Shikonin inhibits neuronal apoptosis via regulating endoplasmic reticulum stress in the rat model of double-level chronic cervical cord compression

2022 ◽  
Author(s):  
Min Yao ◽  
Gan Li ◽  
Long-yun Zhou ◽  
Zhong Zheng ◽  
Yue-li Sun ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Rat Model ◽  
Neuronal Apoptosis ◽  
Cord Compression ◽  
Cervical Cord ◽  
Cervical Cord Compression

scholarly journals Local Delivery of β-Elemene Improves Locomotor Functional Recovery by Alleviating Endoplasmic Reticulum Stress and Reducing Neuronal Apoptosis in Rats with Spinal Cord Injury

2018 ◽  
Vol 49 (2) ◽  
pp. 595-609 ◽  
Author(s):  
Jingyu Wang ◽  
Heyangzi Li ◽  
Yucheng Ren ◽  
Ying Yao ◽  
Jue Hu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Rat Model ◽  
Neuronal Apoptosis ◽  
Ventral Horn ◽  
Tunel Staining ◽  
Cord Injury

Background/Aims: Spinal cord injury (SCI) is a serious global problem that leads to permanent motor and sensory deficits. This study explores the anti-apoptotic and neuroprotective effects of the natural extract β-elemene in vitro and in a rat model of SCI. Methods: CCK-8 assay was used to evaluate cell viability and lactate dehydrogenase assay was used to evaluate cytotoxicity. A model of cell injury was established using cobalt chloride. Apoptosis was evaluated using a fluorescence-activated cell sorting assay of annexin V-FITC and propidium iodide staining. A rat SCI model was created via the modified Allen’s method and Basso, Beattie, and Bresnahan (BBB) scores were used to assess locomotor function. Inflammatory responses were assessed via enzyme-linked immunosorbent assay (ELISA). Apoptotic and surviving neurons in the ventral horn were respectively observed via terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and Nissl staining. Western blotting was used to measure protein expression. Results: β-elemene (20 μg/ml) promoted cell viability by activating phosphorylation of the PI3K-AKT-mTOR pathway. β-elemene reduced CoCl2-induced cellular death and apoptosis by suppressing the expression levels of CHOP, cleaved-caspase 12, 78-kilodalton glucose-regulated protein, cleaved-caspase 3, and the Bax/Bcl-2 ratio. In the rat model of SCI, Nissl and TUNEL staining showed that β-elemene promoted motor neuron survival and reduced neuronal apoptosis in the spinal cord ventral horn. BBB scores showed that β-elemene significantly promoted locomotor behavioral recovery after SCI. In addition, β-elemene reduced the ELISA-detected secretion of interleukin (IL)-6 and IL-1β. Conclusion: β-elemene reduces neuronal apoptosis by alleviating endoplasmic reticulum stress in vitro and in vivo. In addition, β-elemene promotes locomotor function recovery and tissue repair in SCI rats. Thus, our study provides a novel encouraging strategy for the potential treatment of β-elemene in SCI patients.

Poster 364 Cervical Cord Compression Presenting with Lower Extremity Numbness and Weakness: A Case Report

PM&R ◽  
2014 ◽  
Vol 6 (9) ◽  
pp. S312-S313
Author(s):  
Maria Margarita Lopez ◽  
Manish Mammen ◽  
Joseph David ◽  
Sanjeev Agarwal ◽  
Hana Ilan
Keyword(s):  
Case Report ◽  
Lower Extremity ◽  
Cord Compression ◽  
Cervical Cord ◽  
Cervical Cord Compression

scholarly journals Functional outcome of posterior laminectomy in case of cervical myelopathy with multilevel cervical cord compression

2018 ◽  
Vol 4 (4) ◽  
pp. 510-514
Author(s):  
Dr. Siddharth D Parekh ◽  
Dr. Arvind B Goregaonkar ◽  
Dr. Anoop Dhamangaokar ◽  
Dr. Apratim R Deekshit ◽  
Dr. Umesh P Kanade
Keyword(s):  
Functional Outcome ◽  
Cervical Myelopathy ◽  
Cord Compression ◽  
Cervical Cord ◽  
Cervical Cord Compression ◽  
Posterior Laminectomy

scholarly journals Clinical Reasoning: A 58-year-old woman with systemic scleroderma and progressive cervical cord compression

Neurology ◽  
2018 ◽  
Vol 91 (13) ◽  
pp. e1262-e1264
Author(s):  
Philipp Karschnia ◽  
Robert K. Fulbright ◽  
Maxwell S. Laurans ◽  
Anita J. Huttner ◽  
Joachim M. Baehring
Keyword(s):  
Clinical Reasoning ◽  
Cord Compression ◽  
Systemic Scleroderma ◽  
Cervical Cord ◽  
Cervical Cord Compression
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