Methyl Jasmonate Induce Enhanced Production of Soluble Biophenols in PANAX GINSENG Adventitious Roots from Commercial Scale Bioreactors

2005 ◽  
Vol 41 (6) ◽  
pp. 669-673 ◽  
Author(s):  
G. Sivakumar ◽  
K. Y. Paek
Keyword(s):  
Methyl Jasmonate ◽  
Panax Ginseng ◽  
Adventitious Roots ◽  
Enhanced Production ◽  
Commercial Scale

scholarly journals Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate Elicitation in Codonopsis pilosula

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 533 ◽  
Author(s):  
Jiao-jiao Ji ◽  
Qi Feng ◽  
Hai-feng Sun ◽  
Xue-jun Zhang ◽  
Xiao-xiao Li ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Adventitious Roots ◽  
Multiple Shoots ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Codonopsis Pilosula ◽  
Meja Treatment ◽  
Metabolite Accumulation ◽  
Culture Growth ◽  
Sucrose Level

Bioactive metabolites in Codonopsis pilosula are of particular interest as an immunostimulant. Methyl jasmonate (MeJA) plays an important role in the elicitation of metabolite biosynthesis. Here, we explored the response of metabolites to MeJA elicitation in C. pilosula adventitious roots and multiple shoots. The results showed that the biomass, polysaccharide, and lobetyolin content of adventitious roots exhibited the highest increases with 100 µmol·L−1 MeJA at the 16th day of subculture, whereas the atractylenolide III (a terpenoid) content increased extremely with 50 µmol·L−1 MeJA treatment at the 7th day of subculture. In addition, the biomass and lobetyolin content significantly increased at the 4th day after treatment. Similarly, the polysaccharide and lobetyolin content increased in multiple shoots. Further identification of different metabolites responding to MeJA by 1H-NMR showed an extremely significant increase of the lobetyolinin level, which coincided with lobetyolin. Accordingly, the precursor, fatty acids, showed a highly significant decrease in their levels. Furthermore, a significant increase in β-d-fructose-butanol glycoside was detected, which was accompanied by a decrease in the sucrose level. Accordingly, the enzyme genes responsible for terpenoid and carbohydrate biosynthesis, CpUGPase, and CpPMK, were up regulated. In conclusion, MeJA promoted culture growth and accelerated bioactive metabolite accumulation by regulating the expression of the metabolite biosynthesis related genes, CpUGPase and CpPMK in C. pilosula.

Proteomics-based Identification of Components in the Adventitious Roots of Panax Ginseng C. A. Mayer related to Energy Metabolism and Antibiotic Effects

2014 ◽  
Vol 22 (1) ◽  
pp. 167-182 ◽  
Author(s):  
Jin-Hyoung Cho ◽  
Young-Joo Jeon ◽  
Ra-Ham Lee ◽  
Jung-Hyun Shim ◽  
Jung-Il Chae
Keyword(s):  
Energy Metabolism ◽  
Panax Ginseng ◽  
Adventitious Roots

Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation

2009 ◽  
Vol 53 (3) ◽  
pp. 408-414 ◽  
Author(s):  
D. S. Kim ◽  
S. Y. Kim ◽  
I. Y. Jeong ◽  
J. -B. Kim ◽  
G. -J. Lee ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Adventitious Roots ◽  
Γ Irradiation
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