burman Design

Brevibacillus Brevis XDH is an antagonistic bacterium with high antibacterial activity against many pathogens. Fermentation medium for the production of the antibacterial substances was statistically optimized by Plackett-Burman design, steepest ascent experiments and response surface methodology. The optimized medium was composed of (g/L): glucose 15.6, soybean meal 28.4, starch 6, CaCO3 2 and MgSO4 4. After optimization, the production of antibacterial metabolites increased more than 70% than that of control.

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