Melatonin enhances the inhibitory effect of aminoglutethimide on aromatase activity in MCF-7 human breast cancer cells

2005 ◽  
Vol 94 (3) ◽  
pp. 249-254 ◽  
Author(s):  
Carlos Martínez-Campa ◽  
Alicia González ◽  
Maria Dolores Mediavilla ◽  
Carolina Alonso-González ◽  
Emilio J. Sánchez-Barceló ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Inhibitory Effect ◽  
Aromatase Activity ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

scholarly journals Studies on Multifunctional Effect of All-Trans Retinoic Acid (ATRA) on Matrix Metalloproteinase-2 (MMP-2) and Its Regulatory Molecules in Human Breast Cancer Cells (MCF-7)

2009 ◽  
Vol 2009 ◽  
pp. 1-13 ◽  
Author(s):  
Anindita Dutta ◽  
Triparna Sen ◽  
Aniruddha Banerji ◽  
Shamik Das ◽  
Amitava Chatterjee
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Gelatin Zymography ◽  
Inhibitory Effect ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Atra Treatment ◽  
Mcf 7

Background. Vitamin A derivative all-trans retinoic acid (ATRA) is considered as a potent chemotherapeutic drug for its capability of regulating cell growth and differentiation. We studied the effect of ATRA on MMP-2 in MCF-7, human breast cancer cells, and the probable signaling pathways which are affected by ATRA on regulating pro-MMP-2 activity and expression.Methods. Gelatin zymography, RT-PCR, ELISA, Western blot, Immunoprecipitation, and Cell adhesion assay are used.Results. Gelatin zymography showed that ATRA caused a dose-dependent inhibition of pro-MMP-2 activity. ATRA treatment downregulates the expression of MT1-MMP, EMMPRIN, FAK, NF-kB, and p-ERK. However, expression of E-cadherin, RAR, and CRABP increased upon ATRA treatment. Binding of cells to extra cellular matrix (ECM) protein fibronectin reduced significantly after ATRA treatment.Conclusions. The experimental findings clearly showed the inhibition of MMP-2 activity upon ATRA treatment. This inhibitory effect of ATRA on MMP-2 activity in human breast cancer cells (MCF-7) may result due to its inhibitory effect on MT1-MMP, EMMPRIN, and upregulation of TIMP-2. This study is focused on the effect of ATRA on MMP, MMP-integrin-E-cadherin interrelationship, and also the effect of the drug on different signaling molecules which may involve in the progression of malignant tumor development.

A nuclear receptor system constituted by RAR and RXR induces aromatase activity in MCF-7 human breast cancer cells

2000 ◽  
Vol 166 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Yi-Ming Mu ◽  
Toshihiko Yanase ◽  
Yoshihiro Nishi ◽  
Nobutaka Hirase ◽  
Kiminobu Goto ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Nuclear Receptor ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Aromatase Activity ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Receptor System ◽  
Mcf 7

Melatonin modulates aromatase activity in MCF-7 human breast cancer cells

2005 ◽  
Vol 38 (2) ◽  
pp. 136-142 ◽  
Author(s):  
Samuel Cos ◽  
Carlos Martinez-Campa ◽  
Maria D. Mediavilla ◽  
Emilio J. Sanchez-Barcelo
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Aromatase Activity ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7
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