Identification of Rat Respiratory Mucosa Stem Cells and Comparison of the Early Neural Differentiation Potential with the Bone Marrow Mesenchymal Stem Cells In Vitro

Human bone marrow mesenchymal stem cells (hBM-MSCs) are the best characterized multipotent adult stem cells. Their self-renewal capacity, multilineage differentiation potential, and immunomodulatory properties have indicated that they can be used in many clinical therapies. In a previous work we studied the DNA methylation levels of hBM-MSC genomic DNA in order to delineate a kind of methylation signature specific for early and late passages of culture. In the present work we focused on the modification of the methylation profiles of the X chromosome and imprinted loci, as sites expected to be more stable than whole genome. We propose a model where cultured hBM-MSCs undergo random modifications at the methylation level of most CGIs, nevertheless reflecting the original methylation status. We also pointed out global genome-wide demethylation connected to the long-term culture and senescence. Modification at CGIs promoters of specific genes could be related to the decrease in adipogenic differentiation potential. In conclusion, we showed important changes in CGIs methylation due to long-termin vitroculture that may affect the differentiation potential of hBM-MSCs. Therefore it is necessary to optimize the experimental conditions forin vitroexpansion in order to minimize these epigenetic changes and to standardize safer procedures.

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Neural differentiation of bone marrow mesenchymal stem cells with human brain‐derived neurotrophic factor gene‐modified in functionalized self‐assembling peptide hydrogel in vitro

Journal of Cellular Biochemistry ◽

10.1002/jcb.26408 ◽

2018 ◽

Vol 120 (3) ◽

pp. 2828-2835 ◽

Cited By ~ 14

Author(s):

Hongbin Luo ◽

Changsheng Xu ◽

Zhiwei Liu ◽

Lin Yang ◽

Yunda Hong ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Human Brain ◽

Neural Differentiation ◽

Factor Gene ◽

Self Assembling ◽

Marrow Mesenchymal Stem Cells ◽

Peptide Hydrogel

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Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential

Cell Proliferation ◽

10.1111/j.1365-2184.2010.00694.x ◽

2010 ◽

Vol 43 (5) ◽

pp. 445-456 ◽

Cited By ~ 53

Author(s):

L.-Y. Sun ◽

D.-K. Hsieh ◽

W.-S. Syu ◽

Y.-S. Li ◽

H.-T. Chiu ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Cell Proliferation ◽

Mesenchymal Stem Cells ◽

Human Bone ◽

Human Bone Marrow ◽

In Vitro Differentiation ◽

Differentiation Potential ◽

Marrow Mesenchymal Stem Cells

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Comparison of Proliferation and Osteogenic Differentiation Potential of Rat Mandibular and Femoral Bone Marrow Mesenchymal Stem Cells In Vitro

Stem Cells and Development ◽

10.1089/scd.2019.0256 ◽

2020 ◽

Vol 29 (11) ◽

pp. 728-736

Author(s):

Chuanjie Li ◽

Feifan Wang ◽

Rong Zhang ◽

Pengyan Qiao ◽

Hongchen Liu

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Femoral Bone ◽

Differentiation Potential ◽

Marrow Mesenchymal Stem Cells ◽

Femoral Bone Marrow

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Culture and neural differentiation of rat bone marrow mesenchymal stem cells in vitro

Cell Biology International ◽

10.1016/j.cellbi.2007.02.006 ◽

2007 ◽

Vol 31 (9) ◽

pp. 916-923 ◽

Cited By ~ 56

Author(s):

Zhao Lei ◽

Lin Yongda ◽

Ma Jun ◽

Sun Yingyu ◽

Zeng Shaoju ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Neural Differentiation ◽

Marrow Mesenchymal Stem Cells ◽

Rat Bone

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Comparative Effects of Bone Marrow Mesenchymal Stem Cells on Lipopolysaccharide-Induced Microglial Activation

Oxidative Medicine and Cellular Longevity ◽

10.1155/2013/234179 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 9

Author(s):

Fan-Wei Tseng ◽

May-Jywan Tsai ◽

Li-Yu Yu ◽

Yu-Show Fu ◽

Wen-Cheng Huang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Adherent Cell ◽

Cns Injury ◽

Differentiation Potential ◽

Paracrine Effects ◽

Marrow Mesenchymal Stem Cells ◽

Major Surface

After injury to the CNS, microglia are rapidly activated and concentrated and trigger inflammatory reaction at the sites of injury. Bone marrow mesenchymal stem cells (BMMSC) represent attractive cell sources for treating CNS injury. Although anti-inflammatory and paracrine effects of grafted BMMSC have been shown, direct modulation of BMMSC on microgliain situremains unclear. The present work employsin vitrotranswell assay to characterize the effects of BMMSC on LPS-stimulated microglia. BMMSC are cultivated in serum and serum-free (sf) conditions, namely, BMMSC and BMMSC-sf. Both cultures express major surface markers specific for mesenchymal stem cells. However, the BMMSC-sf exhibit sphere-like structure with reduced expression of two adherent cell markers, CD29 and CD90. Compared to BMMSC-sf, BMMSC are fibroblast like and have faster differentiation potential into neural-like cells. Furthermore, BMMSC release significant levels of TIMP-1 and VEGF, regardless of being alone or in coculture. The downregulated MMP-9 mRNA may be caused by TIMP-1 secretion from BMMSC. Our cell culture system provides a powerful tool for investigating the molecular and cellular changes in microglia-BMMSC cocultures.

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