Human dental pulp stem cells cultured onto dentin derived scaffold can regenerate dentin-like tissue in vivo

Tracking transplanted stem cells is necessary to clarify cellular properties and improve transplantation success. In this study, we investigate the effects of fluorescent superparamagnetic iron oxide particles (SPIO) (Molday ION Rhodamine-B™, MIRB) on biological properties of human dental pulp stem cells (hDPSCs) and monitor hDPSCs in vitro and in vivo using magnetic resonance imaging (MRI). Morphological analysis showed that intracellular MIRB particles were distributed in the cytoplasm surrounding the nuclei of hDPSCs. 12.5–100 μg/mL MIRB all resulted in 100% labeling efficiency. MTT showed that 12.5–50 μg/mL MIRB could promote cell proliferation and MIRB over 100 μg/mL exhibited toxic effect on hDPSCs. In vitro MRI showed that 1 × 106cells labeled with various concentrations of MIRB (12.5–100 μg/mL) could be visualized. In vivo MRI showed that transplanted cells could be clearly visualized up to 60 days after transplantation. These results suggest that 12.5–50 μg/mL MIRB is a safe range for labeling hDPSCs. MIRB labeled hDPSCs cell can be visualized by MRI in vitro and in vivo. These data demonstrate that MIRB is a promising candidate for hDPSCs tracking in hDPSCs based dental pulp regeneration therapy.

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Purmorphamine increased adhesion, proliferation and expression of osteoblast phenotype markers of human dental pulp stem cells cultured on beta-tricalcium phosphate

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2016.05.016 ◽

2016 ◽

Vol 82 ◽

pp. 432-438 ◽

Cited By ~ 8

Author(s):

Maryam Rezia Rad ◽

Moein Khojaste ◽

Mehrnoosh Hasan Shahriari ◽

Saeed Asgary ◽

Arash Khojasteh

Keyword(s):

Stem Cells ◽

Tricalcium Phosphate ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Beta Tricalcium Phosphate ◽

Human Dental Pulp ◽

Phenotype Markers ◽

Cells Cultured

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Simvastatin Induces the Odontogenic Differentiation of Human Dental Pulp Stem Cells In Vitro and In Vivo

Journal of Endodontics ◽

10.1016/j.joen.2008.11.024 ◽

2009 ◽

Vol 35 (3) ◽

pp. 367-372 ◽

Cited By ~ 53

Author(s):

Yosuke Okamoto ◽

Wataru Sonoyama ◽

Mitsuaki Ono ◽

Kentaro Akiyama ◽

Takuo Fujisawa ◽

...

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Odontogenic Differentiation ◽

Human Dental Pulp

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Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions

Stem Cell Research & Therapy ◽

10.1186/s13287-017-0761-5 ◽

2018 ◽

Vol 9 (1) ◽

Cited By ~ 12

Author(s):

Mai Mochizuki ◽

Taka Nakahara

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Culture Methods ◽

Serum Free ◽

Human Dental Pulp

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Magnetic Resonance Imaging of Human Dental Pulp Stem Cells in Vitro and in Vivo

Cell Transplantation ◽

10.3727/096368912x657774 ◽

2013 ◽

Vol 22 (10) ◽

pp. 1813-1829 ◽

Cited By ~ 24

Author(s):

T. Struys ◽

A. Ketkar-Atre ◽

P. Gervois ◽

C. Leten ◽

P. Hilkens ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Stem Cells ◽

Magnetic Resonance ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Resonance Imaging ◽

Human Dental Pulp

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LncRNA H19 Promotes Odontoblastic Differentiation of Human Dental Pulp Stem Cells by Regulating miR-140-5p and BMP-2/FGF9

10.21203/rs.3.rs-16133/v1 ◽

2020 ◽

Author(s):

Jialin Zhong ◽

Xinran Tu ◽

Yuanyuan Kong ◽

Liyang Guo ◽

Baishun Li ◽

...

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Biological Role ◽

Luciferase Reporter ◽

Qrt Pcr ◽

Odontoblastic Differentiation ◽

Human Dental Pulp

Abstract Background: Increasing evidence has revealed that long non-coding RNAs (lncRNAs) exert critical roles in biological mineralization. As a critical process for dentin formation, odontoblastic differentiation is regulated by complex signaling networks. The present study aimed to investigate the biological role and regulatory mechanisms of lncRNA-H19 (H19) in regulating the odontoblastic differentiation of human dental pulp stem cells (hDPSCs). Methods: We performed lncRNA microarray assay to reveal the expression patterns of lncRNAs involved in odontoblastic differentiation. H19 was identified and verified by qRT-PCR. The gain- and loss-of-function studies were performed to investigate the biological role of H19 in regulating odontoblastic differentiation of hDPSCs in vitro and in vivo. Odontoblastic differentiation was evaluated through qRT-PCR, Western blot and Alizarin Red S staining. Bioinformatics analysis identified that H19 could directly interact with miR-140-5p, which was further verified by luciferase reporter assay. After overexpression of miR-140-5p in hDPSCs, odontoblastic differentiation was determined. Moreover, the potential target genes of miR-140-5p were investigated and the biological functions of BMP-2 and FGF9 in hDPSCs were verified. Co-transfection experiments were conducted to validate miR-140-5p was involved in H19-mediated odontoblastic differentiation in hDPSCs.Results: The expression of H19 was significantly up-regulated in hDPSCs undergoing odontoblastic differentiation. Overexpression of H19 stimulated odontoblastic differentiation in vitro and in vivo, whereas down-regulation of H19 revealed the opposite effect. H19 binds directly to miR-140-5p and overexpression of miR-140-5p inhibited odontoblastic differentiation of hDPSCs. H19 acted as a miR-140-5p sponge, resulting in regulated the expression of BMP-2 and FGF9. Overexpression of H19 abrogated the inhibitory effect of miR-140-5p on odontoblastic differentiation.Conclusion: Our data revealed that H19 plays a positive regulatory role in odontoblastic differentiation of hDPSCs through miR-140-5p/BMP-2/FGF9 axis, suggesting that H19 may be a stimulatory regulator of odontogenesis.

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