Rapid identification of Salmo trutta lineages by multiplex PCR utilizing primers tailored to discriminate single nucleotide polymorphisms (SNPs) of the mitochondrial control region

ABSTRACT The genus Brucella includes a number of species that are major animal pathogens worldwide and significant causes of zoonotic infections of humans. Traditional methods of identifying Brucella to the species level can be time-consuming, can be subjective, and can pose a hazard to laboratory personnel in the absence of suitable biocontainment facilities. Using a robust phylogenetic framework, a number of single-nucleotide polymorphisms (SNPs) that define particular species within the genus were identified. These SNPs were used to develop a multiplex SNP detection assay, based on primer extension technology, that can rapidly and unambiguously identify an isolate as a member of one of the six classical Brucella species or as a member of the recently identified marine mammal group.

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Multiplex PCR and Oligonucleotide Microarray for Detection of Single-Nucleotide Polymorphisms Associated with Plasmodium falciparum Drug Resistance

Journal of Clinical Microbiology ◽

10.1128/jcm.00081-08 ◽

2008 ◽

Vol 46 (7) ◽

pp. 2167-2174 ◽

Cited By ~ 13

Author(s):

G. Q. Zhang ◽

Y. Y. Guan ◽

H. H. Sheng ◽

B. Zheng ◽

S. Wu ◽

...

Keyword(s):

Drug Resistance ◽

Plasmodium Falciparum ◽

Single Nucleotide Polymorphisms ◽

Multiplex Pcr ◽

Oligonucleotide Microarray ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Rapid identification of single nucleotide polymorphisms and estimation of allele frequencies using sequence traces from DNA pools

Poultry Science ◽

10.1093/ps/85.7.1165 ◽

2006 ◽

Vol 85 (7) ◽

pp. 1165-1168 ◽

Cited By ~ 11

Author(s):

X. Ye ◽

S. McLeod ◽

D. Elfick ◽

J.C. Dekkers ◽

S.J. Lamont

Keyword(s):

Single Nucleotide Polymorphisms ◽

Rapid Identification ◽

Allele Frequencies ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Dna Pools

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Association of genetic variations in genes involved in glutathione metabolism with risk of development of uterine fibroids

Nauchno-prakticheskii zhurnal «Medicinskaia genetika» ◽

10.25557/2073-7998.2020.06.52-54 ◽

2020 ◽

pp. 52-54

Author(s):

О.К. Кудрявцева ◽

Е.М. Барышева ◽

И.Н. Вдовина ◽

А.А. Клиновская ◽

Е.А. Новикова ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Multiplex Pcr ◽

Uterine Fibroids ◽

Genetic Variations ◽

Glutathione Metabolism ◽

Healthy Controls ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Increased Risk ◽

Risk Of Disease

В исследование были включены 552 пациентки с миомой матки (ММ) и 337 женщин контрольной группы соответствующего возраста. Генотипирование однонуклеотидных полиморфизмов (rs713041 GPX4, rs4902346 GPX2, rs41303970 GCLM, rs17883901 GCLC, rs1050450 GPX1, rs1799811 и rs1695 GSTP1, rs2551715 GSR, rs1801310 GSS, rs4820599 GGT1, rs7674870 SLC7A11) было проведено методом ПЦР в режиме реального времени; генотипирование делеционных полиморфизмов (+/0 GSTM1 и +/0 GSTT1) было проведено методом мультиплексной ПЦР. С повышенным риском развития ММ ассоциировался rs7674870 SLC7A11 (OR=1,25, 95%CI=1,03-1,50; p=0,02). Полиморфизм rs2551715 GSR обладал протективным эффектом относительно развития заболевания (OR=0,83, 95%CI=0,70-0,99; p=0,04). A total of 552 females with uterine fibroids and 337 age-matched healthy controls were recruited for the study. Genotyping of single nucleotide polymorphisms (SNPs) of rs713041 GPX4, rs4902346 GPX2, rs41303970 GCLM, rs17883901 GCLC, rs1050450 GPX1, rs1799811 and rs1695 GSTP1, rs2551715 GSR, rs1801310 GSS, rs4820599 GGT1, rs7674870 SLC7A11 were done using Taq-Man-based assays. Genotyping of +/0 GSTM1 and +/0 GSTT1 polymorphisms were done using multiplex PCR. Polymorphism rs7674870 SLC7A11 was associated with increased risk of uterine fibroids (OR=1.25, 95%CI=1.03-1.50; p=0.02); rs2551715 GSR was associated with decreased risk of disease (OR=0.83, 95% CI=0.70-0.99; p=0.04)

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